Search results for the GEO ID: GSE33109 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM819945 | GPL570 |
|
hN2_control_0 h_rep1
|
hN2 human neural cells not exposed to VX at the beginning of experiment
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_0 h_1
gene expression data of hN2 human neural cells not exposed to VX at the beginning of experiment
|
Sample_geo_accession | GSM819945
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819945/suppl/GSM819945_hN2_0_uM_0_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819946 | GPL570 |
|
hN2_control_0 h_rep2
|
hN2 human neural cells not exposed to VX at the beginning of experiment
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_0 h_2
gene expression data of hN2 human neural cells not exposed to VX at the beginning of experiment
|
Sample_geo_accession | GSM819946
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819946/suppl/GSM819946_hN2_0_uM_0_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819947 | GPL570 |
|
hN2_control_0 h_rep3
|
hN2 human neural cells not exposed to VX at the beginning of experiment
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_0 h_3
gene expression data of hN2 human neural cells not exposed to VX at the beginning of experiment
|
Sample_geo_accession | GSM819947
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819947/suppl/GSM819947_hN2_0_uM_0_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819948 | GPL570 |
|
hN2_control_0 h_rep4
|
hN2 human neural cells not exposed to VX at the beginning of experiment
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_0 h_4
gene expression data of hN2 human neural cells not exposed to VX at the beginning of experiment
|
Sample_geo_accession | GSM819948
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819948/suppl/GSM819948_hN2_0_uM_0_h_4.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819949 | GPL570 |
|
hN2_control_6 h_rep1
|
hN2 human neural cells not exposed to VX at 6 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_6 h_1
gene expression data of hN2 human neural cells not exposed to VX at 6 h
|
Sample_geo_accession | GSM819949
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819949/suppl/GSM819949_hN2_0_uM_6_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819950 | GPL570 |
|
hN2_control_6 h_rep2
|
hN2 human neural cells not exposed to VX at 6 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_6 h_2
gene expression data of hN2 human neural cells not exposed to VX at 6 h
|
Sample_geo_accession | GSM819950
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819950/suppl/GSM819950_hN2_0_uM_6_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819951 | GPL570 |
|
hN2_control_6 h_rep3
|
hN2 human neural cells not exposed to VX at 6 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_6 h_3
gene expression data of hN2 human neural cells not exposed to VX at 6 h
|
Sample_geo_accession | GSM819951
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819951/suppl/GSM819951_hN2_0_uM_6_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819952 | GPL570 |
|
hN2_control_24 h_rep1
|
hN2 human neural cells not exposed to VX at 24 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_24 h_1
gene expression data of hN2 human neural cells not exposed to VX at 24 h
|
Sample_geo_accession | GSM819952
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819952/suppl/GSM819952_hN2_0_uM_24_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819953 | GPL570 |
|
hN2_control_24 h_rep2
|
hN2 human neural cells not exposed to VX at 24 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_24 h_2
gene expression data of hN2 human neural cells not exposed to VX at 24 h
|
Sample_geo_accession | GSM819953
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819953/suppl/GSM819953_hN2_0_uM_24_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819954 | GPL570 |
|
hN2_control_24 h_rep3
|
hN2 human neural cells not exposed to VX at 24 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_24 h_3
gene expression data of hN2 human neural cells not exposed to VX at 24 h
|
Sample_geo_accession | GSM819954
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819954/suppl/GSM819954_hN2_0_uM_24_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819955 | GPL570 |
|
hN2_control_24 h_rep4
|
hN2 human neural cells not exposed to VX at 24 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_24 h_4
gene expression data of hN2 human neural cells not exposed to VX at 24 h
|
Sample_geo_accession | GSM819955
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819955/suppl/GSM819955_hN2_0_uM_24_h_4.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819956 | GPL570 |
|
hN2_control_72 h_rep1
|
hN2 human neural cells not exposed to VX at 72 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_72 h_1
gene expression data of hN2 human neural cells not exposed to VX at 72 h
|
Sample_geo_accession | GSM819956
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819956/suppl/GSM819956_hN2_0_uM_72_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819957 | GPL570 |
|
hN2_control_72 h_rep2
|
hN2 human neural cells not exposed to VX at 72 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_72 h_2
gene expression data of hN2 human neural cells not exposed to VX at 72 h
|
Sample_geo_accession | GSM819957
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819957/suppl/GSM819957_hN2_0_uM_72_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819958 | GPL570 |
|
hN2_control_72 h_rep3
|
hN2 human neural cells not exposed to VX at 72 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_72 h_3
gene expression data of hN2 human neural cells not exposed to VX at 72 h
|
Sample_geo_accession | GSM819958
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819958/suppl/GSM819958_hN2_0_uM_72_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819959 | GPL570 |
|
hN2_control_72 h_rep4
|
hN2 human neural cells not exposed to VX at 72 h
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0 μM_72 h_4
gene expression data of hN2 human neural cells not exposed to VX at 72 h
|
Sample_geo_accession | GSM819959
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819959/suppl/GSM819959_hN2_0_uM_72_h_4.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819960 | GPL570 |
|
hN2_0.1 μM_6 h_1
|
hN2 human neural cells exposed to 0.1 μM VX 6 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_6 h_1
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 6 h after exposure
|
Sample_geo_accession | GSM819960
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819960/suppl/GSM819960_hN2_0.1_uM_6_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819961 | GPL570 |
|
hN2_0.1 μM_6 h_2
|
hN2 human neural cells exposed to 0.1 μM VX 6 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_6 h_2
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 6 h after exposure
|
Sample_geo_accession | GSM819961
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819961/suppl/GSM819961_hN2_0.1_uM_6_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819962 | GPL570 |
|
hN2_0.1 μM_6 h_3
|
hN2 human neural cells exposed to 0.1 μM VX 6 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_6 h_3
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 6 h after exposure
|
Sample_geo_accession | GSM819962
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819962/suppl/GSM819962_hN2_0.1_uM_6_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819963 | GPL570 |
|
hN2_0.1 μM_6 h_4
|
hN2 human neural cells exposed to 0.1 μM VX 6 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_6 h_4
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 6 h after exposure
|
Sample_geo_accession | GSM819963
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819963/suppl/GSM819963_hN2_0.1_uM_6_h_4.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819964 | GPL570 |
|
hN2_0.1 μM_24 h_1
|
hN2 human neural cells exposed to 0.1 μM VX 24 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_24 h_1
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 24 h after exposure
|
Sample_geo_accession | GSM819964
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819964/suppl/GSM819964_hN2_0.1_uM_24_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819965 | GPL570 |
|
hN2_0.1 μM_24 h_2
|
hN2 human neural cells exposed to 0.1 μM VX 24 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_24 h_2
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 24 h after exposure
|
Sample_geo_accession | GSM819965
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819965/suppl/GSM819965_hN2_0.1_uM_24_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819966 | GPL570 |
|
hN2_0.1 μM_24 h_3
|
hN2 human neural cells exposed to 0.1 μM VX 24 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_24 h_3
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 24 h after exposure
|
Sample_geo_accession | GSM819966
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819966/suppl/GSM819966_hN2_0.1_uM_24_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819967 | GPL570 |
|
hN2_0.1 μM_24 h_4
|
hN2 human neural cells exposed to 0.1 μM VX 24 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_24 h_4
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 24 h after exposure
|
Sample_geo_accession | GSM819967
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819967/suppl/GSM819967_hN2_0.1_uM_24_h_4.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819968 | GPL570 |
|
hN2_0.1 μM_72 h_1
|
hN2 human neural cells exposed to 0.1 μM VX 72 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_72 h_1
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 72 h after exposure
|
Sample_geo_accession | GSM819968
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819968/suppl/GSM819968_hN2_0.1_uM_72_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819969 | GPL570 |
|
hN2_0.1 μM_72 h_2
|
hN2 human neural cells exposed to 0.1 μM VX 72 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_72 h_2
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 72 h after exposure
|
Sample_geo_accession | GSM819969
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819969/suppl/GSM819969_hN2_0.1_uM_72_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819970 | GPL570 |
|
hN2_0.1 μM_72 h_3
|
hN2 human neural cells exposed to 0.1 μM VX 72 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_72 h_3
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 72 h after exposure
|
Sample_geo_accession | GSM819970
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819970/suppl/GSM819970_hN2_0.1_uM_72_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819971 | GPL570 |
|
hN2_0.1 μM_72 h_4
|
hN2 human neural cells exposed to 0.1 μM VX 72 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_0.1 μM_72 h_4
gene expression data of hN2 human neural cells exposed to 0.1 μM VX 72 h after exposure
|
Sample_geo_accession | GSM819971
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819971/suppl/GSM819971_hN2_0.1_uM_72_h_4.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819972 | GPL570 |
|
hN2_10 μM_6 h_1
|
hN2 human neural cells exposed to 10 μM VX 6 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_6 h_1
gene expression data of hN2 human neural cells exposed to 10 μM VX 6 h after exposure
|
Sample_geo_accession | GSM819972
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819972/suppl/GSM819972_hN2_10_uM_6_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819973 | GPL570 |
|
hN2_10 μM_6 h_2
|
hN2 human neural cells exposed to 10 μM VX 6 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_6 h_2
gene expression data of hN2 human neural cells exposed to 10 μM VX 6 h after exposure
|
Sample_geo_accession | GSM819973
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819973/suppl/GSM819973_hN2_10_uM_6_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819974 | GPL570 |
|
hN2_10 μM_6 h_3
|
hN2 human neural cells exposed to 10 μM VX 6 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_6 h_3
gene expression data of hN2 human neural cells exposed to 10 μM VX 6 h after exposure
|
Sample_geo_accession | GSM819974
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819974/suppl/GSM819974_hN2_10_uM_6_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819975 | GPL570 |
|
hN2_10 μM_6 h_4
|
hN2 human neural cells exposed to 10 μM VX 6 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_6 h_4
gene expression data of hN2 human neural cells exposed to 10 μM VX 6 h after exposure
|
Sample_geo_accession | GSM819975
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819975/suppl/GSM819975_hN2_10_uM_6_h_4.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819976 | GPL570 |
|
hN2_10 μM_24 h_1
|
hN2 human neural cells exposed to 10 μM VX 24 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_24 h_1
gene expression data of hN2 human neural cells exposed to 10 μM VX 24 h after exposure
|
Sample_geo_accession | GSM819976
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819976/suppl/GSM819976_hN2_10_uM_24_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819977 | GPL570 |
|
hN2_10 μM_24 h_2
|
hN2 human neural cells exposed to 10 μM VX 24 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_24 h_2
gene expression data of hN2 human neural cells exposed to 10 μM VX 24 h after exposure
|
Sample_geo_accession | GSM819977
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819977/suppl/GSM819977_hN2_10_uM_24_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819978 | GPL570 |
|
hN2_10 μM_24 h_3
|
hN2 human neural cells exposed to 10 μM VX 24 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_24 h_3
gene expression data of hN2 human neural cells exposed to 10 μM VX 24 h after exposure
|
Sample_geo_accession | GSM819978
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819978/suppl/GSM819978_hN2_10_uM_24_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819979 | GPL570 |
|
hN2_10 μM_24 h_4
|
hN2 human neural cells exposed to 10 μM VX 24 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_24 h_4
gene expression data of hN2 human neural cells exposed to 10 μM VX 24 h after exposure
|
Sample_geo_accession | GSM819979
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819979/suppl/GSM819979_hN2_10_uM_24_h_4.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819980 | GPL570 |
|
hN2_10 μM_72 h_1
|
hN2 human neural cells exposed to 10 μM VX 72 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_72 h_1
gene expression data of hN2 human neural cells exposed to 10 μM VX 72 h after exposure
|
Sample_geo_accession | GSM819980
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819980/suppl/GSM819980_hN2_10_uM_72_h_1.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819981 | GPL570 |
|
hN2_10 μM_72 h_2
|
hN2 human neural cells exposed to 10 μM VX 72 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_72 h_2
gene expression data of hN2 human neural cells exposed to 10 μM VX 72 h after exposure
|
Sample_geo_accession | GSM819981
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819981/suppl/GSM819981_hN2_10_uM_72_h_2.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819982 | GPL570 |
|
hN2_10 μM_72 h_3
|
hN2 human neural cells exposed to 10 μM VX 72 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_72 h_3
gene expression data of hN2 human neural cells exposed to 10 μM VX 72 h after exposure
|
Sample_geo_accession | GSM819982
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819982/suppl/GSM819982_hN2_10_uM_72_h_3.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
GSM819983 | GPL570 |
|
hN2_10 μM_72 h_4
|
hN2 human neural cells exposed to 10 μM VX 72 h after exposure
|
cell type: hN2 neural cells derived from WA09 human embryonic stem cells
|
hN2_10 μM_72 h_4
gene expression data of hN2 human neural cells exposed to 10 μM VX 72 h after exposure
|
Sample_geo_accession | GSM819983
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | Oct 20 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| Sample_growth_protocol_ch1 | Human hN2 neural cells were seeded in 12-well plates in media formulated by the manufacturer (Neuromics, Edina, MN) and cultured at 37 °C under humidified 5% CO2 for 48 h (without changing media) before VX exposure.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3' IVT Express Kit following the manufacturer's protocol.
| Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer's protocols.
| Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Xiugong,,Gao
| Sample_contact_email | xiugong.gao@amedd.army.mil
| Sample_contact_institute | Walter Reed Army Institute of Research
| Sample_contact_address | 503 Robert Grant Ave
| Sample_contact_city | Silver Spring
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20910
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM819nnn/GSM819983/suppl/GSM819983_hN2_10_uM_72_h_4.CEL.gz
| Sample_series_id | GSE33109
| Sample_data_row_count | 54675
| |
|
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