Search results for the GEO ID: GSE33121  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM820417 | GPL1261 |
|
undifferentiated cKit+ SSEA+ ESC rep 1
|
V6.5 ESC
|
cell type: undifferentiated cKit+ SSEA+ ESC
genetic background: 129xBL6 F1
|
Gene expression of undifferentiated ESCs
|
| Sample_geo_accession | GSM820417
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820417/suppl/GSM820417_Ckit_SSEA_++_V6.5_A_RNA.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820417/suppl/GSM820417_Ckit_SSEA_++_V6.5_A_RNA.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
|
GSM820418 | GPL1261 |
|
undifferentiated cKit+ SSEA+ ESC rep 2
|
V6.5 ESC
|
cell type: undifferentiated cKit+ SSEA+ ESC
genetic background: 129xBL6 F1
|
Gene expression of undifferentiated ESCs
|
| Sample_geo_accession | GSM820418
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820418/suppl/GSM820418_Ckit_SSEA_++_V6.5_B_RNA.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820418/suppl/GSM820418_Ckit_SSEA_++_V6.5_B_RNA.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
|
GSM820419 | GPL1261 |
|
cKit+ SSEA+ iPGC rep 1
|
V6.5 derived PGC
|
cell type: in vitro cKit+ SSEA+ iPGC
genetic background: 129xBL6 F1
|
Gene expression of in vitro PGC
|
| Sample_geo_accession | GSM820419
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820419/suppl/GSM820419_Ssea1_ckit_++_5.22.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820419/suppl/GSM820419_Ssea1_ckit_++_5.22.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
|
GSM820420 | GPL1261 |
|
cKit+ SSEA+ iPGC rep 2
|
V6.5 derived PGC
|
cell type: in vitro cKit+ SSEA+ iPGC
genetic background: 129xBL6 F1
|
Gene expression of in vitro PGC
|
| Sample_geo_accession | GSM820420
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820420/suppl/GSM820420_Ssea1_ckit_++_5.28.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820420/suppl/GSM820420_Ssea1_ckit_++_5.28.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
|
GSM820421 | GPL1261 |
|
cKit+ Oct4+ iPGC rep 1
|
Oct4-gfp derived PGC
|
cell type: in vitro cKit+ Oct4+ iPGC
genetic background: mixed
|
Gene expression of in vitro PGC
|
| Sample_geo_accession | GSM820421
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820421/suppl/GSM820421_Ckit_oct4_++_5.15.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820421/suppl/GSM820421_Ckit_oct4_++_5.15.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
|
GSM820422 | GPL1261 |
|
cKit+ Oct4+ iPGC rep 2
|
Oct4-gfp derived PGC
|
cell type: in vitro cKit+ Oct4+ iPGC
genetic background: mixed
|
Gene expression of in vitro PGC
|
| Sample_geo_accession | GSM820422
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820422/suppl/GSM820422_Ckit_oct4_++_5.19.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820422/suppl/GSM820422_Ckit_oct4_++_5.19.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
|
GSM820423 | GPL1261 |
|
cKit- SSEA- somatic rep 1
|
V6.5 derived somatic
|
cell type: in vitro cKit- SSEA- somatic
genetic background: 129xBL6 F1
|
Gene expression of in vitro somatic cells
|
| Sample_geo_accession | GSM820423
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820423/suppl/GSM820423_Ssea1_ckit_--_5.22.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820423/suppl/GSM820423_Ssea1_ckit_--_5.22.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
|
GSM820424 | GPL1261 |
|
cKit- SSEA- somatic rep 2
|
V6.5 derived somatic
|
cell type: in vitro cKit- SSEA- somatic
genetic background: 129xBL6 F1
|
Gene expression of in vitro somatic cells
|
| Sample_geo_accession | GSM820424
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820424/suppl/GSM820424_Ssea1_ckit_--_5.28.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820424/suppl/GSM820424_Ssea1_ckit_--_5.28.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
|
GSM820425 | GPL1261 |
|
cKit- Oct4- somatic rep 1
|
Oct4-gfp derived somatic
|
cell type: in vitro cKit- Oct4- somatic
genetic background: mixed
|
Gene expression of in vitro somatic cells
|
| Sample_geo_accession | GSM820425
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820425/suppl/GSM820425_Ckit_oct4_--_5.15.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820425/suppl/GSM820425_Ckit_oct4_--_5.15.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
|
GSM820426 | GPL1261 |
|
cKit- Oct4- somatic rep 2
|
Oct4-gfp derived somatic
|
cell type: intro cKit- Oct4- somatic
genetic background: mixed
|
Gene expression of in vitro somatic cells
|
| Sample_geo_accession | GSM820426
| | Sample_status | Public on Dec 15 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Dec 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
| | Sample_growth_protocol_ch1 | Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
| | Sample_data_processing | The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | John,,Vincent
| | Sample_contact_email | jvincent@ucla.edu
| | Sample_contact_department | MCDB
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 621 Charles E Young Dr. S
| | Sample_contact_city | Los Angeles
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820426/suppl/GSM820426_Ckit_oct4_--_5.19.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820426/suppl/GSM820426_Ckit_oct4_--_5.19.mas5.CHP.gz
| | Sample_series_id | GSE33121
| | Sample_data_row_count | 45101
| | |
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