Search results for the GEO ID: GSE33132  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM820578 | GPL1261 |
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Round spermatids_BrdtΔBD1 mutant _purified_ rep1
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Purified BrdtΔBD1 mutant round spermatids
|
tissue: Purified round spermatids from BrdtΔBD1 mutant
strain: 129 Sv/Ev
age: Adults, 2-4 months old
|
Gene expression data from mutant round spermatids
|
| Sample_geo_accession | GSM820578
| | Sample_status | Public on Oct 21 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Oct 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pure populations of round spermatids were separated on a 4%-2% BSA in DPBS gradient.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. This RNA was then purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Signal intensities were ascertained and statistical analysis was performed within the R\Bioconductor statistical framework . We used the limma package to pre-process the raw data and perform the quality controls. Expression intensities were background corrected, normalized, and summarized using the Gene Chip Robust Multiarray Algorithm (GC-RMA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Binyamin,David,Berkovits
| | Sample_contact_laboratory | Wolgemuth
| | Sample_contact_department | Genetics and Development
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave. Room 611
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820578/suppl/GSM820578.CEL.gz
| | Sample_series_id | GSE33132
| | Sample_data_row_count | 45101
| | |
|
GSM820579 | GPL1261 |
|
Round spermatids_BrdtΔBD1 mutant _purified_ rep2
|
Purified BrdtΔBD1 mutant round spermatids
|
tissue: Purified round spermatids from BrdtΔBD1 mutant
strain: 129 Sv/Ev
age: Adults, 2-4 months old
|
Gene expression data from mutant round spermatids
|
| Sample_geo_accession | GSM820579
| | Sample_status | Public on Oct 21 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Oct 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pure populations of round spermatids were separated on a 4%-2% BSA in DPBS gradient.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. This RNA was then purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Signal intensities were ascertained and statistical analysis was performed within the R\Bioconductor statistical framework . We used the limma package to pre-process the raw data and perform the quality controls. Expression intensities were background corrected, normalized, and summarized using the Gene Chip Robust Multiarray Algorithm (GC-RMA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Binyamin,David,Berkovits
| | Sample_contact_laboratory | Wolgemuth
| | Sample_contact_department | Genetics and Development
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave. Room 611
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820579/suppl/GSM820579.CEL.gz
| | Sample_series_id | GSE33132
| | Sample_data_row_count | 45101
| | |
|
GSM820580 | GPL1261 |
|
Round spermatids_BrdtΔBD1 mutant _purified_ rep3
|
Purified BrdtΔBD1 mutant round spermatids
|
tissue: Purified round spermatids from BrdtΔBD1 mutant
strain: 129 Sv/Ev
age: Adults, 2-4 months old
|
Gene expression data from mutant round spermatids
|
| Sample_geo_accession | GSM820580
| | Sample_status | Public on Oct 21 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Oct 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pure populations of round spermatids were separated on a 4%-2% BSA in DPBS gradient.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. This RNA was then purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Signal intensities were ascertained and statistical analysis was performed within the R\Bioconductor statistical framework . We used the limma package to pre-process the raw data and perform the quality controls. Expression intensities were background corrected, normalized, and summarized using the Gene Chip Robust Multiarray Algorithm (GC-RMA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Binyamin,David,Berkovits
| | Sample_contact_laboratory | Wolgemuth
| | Sample_contact_department | Genetics and Development
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave. Room 611
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820580/suppl/GSM820580.CEL.gz
| | Sample_series_id | GSE33132
| | Sample_data_row_count | 45101
| | |
|
GSM820581 | GPL1261 |
|
Round spermatids_BrdtΔBD1 mutant _purified_ rep4
|
Purified BrdtΔBD1 mutant round spermatids
|
tissue: Purified round spermatids from BrdtΔBD1 mutant
strain: 129 Sv/Ev
age: Adults, 2-4 months old
|
Gene expression data from mutant round spermatids
|
| Sample_geo_accession | GSM820581
| | Sample_status | Public on Oct 21 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Oct 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pure populations of round spermatids were separated on a 4%-2% BSA in DPBS gradient.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. This RNA was then purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Signal intensities were ascertained and statistical analysis was performed within the R\Bioconductor statistical framework . We used the limma package to pre-process the raw data and perform the quality controls. Expression intensities were background corrected, normalized, and summarized using the Gene Chip Robust Multiarray Algorithm (GC-RMA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Binyamin,David,Berkovits
| | Sample_contact_laboratory | Wolgemuth
| | Sample_contact_department | Genetics and Development
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave. Room 611
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820581/suppl/GSM820581.CEL.gz
| | Sample_series_id | GSE33132
| | Sample_data_row_count | 45101
| | |
|
GSM820582 | GPL1261 |
|
Round spermatis_control_purified_rep1
|
Purified control round spermatids
|
tissue: Purified round spermatids
strain: 129 Sv/Ev
age: Adults, 2-4 months old
|
Gene expression data from control round spermatids
|
| Sample_geo_accession | GSM820582
| | Sample_status | Public on Oct 21 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Oct 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pure populations of round spermatids were separated on a 4%-2% BSA in DPBS gradient.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. This RNA was then purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Signal intensities were ascertained and statistical analysis was performed within the R\Bioconductor statistical framework . We used the limma package to pre-process the raw data and perform the quality controls. Expression intensities were background corrected, normalized, and summarized using the Gene Chip Robust Multiarray Algorithm (GC-RMA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Binyamin,David,Berkovits
| | Sample_contact_laboratory | Wolgemuth
| | Sample_contact_department | Genetics and Development
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave. Room 611
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820582/suppl/GSM820582.CEL.gz
| | Sample_series_id | GSE33132
| | Sample_data_row_count | 45101
| | |
|
GSM820583 | GPL1261 |
|
Round spermatis_control_purified_rep2
|
Purified control round spermatids
|
tissue: Purified round spermatids
strain: 129 Sv/Ev
age: Adults, 2-4 months old
|
Gene expression data from control round spermatids
|
| Sample_geo_accession | GSM820583
| | Sample_status | Public on Oct 21 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Oct 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pure populations of round spermatids were separated on a 4%-2% BSA in DPBS gradient.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. This RNA was then purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Signal intensities were ascertained and statistical analysis was performed within the R\Bioconductor statistical framework . We used the limma package to pre-process the raw data and perform the quality controls. Expression intensities were background corrected, normalized, and summarized using the Gene Chip Robust Multiarray Algorithm (GC-RMA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Binyamin,David,Berkovits
| | Sample_contact_laboratory | Wolgemuth
| | Sample_contact_department | Genetics and Development
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave. Room 611
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820583/suppl/GSM820583.CEL.gz
| | Sample_series_id | GSE33132
| | Sample_data_row_count | 45101
| | |
|
GSM820584 | GPL1261 |
|
Round spermatis_control_purified_rep3
|
Purified control round spermatids
|
tissue: Purified round spermatids
strain: 129 Sv/Ev
age: Adults, 2-4 months old
|
Gene expression data from control round spermatids
|
| Sample_geo_accession | GSM820584
| | Sample_status | Public on Oct 21 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Oct 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pure populations of round spermatids were separated on a 4%-2% BSA in DPBS gradient.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. This RNA was then purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Signal intensities were ascertained and statistical analysis was performed within the R\Bioconductor statistical framework . We used the limma package to pre-process the raw data and perform the quality controls. Expression intensities were background corrected, normalized, and summarized using the Gene Chip Robust Multiarray Algorithm (GC-RMA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Binyamin,David,Berkovits
| | Sample_contact_laboratory | Wolgemuth
| | Sample_contact_department | Genetics and Development
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave. Room 611
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820584/suppl/GSM820584.CEL.gz
| | Sample_series_id | GSE33132
| | Sample_data_row_count | 45101
| | |
|
GSM820585 | GPL1261 |
|
Round spermatis_control_purified_rep4
|
Purified control round spermatids
|
tissue: Purified round spermatids
strain: 129 Sv/Ev
age: Adults, 2-4 months old
|
Gene expression data from control round spermatids
|
| Sample_geo_accession | GSM820585
| | Sample_status | Public on Oct 21 2011
| | Sample_submission_date | Oct 20 2011
| | Sample_last_update_date | Oct 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pure populations of round spermatids were separated on a 4%-2% BSA in DPBS gradient.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. This RNA was then purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Signal intensities were ascertained and statistical analysis was performed within the R\Bioconductor statistical framework . We used the limma package to pre-process the raw data and perform the quality controls. Expression intensities were background corrected, normalized, and summarized using the Gene Chip Robust Multiarray Algorithm (GC-RMA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Binyamin,David,Berkovits
| | Sample_contact_laboratory | Wolgemuth
| | Sample_contact_department | Genetics and Development
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave. Room 611
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820585/suppl/GSM820585.CEL.gz
| | Sample_series_id | GSE33132
| | Sample_data_row_count | 45101
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