Search results for the GEO ID: GSE33167  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM820808 | GPL570 |
|
HMEC rep1
|
HMEC cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line HMEC
culture medium: MEGM
|
HMEC cells cultured in MEGM (Lonza)
HGU133(+2.0)_01_3A.CEL
|
| Sample_geo_accession | GSM820808
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_growth_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820808/suppl/GSM820808.CEL.gz
| | Sample_series_id | GSE33145
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 11462
| | |
|
GSM820809 | GPL570 |
|
HMEC rep2
|
HMEC cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line HMEC
culture medium: MEGM
|
HMEC cells cultured in MEGM (Lonza)
HGU133(+2.0)_07_3B.CEL
|
| Sample_geo_accession | GSM820809
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_growth_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820809/suppl/GSM820809.CEL.gz
| | Sample_series_id | GSE33145
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 11462
| | |
|
GSM820810 | GPL570 |
|
HMEC rep3
|
HMEC cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line HMEC
culture medium: MEGM
|
HMEC cells cultured in MEGM (Lonza)
HGU133(+2.0)_13_3C.CEL
|
| Sample_geo_accession | GSM820810
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_growth_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820810/suppl/GSM820810.CEL.gz
| | Sample_series_id | GSE33145
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 11462
| | |
|
GSM820811 | GPL570 |
|
DKAT rep1
|
DKAT cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line DKAT
culture medium: MEGM
|
DKAT cells cultured in MEGM (Lonza)
HGU133(+2.0)_03_5A.CEL
|
| Sample_geo_accession | GSM820811
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_growth_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820811/suppl/GSM820811.CEL.gz
| | Sample_series_id | GSE33145
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 11462
| | |
|
GSM820812 | GPL570 |
|
DKAT rep2
|
DKAT cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line DKAT
culture medium: MEGM
|
DKAT cells cultured in MEGM (Lonza)
HGU133(+2.0)_09_5B.CEL
|
| Sample_geo_accession | GSM820812
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_growth_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820812/suppl/GSM820812.CEL.gz
| | Sample_series_id | GSE33145
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 11462
| | |
|
GSM820813 | GPL570 |
|
DKAT rep3
|
DKAT cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line DKAT
culture medium: MEGM
|
DKAT cells cultured in MEGM (Lonza)
HGU133(+2.0)_15_5C.CEL
|
| Sample_geo_accession | GSM820813
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_growth_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820813/suppl/GSM820813.CEL.gz
| | Sample_series_id | GSE33145
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 11462
| | |
|
GSM820814 | GPL570 |
|
MDA-MB-231 rep1
|
MDA-MB-231 cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line MDA-MB-231
culture medium: MEGM
|
MDA-MB-231 cells cultured in MEGM (Lonza)
HGU133(+2.0)_04_6A.CEL
|
| Sample_geo_accession | GSM820814
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_growth_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820814/suppl/GSM820814.CEL.gz
| | Sample_series_id | GSE33145
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 11462
| | |
|
GSM820815 | GPL570 |
|
MDA-MB-231 rep2
|
MDA-MB-231 cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line MDA-MB-231
culture medium: MEGM
|
MDA-MB-231 cells cultured in MEGM (Lonza)
HGU133(+2.0)_10_6B.CEL
|
| Sample_geo_accession | GSM820815
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_growth_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820815/suppl/GSM820815.CEL.gz
| | Sample_series_id | GSE33145
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 11462
| | |
|
GSM820816 | GPL570 |
|
MDA-MB-231 rep3
|
MDA-MB-231 cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line MDA-MB-231
culture medium: MEGM
|
MDA-MB-231 cells cultured in MEGM (Lonza)
HGU133(+2.0)_16_6C.CEL
|
| Sample_geo_accession | GSM820816
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_growth_protocol_ch1 | DKAT, HMEC, or MDA-MB-231 cells were cultured in MEGM (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820816/suppl/GSM820816.CEL.gz
| | Sample_series_id | GSE33145
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 11462
| | |
|
GSM820817 | GPL570 |
|
DKAT MEGM rep1
|
DKAT cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line DKAT
culture medium: MEGM
|
DKAT cells cultured in MEGM (Lonza)
HGU133(+2.0)_04_D1.CEL
|
| Sample_geo_accession | GSM820817
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_growth_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820817/suppl/GSM820817.CEL.gz
| | Sample_series_id | GSE33146
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 54675
| | |
|
GSM820818 | GPL570 |
|
DKAT MEGM rep2
|
DKAT cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line DKAT
culture medium: MEGM
|
DKAT cells cultured in MEGM (Lonza)
HGU133(+2.0)_13_D2.CEL
|
| Sample_geo_accession | GSM820818
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_growth_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820818/suppl/GSM820818.CEL.gz
| | Sample_series_id | GSE33146
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 54675
| | |
|
GSM820819 | GPL570 |
|
DKAT MEGM rep3
|
DKAT cells cultured in MEGM (Lonza)
|
cell line: Human breast cancer-derived cell line DKAT
culture medium: MEGM
|
DKAT cells cultured in MEGM (Lonza)
HGU133(+2.0)_22_D3.CEL
|
| Sample_geo_accession | GSM820819
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_growth_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820819/suppl/GSM820819.CEL.gz
| | Sample_series_id | GSE33146
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 54675
| | |
|
GSM820820 | GPL570 |
|
DKAT SCGM day14 rep1
|
DKAT cells cultured in SCGM (Lonza) for 14 days
|
cell line: Human breast cancer-derived cell line DKAT
culture medium: SCGM
|
DKAT cells cultured in SCGM (Lonza) for 14 days
HGU133(+2.0)_05_E1.CEL
|
| Sample_geo_accession | GSM820820
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_growth_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820820/suppl/GSM820820.CEL.gz
| | Sample_series_id | GSE33146
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 54675
| | |
|
GSM820821 | GPL570 |
|
DKAT SCGM day14 rep2
|
DKAT cells cultured in SCGM (Lonza) for 14 days
|
cell line: Human breast cancer-derived cell line DKAT
culture medium: SCGM
|
DKAT cells cultured in SCGM (Lonza) for 14 days
HGU133(+2.0)_14_E2.CEL
|
| Sample_geo_accession | GSM820821
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_growth_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820821/suppl/GSM820821.CEL.gz
| | Sample_series_id | GSE33146
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 54675
| | |
|
GSM820822 | GPL570 |
|
DKAT SCGM day14 rep3
|
DKAT cells cultured in SCGM (Lonza) for 14 days
|
cell line: Human breast cancer-derived cell line DKAT
culture medium: SCGM
|
DKAT cells cultured in SCGM (Lonza) for 14 days
HGU133(+2.0)_23_E3.CEL
|
| Sample_geo_accession | GSM820822
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Oct 21 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_growth_protocol_ch1 | DKAT cells (passage 10) in MEGM were split and grown for 14 days either in MEGM (Lonza) or SCGM (Lonza) in adherent culture.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen RNeasy kit according to the manufacturer's instructions with option DNAse step.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized in an Affymetrix Hybridization Oven 640 for 16 hr at 45°C on HG-U133 Plus2.0 GeneChips. GeneChips were washed and stained on an Affymetrix Fluidics Station 450, using the manufacture’s recommended protocol.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with RMA using the affy package implemented in BRB array tools (Simon and Lam, ver. 3.7.0; R ver. 2.6.0)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicholas,,D'Amato
| | Sample_contact_laboratory | Seewaldt
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University
| | Sample_contact_address | MSRB Room 217, Research Dr.
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM820nnn/GSM820822/suppl/GSM820822.CEL.gz
| | Sample_series_id | GSE33146
| | Sample_series_id | GSE33167
| | Sample_data_row_count | 54675
| | |
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