Search results for the GEO ID: GSE33325  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM824086 | GPL570 |
|
iCell_control_0 h_rep1
|
iCell human cardiomyocytes not exposed to VX at the beginning of experiment
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_0h_1
gene expression data of iCell human cardiomyocytes not exposed to VX at the beginning of experiment
|
| Sample_geo_accession | GSM824086
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824086/suppl/GSM824086_iCell_0_uM_0h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824087 | GPL570 |
|
iCell_control_0 h_rep2
|
iCell human cardiomyocytes not exposed to VX at the beginning of experiment
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_0h_2
gene expression data of iCell human cardiomyocytes not exposed to VX at the beginning of experiment
|
| Sample_geo_accession | GSM824087
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824087/suppl/GSM824087_iCell_0_uM_0h_2.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824088 | GPL570 |
|
iCell_control_0 h_rep3
|
iCell human cardiomyocytes not exposed to VX at the beginning of experiment
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_0h_3
gene expression data of iCell human cardiomyocytes not exposed to VX at the beginning of experiment
|
| Sample_geo_accession | GSM824088
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824088/suppl/GSM824088_iCell_0_uM_0h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824089 | GPL570 |
|
iCell_control_0 h_rep4
|
iCell human cardiomyocytes not exposed to VX at the beginning of experiment
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_0h_4
gene expression data of iCell human cardiomyocytes not exposed to VX at the beginning of experiment
|
| Sample_geo_accession | GSM824089
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824089/suppl/GSM824089_iCell_0_uM_0h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824090 | GPL570 |
|
iCell_control_6 h_rep1
|
iCell human cardiomyocytes not exposed to VX at 6 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_6h_1
gene expression data of iCell human cardiomyocytes not exposed to VX at 6 h
|
| Sample_geo_accession | GSM824090
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824090/suppl/GSM824090_iCell_0_uM_6h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824091 | GPL570 |
|
iCell_control_6 h_rep2
|
iCell human cardiomyocytes not exposed to VX at 6 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_6h_2
gene expression data of iCell human cardiomyocytes not exposed to VX at 6 h
|
| Sample_geo_accession | GSM824091
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824091/suppl/GSM824091_iCell_0_uM_6h_2.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824092 | GPL570 |
|
iCell_control_6 h_rep3
|
iCell human cardiomyocytes not exposed to VX at 6 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_6h_3
gene expression data of iCell human cardiomyocytes not exposed to VX at 6 h
|
| Sample_geo_accession | GSM824092
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824092/suppl/GSM824092_iCell_0_uM_6h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824093 | GPL570 |
|
iCell_control_6 h_rep4
|
iCell human cardiomyocytes not exposed to VX at 6 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_6h_4
gene expression data of iCell human cardiomyocytes not exposed to VX at 6 h
|
| Sample_geo_accession | GSM824093
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824093/suppl/GSM824093_iCell_0_uM_6h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824094 | GPL570 |
|
iCell_control_24 h_rep1
|
iCell human cardiomyocytes not exposed to VX at 24 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_24h_1
gene expression data of iCell human cardiomyocytes not exposed to VX at 24 h
|
| Sample_geo_accession | GSM824094
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824094/suppl/GSM824094_iCell_0_uM_24h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824095 | GPL570 |
|
iCell_control_24 h_rep2
|
iCell human cardiomyocytes not exposed to VX at 24 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_24h_2
gene expression data of iCell human cardiomyocytes not exposed to VX at 24 h
|
| Sample_geo_accession | GSM824095
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824095/suppl/GSM824095_iCell_0_uM_24h_2.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824096 | GPL570 |
|
iCell_control_24 h_rep3
|
iCell human cardiomyocytes not exposed to VX at 24 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_24h_3
gene expression data of iCell human cardiomyocytes not exposed to VX at 24 h
|
| Sample_geo_accession | GSM824096
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824096/suppl/GSM824096_iCell_0_uM_24h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824097 | GPL570 |
|
iCell_control_24 h_rep4
|
iCell human cardiomyocytes not exposed to VX at 24 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_24h_4
gene expression data of iCell human cardiomyocytes not exposed to VX at 24 h
|
| Sample_geo_accession | GSM824097
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824097/suppl/GSM824097_iCell_0_uM_24h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824098 | GPL570 |
|
iCell_control_72 h_rep1
|
iCell human cardiomyocytes not exposed to VX at 72 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_72h_1
gene expression data of iCell human cardiomyocytes not exposed to VX at 72 h
|
| Sample_geo_accession | GSM824098
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824098/suppl/GSM824098_iCell_0_uM_72h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824099 | GPL570 |
|
iCell_control_72 h_rep2
|
iCell human cardiomyocytes not exposed to VX at 72 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_72h_2
gene expression data of iCell human cardiomyocytes not exposed to VX at 72 h
|
| Sample_geo_accession | GSM824099
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824099/suppl/GSM824099_iCell_0_uM_72h_2.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824100 | GPL570 |
|
iCell_control_72 h_rep3
|
iCell human cardiomyocytes not exposed to VX at 72 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_72h_3
gene expression data of iCell human cardiomyocytes not exposed to VX at 72 h
|
| Sample_geo_accession | GSM824100
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824100/suppl/GSM824100_iCell_0_uM_72h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824101 | GPL570 |
|
iCell_control_72 h_rep4
|
iCell human cardiomyocytes not exposed to VX at 72 h
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0 uM_72h_4
gene expression data of iCell human cardiomyocytes not exposed to VX at 72 h
|
| Sample_geo_accession | GSM824101
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824101/suppl/GSM824101_iCell_0_uM_72h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824102 | GPL570 |
|
iCell_0.1 μM_6 h_rep1
|
iCell human cardiomyocytes exposed to 0.1 μM VX 6 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_6h_1
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM824102
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824102/suppl/GSM824102_iCell_0.1_uM_6h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824103 | GPL570 |
|
iCell_0.1 μM_6 h_rep2
|
iCell human cardiomyocytes exposed to 0.1 μM VX 6 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_6h_2
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM824103
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824103/suppl/GSM824103_iCell_0.1_uM_6h_2.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824104 | GPL570 |
|
iCell_0.1 μM_6 h_rep3
|
iCell human cardiomyocytes exposed to 0.1 μM VX 6 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_6h_3
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM824104
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824104/suppl/GSM824104_iCell_0.1_uM_6h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824105 | GPL570 |
|
iCell_0.1 μM_6 h_rep4
|
iCell human cardiomyocytes exposed to 0.1 μM VX 6 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_6h_4
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM824105
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824105/suppl/GSM824105_iCell_0.1_uM_6h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824106 | GPL570 |
|
iCell_0.1 μM_24 h_rep1
|
iCell human cardiomyocytes exposed to 0.1 μM VX 24 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_24h_1
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 24 h after exposure
|
| Sample_geo_accession | GSM824106
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824106/suppl/GSM824106_iCell_0.1_uM_24h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824107 | GPL570 |
|
iCell_0.1 μM_24 h_rep2
|
iCell human cardiomyocytes exposed to 0.1 μM VX 24 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_24h_2
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 24 h after exposure
|
| Sample_geo_accession | GSM824107
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824107/suppl/GSM824107_iCell_0.1_uM_24h_2.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824108 | GPL570 |
|
iCell_0.1 μM_24 h_rep3
|
iCell human cardiomyocytes exposed to 0.1 μM VX 24 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_24h_3
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 24 h after exposure
|
| Sample_geo_accession | GSM824108
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824108/suppl/GSM824108_iCell_0.1_uM_24h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824109 | GPL570 |
|
iCell_0.1 μM_24 h_rep4
|
iCell human cardiomyocytes exposed to 0.1 μM VX 24 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_24h_4
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 24 h after exposure
|
| Sample_geo_accession | GSM824109
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824109/suppl/GSM824109_iCell_0.1_uM_24h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824110 | GPL570 |
|
iCell_0.1 μM_72 h_rep1
|
iCell human cardiomyocytes exposed to 0.1 μM VX 72 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_72h_1
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 72 h after exposure
|
| Sample_geo_accession | GSM824110
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824110/suppl/GSM824110_iCell_0.1_uM_72h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824111 | GPL570 |
|
iCell_0.1 μM_72 h_rep2
|
iCell human cardiomyocytes exposed to 0.1 μM VX 72 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_72h_2
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 72 h after exposure
|
| Sample_geo_accession | GSM824111
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824111/suppl/GSM824111_iCell_0.1_uM_72h_2.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824112 | GPL570 |
|
iCell_0.1 μM_72 h_rep3
|
iCell human cardiomyocytes exposed to 0.1 μM VX 72 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_72h_3
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 72 h after exposure
|
| Sample_geo_accession | GSM824112
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824112/suppl/GSM824112_iCell_0.1_uM_72h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824113 | GPL570 |
|
iCell_0.1 μM_72 h_rep4
|
iCell human cardiomyocytes exposed to 0.1 μM VX 72 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_0.1 uM_72h_4
gene expression data of iCell human cardiomyocytes exposed to 0.1 μM VX 72 h after exposure
|
| Sample_geo_accession | GSM824113
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824113/suppl/GSM824113_iCell_0.1_uM_72h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824114 | GPL570 |
|
iCell_10 μM_6 h_rep1
|
iCell human cardiomyocytes exposed to 10 μM VX 6 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_6h_1
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM824114
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824114/suppl/GSM824114_iCell_10_uM_6h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824115 | GPL570 |
|
iCell_10 μM_6 h_rep2
|
iCell human cardiomyocytes exposed to 10 μM VX 6 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_6h_2
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM824115
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824115/suppl/GSM824115_iCell_10_uM_6h_2.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824116 | GPL570 |
|
iCell_10 μM_6 h_rep3
|
iCell human cardiomyocytes exposed to 10 μM VX 6 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_6h_3
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM824116
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824116/suppl/GSM824116_iCell_10_uM_6h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824117 | GPL570 |
|
iCell_10 μM_6 h_rep4
|
iCell human cardiomyocytes exposed to 10 μM VX 6 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_6h_4
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM824117
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824117/suppl/GSM824117_iCell_10_uM_6h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824118 | GPL570 |
|
iCell_10 μM_24 h_rep1
|
iCell human cardiomyocytes exposed to 10 μM VX 24 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_24h_1
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 24 h after exposure
|
| Sample_geo_accession | GSM824118
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824118/suppl/GSM824118_iCell_10_uM_24h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824119 | GPL570 |
|
iCell_10 μM_24 h_rep3
|
iCell human cardiomyocytes exposed to 10 μM VX 24 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_24h_3
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 24 h after exposure
|
| Sample_geo_accession | GSM824119
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824119/suppl/GSM824119_iCell_10_uM_24h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824120 | GPL570 |
|
iCell_10 μM_24 h_rep4
|
iCell human cardiomyocytes exposed to 10 μM VX 24 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_24h_4
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 24 h after exposure
|
| Sample_geo_accession | GSM824120
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824120/suppl/GSM824120_iCell_10_uM_24h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824121 | GPL570 |
|
iCell_10 μM_72 h_rep1
|
iCell human cardiomyocytes exposed to 10 μM VX 72 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_72h_1
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 72 h after exposure
|
| Sample_geo_accession | GSM824121
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824121/suppl/GSM824121_iCell_10_uM_72h_1.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824122 | GPL570 |
|
iCell_10 μM_72 h_rep2
|
iCell human cardiomyocytes exposed to 10 μM VX 72 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_72h_2
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 72 h after exposure
|
| Sample_geo_accession | GSM824122
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824122/suppl/GSM824122_iCell_10_uM_72h_2.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824123 | GPL570 |
|
iCell_10 μM_72 h_rep3
|
iCell human cardiomyocytes exposed to 10 μM VX 72 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_72h_3
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 72 h after exposure
|
| Sample_geo_accession | GSM824123
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824123/suppl/GSM824123_iCell_10_uM_72h_3.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
| | |
|
GSM824124 | GPL570 |
|
iCell_10 μM_72 h_rep4
|
iCell human cardiomyocytes exposed to 10 μM VX 72 h after exposure
|
cell type: iCell cardiomyocytes derived from WA09 human embryonic stem cells
|
iCell_10 uM_72h_4
gene expression data of iCell human cardiomyocytes exposed to 10 μM VX 72 h after exposure
|
| Sample_geo_accession | GSM824124
| | Sample_status | Public on Oct 29 2011
| | Sample_submission_date | Oct 28 2011
| | Sample_last_update_date | Oct 29 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human iCell cardiomyocytes were seeded in 6-well plates in media formulated by the manufacturer (Cellular Dynamics International, Madison, WI) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM824nnn/GSM824124/suppl/GSM824124_iCell_10_uM_72h_4.CEL.gz
| | Sample_series_id | GSE33325
| | Sample_data_row_count | 54675
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