Search results for the GEO ID: GSE33455  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM827130 | GPL570 |
|
DU-145-rep1
|
DU-145 cell line original
|
cell line: DU-145
disease state: metastatic prostate cancer
treatment: none
|
|
| Sample_geo_accession | GSM827130
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827130/suppl/GSM827130.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827131 | GPL570 |
|
DU-145-rep2
|
DU-145 cell line original
|
cell line: DU-145
disease state: metastatic prostate cancer
treatment: none
|
|
| Sample_geo_accession | GSM827131
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827131/suppl/GSM827131.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827132 | GPL570 |
|
DU-145-rep3
|
DU-145 cell line original
|
cell line: DU-145
disease state: metastatic prostate cancer
treatment: none
|
|
| Sample_geo_accession | GSM827132
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827132/suppl/GSM827132.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827133 | GPL570 |
|
DU-145-docetaxel resistant-rep1
|
DU-145 cell line resistant to docetaxel
|
cell line: DU-145
disease state: metastatic prostate cancer
treatment: converted to docetaxel-resistant cells
|
|
| Sample_geo_accession | GSM827133
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827133/suppl/GSM827133.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827134 | GPL570 |
|
DU-145-docetaxel resistant-rep2
|
DU-145 cell line resistant to docetaxel
|
cell line: DU-145
disease state: metastatic prostate cancer
treatment: converted to docetaxel-resistant cells
|
|
| Sample_geo_accession | GSM827134
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827134/suppl/GSM827134.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827135 | GPL570 |
|
DU-145-docetaxel resistant-rep3
|
DU-145 cell line resistant to docetaxel
|
cell line: DU-145
disease state: metastatic prostate cancer
treatment: converted to docetaxel-resistant cells
|
|
| Sample_geo_accession | GSM827135
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827135/suppl/GSM827135.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827136 | GPL570 |
|
PC3-1
|
PC3 cell line original
|
cell line: PC3
disease state: metastatic prostate cancer
treatment: none
|
|
| Sample_geo_accession | GSM827136
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827136/suppl/GSM827136.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827137 | GPL570 |
|
PC3-2
|
PC3 cell line original
|
cell line: PC3
disease state: metastatic prostate cancer
treatment: none
|
|
| Sample_geo_accession | GSM827137
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827137/suppl/GSM827137.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827138 | GPL570 |
|
PC3-3
|
PC3 cell line original
|
cell line: PC3
disease state: metastatic prostate cancer
treatment: none
|
|
| Sample_geo_accession | GSM827138
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827138/suppl/GSM827138.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827139 | GPL570 |
|
PC3-docetaxel resistant-rep1
|
PC3 cell line resistant to docetaxel
|
cell line: PC3
disease state: metastatic prostate cancer
treatment: converted to docetaxel-resistant cells
|
|
| Sample_geo_accession | GSM827139
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827139/suppl/GSM827139.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
| | |
|
GSM827140 | GPL570 |
|
PC3-docetaxel resistant-rep2
|
PC3 cell line resistant to docetaxel
|
cell line: PC3
disease state: metastatic prostate cancer
treatment: converted to docetaxel-resistant cells
|
|
| Sample_geo_accession | GSM827140
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827140/suppl/GSM827140.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
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GSM827141 | GPL570 |
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PC3-docetaxel resistant-rep3
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PC3 cell line resistant to docetaxel
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cell line: PC3
disease state: metastatic prostate cancer
treatment: converted to docetaxel-resistant cells
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| Sample_geo_accession | GSM827141
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | Nov 03 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).
| | Sample_growth_protocol_ch1 | DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| | Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| | Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| | Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mercedes,,Marín-Aguilera
| | Sample_contact_email | mmarin1@clinic.ub.es
| | Sample_contact_institute | Fundació Clínic per a la Recerca Biomèdica
| | Sample_contact_address | Villarroel 170
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08036
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM827nnn/GSM827141/suppl/GSM827141.CEL.gz
| | Sample_series_id | GSE33455
| | Sample_data_row_count | 54675
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