Search results for the GEO ID: GSE33513  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM829333 | GPL1261 |
|
Tcf1-/- ETP thymocyte, biological rep1
|
mouse DN1 stage thymocytes, Lin-CD44+CD25-kit+.
|
tissue: thymus
genotype: Lin-CD44+CD25-kit+
age: DN1 thymocyte, 10 weeks
genotype/variation: Tcf1-/-
background strain: C57Bl/6
|
Gene expression data from mouse ETP stage thymocytes with ablation of Tcf1.
|
| Sample_geo_accession | GSM829333
| | Sample_status | Public on Dec 06 2011
| | Sample_submission_date | Nov 07 2011
| | Sample_last_update_date | Dec 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were sacrificed at 10 weeks of age
| | Sample_growth_protocol_ch1 | Tcf1-/- and Tcf1-/+ strains were bred and housed under pathogen-free conditions
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Arcturus PicoPure RNA Isolation kit (Life Technologies, Foster City, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Average yield from 1000 cells was 3 ng of total RNA. The RNA was amplified using Ovation Pico WTA System (NuGen, San Carlos, CA). Labeling, fragmentation and hybridization to Mouse Genome 430 2.0 Arrays were done according to the manufacturer's instructions (Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with affy bioconductor package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM829nnn/GSM829333/suppl/GSM829333.CEL.gz
| | Sample_series_id | GSE33513
| | Sample_data_row_count | 45101
| | |
|
GSM829334 | GPL1261 |
|
Tcf1-/- ETP thymocyte, biological rep2
|
mouse DN1 stage thymocytes, Lin-CD44+CD25-kit+.
|
tissue: thymus
genotype: Lin-CD44+CD25-kit+
age: DN1 thymocyte, 10 weeks
genotype/variation: Tcf1-/-
background strain: C57Bl/6
|
Gene expression data from mouse ETP stage thymocytes with ablation of Tcf1.
|
| Sample_geo_accession | GSM829334
| | Sample_status | Public on Dec 06 2011
| | Sample_submission_date | Nov 07 2011
| | Sample_last_update_date | Dec 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were sacrificed at 10 weeks of age
| | Sample_growth_protocol_ch1 | Tcf1-/- and Tcf1-/+ strains were bred and housed under pathogen-free conditions
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Arcturus PicoPure RNA Isolation kit (Life Technologies, Foster City, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Average yield from 1000 cells was 3 ng of total RNA. The RNA was amplified using Ovation Pico WTA System (NuGen, San Carlos, CA). Labeling, fragmentation and hybridization to Mouse Genome 430 2.0 Arrays were done according to the manufacturer's instructions (Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with affy bioconductor package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM829nnn/GSM829334/suppl/GSM829334.CEL.gz
| | Sample_series_id | GSE33513
| | Sample_data_row_count | 45101
| | |
|
GSM829335 | GPL1261 |
|
Tcf1-/- ETP thymocyte, biological rep3
|
mouse DN1 stage thymocytes, Lin-CD44+CD25-kit+.
|
tissue: thymus
genotype: Lin-CD44+CD25-kit+
age: DN1 thymocyte, 10 weeks
genotype/variation: Tcf1-/-
background strain: C57Bl/6
|
Gene expression data from mouse ETP stage thymocytes with ablation of Tcf1.
|
| Sample_geo_accession | GSM829335
| | Sample_status | Public on Dec 06 2011
| | Sample_submission_date | Nov 07 2011
| | Sample_last_update_date | Dec 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were sacrificed at 10 weeks of age
| | Sample_growth_protocol_ch1 | Tcf1-/- and Tcf1-/+ strains were bred and housed under pathogen-free conditions
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Arcturus PicoPure RNA Isolation kit (Life Technologies, Foster City, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Average yield from 1000 cells was 3 ng of total RNA. The RNA was amplified using Ovation Pico WTA System (NuGen, San Carlos, CA). Labeling, fragmentation and hybridization to Mouse Genome 430 2.0 Arrays were done according to the manufacturer's instructions (Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with affy bioconductor package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM829nnn/GSM829335/suppl/GSM829335.CEL.gz
| | Sample_series_id | GSE33513
| | Sample_data_row_count | 45101
| | |
|
GSM829336 | GPL1261 |
|
Tcf1-/+ ETP thymocyte, biological rep1
|
mouse DN1 stage thymocytes, Lin-CD44+CD25-kit+.
|
tissue: thymus
genotype: Lin-CD44+CD25-kit+
age: DN1 thymocyte, 10 weeks
genotype/variation: wild type
background strain: C57Bl/6
|
Gene expression data from mouse ETP stage Tcf1-/+ thymocytes.
|
| Sample_geo_accession | GSM829336
| | Sample_status | Public on Dec 06 2011
| | Sample_submission_date | Nov 07 2011
| | Sample_last_update_date | Dec 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were sacrificed at 10 weeks of age
| | Sample_growth_protocol_ch1 | Tcf1-/- and Tcf1-/+ strains were bred and housed under pathogen-free conditions
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Arcturus PicoPure RNA Isolation kit (Life Technologies, Foster City, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Average yield from 1000 cells was 3 ng of total RNA. The RNA was amplified using Ovation Pico WTA System (NuGen, San Carlos, CA). Labeling, fragmentation and hybridization to Mouse Genome 430 2.0 Arrays were done according to the manufacturer's instructions (Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with affy bioconductor package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM829nnn/GSM829336/suppl/GSM829336.CEL.gz
| | Sample_series_id | GSE33513
| | Sample_data_row_count | 45101
| | |
|
GSM829337 | GPL1261 |
|
Tcf1-/+ ETP thymocyte, biological rep2
|
mouse DN1 stage thymocytes, Lin-CD44+CD25-kit+.
|
tissue: thymus
genotype: Lin-CD44+CD25-kit+
age: DN1 thymocyte, 10 weeks
genotype/variation: wild type
background strain: C57Bl/6
|
Gene expression data from mouse ETP stage Tcf1-/+ thymocytes.
|
| Sample_geo_accession | GSM829337
| | Sample_status | Public on Dec 06 2011
| | Sample_submission_date | Nov 07 2011
| | Sample_last_update_date | Dec 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were sacrificed at 10 weeks of age
| | Sample_growth_protocol_ch1 | Tcf1-/- and Tcf1-/+ strains were bred and housed under pathogen-free conditions
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Arcturus PicoPure RNA Isolation kit (Life Technologies, Foster City, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Average yield from 1000 cells was 3 ng of total RNA. The RNA was amplified using Ovation Pico WTA System (NuGen, San Carlos, CA). Labeling, fragmentation and hybridization to Mouse Genome 430 2.0 Arrays were done according to the manufacturer's instructions (Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with affy bioconductor package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM829nnn/GSM829337/suppl/GSM829337.CEL.gz
| | Sample_series_id | GSE33513
| | Sample_data_row_count | 45101
| | |
|
GSM829338 | GPL1261 |
|
Tcf1-/+ ETP thymocyte, biological rep3
|
mouse DN1 stage thymocytes, Lin-CD44+CD25-kit+.
|
tissue: thymus
genotype: Lin-CD44+CD25-kit+
age: DN1 thymocyte, 10 weeks
genotype/variation: wild type
background strain: C57Bl/6
|
Gene expression data from mouse ETP stage Tcf1-/+ thymocytes.
|
| Sample_geo_accession | GSM829338
| | Sample_status | Public on Dec 06 2011
| | Sample_submission_date | Nov 07 2011
| | Sample_last_update_date | Dec 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were sacrificed at 10 weeks of age
| | Sample_growth_protocol_ch1 | Tcf1-/- and Tcf1-/+ strains were bred and housed under pathogen-free conditions
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Arcturus PicoPure RNA Isolation kit (Life Technologies, Foster City, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Average yield from 1000 cells was 3 ng of total RNA. The RNA was amplified using Ovation Pico WTA System (NuGen, San Carlos, CA). Labeling, fragmentation and hybridization to Mouse Genome 430 2.0 Arrays were done according to the manufacturer's instructions (Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with affy bioconductor package.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiangwen,,Zhang
| | Sample_contact_email | jzhang@cgr.harvard.edu
| | Sample_contact_department | FAS Center for Systems Biology
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 7 Divinity Ave
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02138
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM829nnn/GSM829338/suppl/GSM829338.CEL.gz
| | Sample_series_id | GSE33513
| | Sample_data_row_count | 45101
| | |
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