Search results for the GEO ID: GSE33606  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM830925 | GPL570 |
|
hNHEPS_control_0 h_rep1
|
hNHEPS human hepatocytes not exposed to VX at the beginning of experiment
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 0
dose: 0
agent: none
|
gene expression data of hNHEPS human hepatocytes not exposed to VX at the beginning of experiment
|
| Sample_geo_accession | GSM830925
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830925/suppl/GSM830925_hNHEPS_0_uM_0_h_1.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830926 | GPL570 |
|
hNHEPS_control_0 h_rep2
|
hNHEPS human hepatocytes not exposed to VX at the beginning of experiment
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 0
dose: 0
agent: none
|
gene expression data of hNHEPS human hepatocytes not exposed to VX at the beginning of experiment
|
| Sample_geo_accession | GSM830926
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830926/suppl/GSM830926_hNHEPS_0_uM_0_h_2.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830927 | GPL570 |
|
hNHEPS_control_0 h_rep3
|
hNHEPS human hepatocytes not exposed to VX at the beginning of experiment
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 0
dose: 0
agent: none
|
gene expression data of hNHEPS human hepatocytes not exposed to VX at the beginning of experiment
|
| Sample_geo_accession | GSM830927
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830927/suppl/GSM830927_hNHEPS_0_uM_0_h_3.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830928 | GPL570 |
|
hNHEPS_control_0 h_rep4
|
hNHEPS human hepatocytes not exposed to VX at the beginning of experiment
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 0
dose: 0
agent: none
|
gene expression data of hNHEPS human hepatocytes not exposed to VX at the beginning of experiment
|
| Sample_geo_accession | GSM830928
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830928/suppl/GSM830928_hNHEPS_0_uM_0_h_4.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830929 | GPL570 |
|
hNHEPS_control_6 h_rep1
|
hNHEPS human hepatocytes not exposed to VX at 6 h
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 0
agent: none
|
gene expression data of hNHEPS human hepatocytes not exposed to VX at 6 h
|
| Sample_geo_accession | GSM830929
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830929/suppl/GSM830929_hNHEPS_0_uM_6_h_1.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830930 | GPL570 |
|
hNHEPS_control_6 h_rep2
|
hNHEPS human hepatocytes not exposed to VX at 6 h
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 0
agent: none
|
gene expression data of hNHEPS human hepatocytes not exposed to VX at 6 h
|
| Sample_geo_accession | GSM830930
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830930/suppl/GSM830930_hNHEPS_0_uM_6_h_2.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830931 | GPL570 |
|
hNHEPS_control_6 h_rep4
|
hNHEPS human hepatocytes not exposed to VX at 6 h
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 0
agent: none
|
gene expression data of hNHEPS human hepatocytes not exposed to VX at 6 h
|
| Sample_geo_accession | GSM830931
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830931/suppl/GSM830931_hNHEPS_0_uM_6_h_4.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830932 | GPL570 |
|
hNHEPS_0.1 μM_6 h_rep1
|
hNHEPS human hepatocytes exposed to 0.1 μM VX 6 h after exposure
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 0.1 μM
agent: VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothiolate)
|
gene expression data of hNHEPS human hepatocytes exposed to 0.1 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM830932
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830932/suppl/GSM830932_hNHEPS_0.1_uM_6_h_1.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830933 | GPL570 |
|
hNHEPS_0.1 μM_6 h_rep2
|
hNHEPS human hepatocytes exposed to 0.1 μM VX 6 h after exposure
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 0.1 μM
agent: VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothiolate)
|
gene expression data of hNHEPS human hepatocytes exposed to 0.1 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM830933
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830933/suppl/GSM830933_hNHEPS_0.1_uM_6_h_2.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830934 | GPL570 |
|
hNHEPS_0.1 μM_6 h_rep3
|
hNHEPS human hepatocytes exposed to 0.1 μM VX 6 h after exposure
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 0.1 μM
agent: VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothiolate)
|
gene expression data of hNHEPS human hepatocytes exposed to 0.1 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM830934
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830934/suppl/GSM830934_hNHEPS_0.1_uM_6_h_3.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830935 | GPL570 |
|
hNHEPS_0.1 μM_6 h_rep4
|
hNHEPS human hepatocytes exposed to 0.1 μM VX 6 h after exposure
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 0.1 μM
agent: VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothiolate)
|
gene expression data of hNHEPS human hepatocytes exposed to 0.1 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM830935
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830935/suppl/GSM830935_hNHEPS_0.1_uM_6_h_4.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830936 | GPL570 |
|
hNHEPS_10 μM_6 h_rep1
|
hNHEPS human hepatocytes exposed to 10 μM VX 6 h after exposure
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 10 μM
agent: VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothiolate)
|
gene expression data of hNHEPS human hepatocytes exposed to 10 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM830936
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830936/suppl/GSM830936_hNHEPS_10_uM_6_h_1.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830937 | GPL570 |
|
hNHEPS_10 μM_6 h_rep2
|
hNHEPS human hepatocytes exposed to 10 μM VX 6 h after exposure
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 10 μM
agent: VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothiolate)
|
gene expression data of hNHEPS human hepatocytes exposed to 10 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM830937
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830937/suppl/GSM830937_hNHEPS_10_uM_6_h_2.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830938 | GPL570 |
|
hNHEPS_10 μM_6 h_rep3
|
hNHEPS human hepatocytes exposed to 10 μM VX 6 h after exposure
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 10 μM
agent: VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothiolate)
|
gene expression data of hNHEPS human hepatocytes exposed to 10 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM830938
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830938/suppl/GSM830938_hNHEPS_10_uM_6_h_3.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
GSM830939 | GPL570 |
|
hNHEPS_10 μM_6 h_rep4
|
hNHEPS human hepatocytes exposed to 10 μM VX 6 h after exposure
|
cell type: Normal human primary hepatocytes isolated from non-transplantable donor tissue
time point: 6 h
dose: 10 μM
agent: VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothiolate)
|
gene expression data of hNHEPS human hepatocytes exposed to 10 μM VX 6 h after exposure
|
| Sample_geo_accession | GSM830939
| | Sample_status | Public on Nov 11 2011
| | Sample_submission_date | Nov 10 2011
| | Sample_last_update_date | Nov 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | VX was added to cell culture at the specified concentrations and maintained for 1 h. Then VX was removed and the culture was washed 3X with fresh medium. The culture was then maintained at 37 °C with 5% CO2 until the indicated time points.
| | Sample_growth_protocol_ch1 | Human hNHEPS hepatocytes were seeded in 6-well plates in media formulated by the manufacturer (Lonza, Walkersville, MD) and cultured at 37 °C under humidified 5% CO2 for 48 h before VX exposure.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Plus Mini Kit from Qiagen (Valencia, CA) following the manufacturer’s protocol, including an on-column DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | ~0.1 μg of total RNA were labeled using the Affymetrix (Santa Clara, CA) GeneChip 3’ IVT Express Kit following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridization was carried out using the Affymetrix GeneChip Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
| | Sample_scan_protocol | The chips were scanned using an Affymetrix GeneChip Instrument System and the image (.DAT) files were processed using the Affymetrix GeneChip Operating Software (GCOS) to generate cell intensity (.CEL) files.
| | Sample_data_processing | The data were averaged and normalized in Partek Genomics Suite 6.5 (Partek, St. Louis, MO) using the robust multiarray analysis (RMA) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Xiugong,,Gao
| | Sample_contact_email | xiugong.gao@amedd.army.mil
| | Sample_contact_institute | Walter Reed Army Institute of Research
| | Sample_contact_address | 503 Robert Grant Ave
| | Sample_contact_city | Silver Spring
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20910
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM830nnn/GSM830939/suppl/GSM830939_hNHEPS_10_uM_6_h_4.CEL.gz
| | Sample_series_id | GSE33606
| | Sample_data_row_count | 54675
| | |
|
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