Result

Search results for the GEO ID: GSE33659

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM832412

GPL1261

CBFb_KD_1

CBFb_KD

cell line: L8057 cell type: megakaryocytic cells genotype/variation: shRNA-mediated CBFb knockdown

maturation was induced by adding 50 nM (final concentration) 12-O- tetradecanoylphorbol-13-acetate (TPA) to the medium for 3 days

Sample_geo_accession	GSM832412
Sample_status	Public on Feb 10 2012
Sample_submission_date	Nov 14 2011
Sample_last_update_date	Feb 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	48 hrs after lenti-viral infection, 2 microgram/microliter puromycin was added to culture medium for selecting infected cells, and cells were harvested for RNA 72 hrs after puromycin selection. 50 nM TPA was added at the same time with puromycin to induced maturation of the cells.
Sample_growth_protocol_ch1	Cells were grown in 38.5% IMDM + 38.5% RPMI + 20% FCS + %2 Pen/strep + 1% L-Glutamine,
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, catalogue no. 74134).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 microgram RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates.
Sample_hyb_protocol	hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
Sample_scan_protocol	Scanned with GeneChIP scanner 300 7G using the standard protocol.
Sample_data_processing	Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/).
Sample_platform_id	GPL1261
Sample_contact_name	Tali,,Mazor
Sample_contact_institute	MIT
Sample_contact_address	77 Massachusetts Ave
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02139
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832412/suppl/GSM832412_CBFb_1.CEL.gz
Sample_series_id	GSE33659
Sample_series_id	GSE33660
Sample_data_row_count	45101

GSM832413

GPL1261

CBFb_KD_2

CBFb_KD

cell line: L8057 cell type: megakaryocytic cells genotype/variation: shRNA-mediated CBFb knockdown

maturation was induced by adding 50 nM (final concentration) 12-O- tetradecanoylphorbol-13-acetate (TPA) to the medium for 3 days

Sample_geo_accession	GSM832413
Sample_status	Public on Feb 10 2012
Sample_submission_date	Nov 14 2011
Sample_last_update_date	Feb 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	48 hrs after lenti-viral infection, 2 microgram/microliter puromycin was added to culture medium for selecting infected cells, and cells were harvested for RNA 72 hrs after puromycin selection. 50 nM TPA was added at the same time with puromycin to induced maturation of the cells.
Sample_growth_protocol_ch1	Cells were grown in 38.5% IMDM + 38.5% RPMI + 20% FCS + %2 Pen/strep + 1% L-Glutamine,
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, catalogue no. 74134).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 microgram RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates.
Sample_hyb_protocol	hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
Sample_scan_protocol	Scanned with GeneChIP scanner 300 7G using the standard protocol.
Sample_data_processing	Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/).
Sample_platform_id	GPL1261
Sample_contact_name	Tali,,Mazor
Sample_contact_institute	MIT
Sample_contact_address	77 Massachusetts Ave
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02139
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832413/suppl/GSM832413_CBFb_2.CEL.gz
Sample_series_id	GSE33659
Sample_series_id	GSE33660
Sample_data_row_count	45101

GSM832414

GPL1261

CBFb_KD_3

CBFb_KD

cell line: L8057 cell type: megakaryocytic cells genotype/variation: shRNA-mediated CBFb knockdown

maturation was induced by adding 50 nM (final concentration) 12-O- tetradecanoylphorbol-13-acetate (TPA) to the medium for 3 days

Sample_geo_accession	GSM832414
Sample_status	Public on Feb 10 2012
Sample_submission_date	Nov 14 2011
Sample_last_update_date	Feb 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	48 hrs after lenti-viral infection, 2 microgram/microliter puromycin was added to culture medium for selecting infected cells, and cells were harvested for RNA 72 hrs after puromycin selection. 50 nM TPA was added at the same time with puromycin to induced maturation of the cells.
Sample_growth_protocol_ch1	Cells were grown in 38.5% IMDM + 38.5% RPMI + 20% FCS + %2 Pen/strep + 1% L-Glutamine,
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, catalogue no. 74134).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 microgram RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates.
Sample_hyb_protocol	hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
Sample_scan_protocol	Scanned with GeneChIP scanner 300 7G using the standard protocol.
Sample_data_processing	Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/).
Sample_platform_id	GPL1261
Sample_contact_name	Tali,,Mazor
Sample_contact_institute	MIT
Sample_contact_address	77 Massachusetts Ave
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02139
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832414/suppl/GSM832414_CBFb_3.CEL.gz
Sample_series_id	GSE33659
Sample_series_id	GSE33660
Sample_data_row_count	45101

GSM832415

GPL1261

Ring1b_KD_1

Ring1b_KD

cell line: L8057 cell type: megakaryocytic cells genotype/variation: shRNA-mediated Ring1b knockdown

maturation was induced by adding 50 nM (final concentration) 12-O- tetradecanoylphorbol-13-acetate (TPA) to the medium for 3 days

Sample_geo_accession	GSM832415
Sample_status	Public on Feb 10 2012
Sample_submission_date	Nov 14 2011
Sample_last_update_date	Feb 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	48 hrs after lenti-viral infection, 2 microgram/microliter puromycin was added to culture medium for selecting infected cells, and cells were harvested for RNA 72 hrs after puromycin selection. 50 nM TPA was added at the same time with puromycin to induced maturation of the cells.
Sample_growth_protocol_ch1	Cells were grown in 38.5% IMDM + 38.5% RPMI + 20% FCS + %2 Pen/strep + 1% L-Glutamine,
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, catalogue no. 74134).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 microgram RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates.
Sample_hyb_protocol	hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
Sample_scan_protocol	Scanned with GeneChIP scanner 300 7G using the standard protocol.
Sample_data_processing	Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/).
Sample_platform_id	GPL1261
Sample_contact_name	Tali,,Mazor
Sample_contact_institute	MIT
Sample_contact_address	77 Massachusetts Ave
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02139
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832415/suppl/GSM832415_Ring1b_1.CEL.gz
Sample_series_id	GSE33659
Sample_series_id	GSE33660
Sample_data_row_count	45101

GSM832416

GPL1261

Ring1b_KD_2

Ring1b_KD

cell line: L8057 cell type: megakaryocytic cells genotype/variation: shRNA-mediated Ring1b knockdown

maturation was induced by adding 50 nM (final concentration) 12-O- tetradecanoylphorbol-13-acetate (TPA) to the medium for 3 days

Sample_geo_accession	GSM832416
Sample_status	Public on Feb 10 2012
Sample_submission_date	Nov 14 2011
Sample_last_update_date	Feb 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	48 hrs after lenti-viral infection, 2 microgram/microliter puromycin was added to culture medium for selecting infected cells, and cells were harvested for RNA 72 hrs after puromycin selection. 50 nM TPA was added at the same time with puromycin to induced maturation of the cells.
Sample_growth_protocol_ch1	Cells were grown in 38.5% IMDM + 38.5% RPMI + 20% FCS + %2 Pen/strep + 1% L-Glutamine,
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, catalogue no. 74134).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 microgram RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates.
Sample_hyb_protocol	hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
Sample_scan_protocol	Scanned with GeneChIP scanner 300 7G using the standard protocol.
Sample_data_processing	Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/).
Sample_platform_id	GPL1261
Sample_contact_name	Tali,,Mazor
Sample_contact_institute	MIT
Sample_contact_address	77 Massachusetts Ave
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02139
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832416/suppl/GSM832416_Ring1b_2.CEL.gz
Sample_series_id	GSE33659
Sample_series_id	GSE33660
Sample_data_row_count	45101

GSM832417

GPL1261

Ring1b_KD_3

Ring1b_KD

cell line: L8057 cell type: megakaryocytic cells genotype/variation: shRNA-mediated Ring1b knockdown

maturation was induced by adding 50 nM (final concentration) 12-O- tetradecanoylphorbol-13-acetate (TPA) to the medium for 3 days

Sample_geo_accession	GSM832417
Sample_status	Public on Feb 10 2012
Sample_submission_date	Nov 14 2011
Sample_last_update_date	Feb 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	48 hrs after lenti-viral infection, 2 microgram/microliter puromycin was added to culture medium for selecting infected cells, and cells were harvested for RNA 72 hrs after puromycin selection. 50 nM TPA was added at the same time with puromycin to induced maturation of the cells.
Sample_growth_protocol_ch1	Cells were grown in 38.5% IMDM + 38.5% RPMI + 20% FCS + %2 Pen/strep + 1% L-Glutamine,
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, catalogue no. 74134).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 microgram RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates.
Sample_hyb_protocol	hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
Sample_scan_protocol	Scanned with GeneChIP scanner 300 7G using the standard protocol.
Sample_data_processing	Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/).
Sample_platform_id	GPL1261
Sample_contact_name	Tali,,Mazor
Sample_contact_institute	MIT
Sample_contact_address	77 Massachusetts Ave
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02139
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832417/suppl/GSM832417_Ring1b_3.CEL.gz
Sample_series_id	GSE33659
Sample_series_id	GSE33660
Sample_data_row_count	45101

GSM832418

GPL1261

Control_1

Control

cell line: L8057 cell type: megakaryocytic cells genotype/variation: empty vector control

maturation was induced by adding 50 nM (final concentration) 12-O- tetradecanoylphorbol-13-acetate (TPA) to the medium for 3 days

Sample_geo_accession	GSM832418
Sample_status	Public on Feb 10 2012
Sample_submission_date	Nov 14 2011
Sample_last_update_date	Feb 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	48 hrs after lenti-viral infection, 2 microgram/microliter puromycin was added to culture medium for selecting infected cells, and cells were harvested for RNA 72 hrs after puromycin selection. 50 nM TPA was added at the same time with puromycin to induced maturation of the cells.
Sample_growth_protocol_ch1	Cells were grown in 38.5% IMDM + 38.5% RPMI + 20% FCS + %2 Pen/strep + 1% L-Glutamine,
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, catalogue no. 74134).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 microgram RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates.
Sample_hyb_protocol	hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
Sample_scan_protocol	Scanned with GeneChIP scanner 300 7G using the standard protocol.
Sample_data_processing	Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/).
Sample_platform_id	GPL1261
Sample_contact_name	Tali,,Mazor
Sample_contact_institute	MIT
Sample_contact_address	77 Massachusetts Ave
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02139
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832418/suppl/GSM832418_Vector_1.CEL.gz
Sample_series_id	GSE33659
Sample_series_id	GSE33660
Sample_data_row_count	45101

GSM832419

GPL1261

Control_2

Control

cell line: L8057 cell type: megakaryocytic cells genotype/variation: empty vector control

maturation was induced by adding 50 nM (final concentration) 12-O- tetradecanoylphorbol-13-acetate (TPA) to the medium for 3 days

Sample_geo_accession	GSM832419
Sample_status	Public on Feb 10 2012
Sample_submission_date	Nov 14 2011
Sample_last_update_date	Feb 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	48 hrs after lenti-viral infection, 2 microgram/microliter puromycin was added to culture medium for selecting infected cells, and cells were harvested for RNA 72 hrs after puromycin selection. 50 nM TPA was added at the same time with puromycin to induced maturation of the cells.
Sample_growth_protocol_ch1	Cells were grown in 38.5% IMDM + 38.5% RPMI + 20% FCS + %2 Pen/strep + 1% L-Glutamine,
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, catalogue no. 74134).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 microgram RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates.
Sample_hyb_protocol	hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
Sample_scan_protocol	Scanned with GeneChIP scanner 300 7G using the standard protocol.
Sample_data_processing	Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/).
Sample_platform_id	GPL1261
Sample_contact_name	Tali,,Mazor
Sample_contact_institute	MIT
Sample_contact_address	77 Massachusetts Ave
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02139
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832419/suppl/GSM832419_Vector_2.CEL.gz
Sample_series_id	GSE33659
Sample_series_id	GSE33660
Sample_data_row_count	45101

GSM832420

GPL1261

Control_3

Control

cell line: L8057 cell type: megakaryocytic cells genotype/variation: empty vector control

maturation was induced by adding 50 nM (final concentration) 12-O- tetradecanoylphorbol-13-acetate (TPA) to the medium for 3 days

Sample_geo_accession	GSM832420
Sample_status	Public on Feb 10 2012
Sample_submission_date	Nov 14 2011
Sample_last_update_date	Feb 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	48 hrs after lenti-viral infection, 2 microgram/microliter puromycin was added to culture medium for selecting infected cells, and cells were harvested for RNA 72 hrs after puromycin selection. 50 nM TPA was added at the same time with puromycin to induced maturation of the cells.
Sample_growth_protocol_ch1	Cells were grown in 38.5% IMDM + 38.5% RPMI + 20% FCS + %2 Pen/strep + 1% L-Glutamine,
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, catalogue no. 74134).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 microgram RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates.
Sample_hyb_protocol	hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
Sample_scan_protocol	Scanned with GeneChIP scanner 300 7G using the standard protocol.
Sample_data_processing	Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/).
Sample_platform_id	GPL1261
Sample_contact_name	Tali,,Mazor
Sample_contact_institute	MIT
Sample_contact_address	77 Massachusetts Ave
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02139
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832420/suppl/GSM832420_Vector_3.CEL.gz
Sample_series_id	GSE33659
Sample_series_id	GSE33660
Sample_data_row_count	45101

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