Search results for the GEO ID: GSE33688  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM832886 | GPL1261 |
|
S51
|
S51
|
genotype/varation: S5
cell type: mouse embryonic fibroblasts
stimulation: none
strain: C57BL/6
|
STAT5A-containing vector was introduced into Stat5-null MEFs by retroviral infection
|
| Sample_geo_accession | GSM832886
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832886/suppl/GSM832886_03-16-11_13Hennighausen_JY_Mouse430_2_13S5-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832886/suppl/GSM832886_03-16-11_13Hennighausen_JY_Mouse430_2_13S5-1.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832887 | GPL1261 |
|
S52
|
S52
|
genotype/varation: S5
cell type: mouse embryonic fibroblasts
stimulation: none
strain: C57BL/6
|
STAT5A-containing vector was introduced into Stat5-null MEFs by retroviral infection
|
| Sample_geo_accession | GSM832887
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832887/suppl/GSM832887_03-16-11_14Hennighausen_JY_Mouse430_2_14S5-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832887/suppl/GSM832887_03-16-11_14Hennighausen_JY_Mouse430_2_14S5-2.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832888 | GPL1261 |
|
S5-gh1
|
S5-gh1
|
genotype/varation: S5
cell type: mouse embryonic fibroblasts
stimulation: growth hormone for 120 minutes
strain: C57BL/6
|
STAT5A-containing vector was introduced into Stat5-null MEFs by retroviral infection
|
| Sample_geo_accession | GSM832888
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832888/suppl/GSM832888_03-16-11_15Hennighausen_JY_Mouse430_2_15S5-gh1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832888/suppl/GSM832888_03-16-11_15Hennighausen_JY_Mouse430_2_15S5-gh1.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832889 | GPL1261 |
|
S5-gh2
|
S5-gh2
|
genotype/varation: S5
cell type: mouse embryonic fibroblasts
stimulation: growth hormone for 120 minutes
strain: C57BL/6
|
STAT5A-containing vector was introduced into Stat5-null MEFs by retroviral infection
|
| Sample_geo_accession | GSM832889
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832889/suppl/GSM832889_03-16-11_16Hennighausen_JY_Mouse430_2_16S5-gh2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832889/suppl/GSM832889_03-16-11_16Hennighausen_JY_Mouse430_2_16S5-gh2.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832890 | GPL1261 |
|
WT1
|
WT1
|
genotype/varation: WT
cell type: mouse embryonic fibroblasts
stimulation: none
strain: C57BL/6
|
wild-type MEFs
|
| Sample_geo_accession | GSM832890
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832890/suppl/GSM832890_03-16-11_17Hennighausen_JY_Mouse430_2_17WT1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832890/suppl/GSM832890_03-16-11_17Hennighausen_JY_Mouse430_2_17WT1.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832891 | GPL1261 |
|
WT2
|
WT2
|
genotype/varation: WT
cell type: mouse embryonic fibroblasts
stimulation: none
strain: C57BL/6
|
wild-type MEFs
|
| Sample_geo_accession | GSM832891
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832891/suppl/GSM832891_03-16-11_18Hennighausen_JY_Mouse430_2_18WT2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832891/suppl/GSM832891_03-16-11_18Hennighausen_JY_Mouse430_2_18WT2.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832892 | GPL1261 |
|
WT-gh1
|
WT-gh1
|
genotype/varation: WT
cell type: mouse embryonic fibroblasts
stimulation: growth hormone for 120 minutes
strain: C57BL/6
|
wild-type MEFs
|
| Sample_geo_accession | GSM832892
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832892/suppl/GSM832892_03-16-11_19Hennighausen_JY_Mouse430_2_19WT-gh1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832892/suppl/GSM832892_03-16-11_19Hennighausen_JY_Mouse430_2_19WT-gh1.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832893 | GPL1261 |
|
WT-gh2
|
WT-gh2
|
genotype/varation: WT
cell type: mouse embryonic fibroblasts
stimulation: growth hormone for 120 minutes
strain: C57BL/6
|
wild-type MEFs
|
| Sample_geo_accession | GSM832893
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832893/suppl/GSM832893_03-16-11_20Hennighausen_JY_Mouse430_2_20WT-gh2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832893/suppl/GSM832893_03-16-11_20Hennighausen_JY_Mouse430_2_20WT-gh2.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832894 | GPL1261 |
|
KO2
|
KO2
|
genotype/varation: KO
cell type: mouse embryonic fibroblasts
stimulation: none
strain: C57BL/6
|
Stat5-null (knockout) MEFs
|
| Sample_geo_accession | GSM832894
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832894/suppl/GSM832894_03-16-11_2Hennighausen_JY_Mouse430_2_KO-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832894/suppl/GSM832894_03-16-11_2Hennighausen_JY_Mouse430_2_KO-2.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832895 | GPL1261 |
|
KO-gh1
|
KO-gh1
|
genotype/varation: KO
cell type: mouse embryonic fibroblasts
stimulation: growth hormone for 120 minutes
strain: C57BL/6
|
Stat5-null (knockout) MEFs
|
| Sample_geo_accession | GSM832895
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832895/suppl/GSM832895_03-16-11_3Hennighausen_JY_Mouse430_2_3KO-gh1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832895/suppl/GSM832895_03-16-11_3Hennighausen_JY_Mouse430_2_3KO-gh1.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832896 | GPL1261 |
|
KO-gh2
|
KO-gh2
|
genotype/varation: KO
cell type: mouse embryonic fibroblasts
stimulation: growth hormone for 120 minutes
strain: C57BL/6
|
Stat5-null (knockout) MEFs
|
| Sample_geo_accession | GSM832896
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832896/suppl/GSM832896_03-16-11_4Hennighausen_JY_Mouse430_2_4KO-gh2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832896/suppl/GSM832896_03-16-11_4Hennighausen_JY_Mouse430_2_4KO-gh2.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
|
GSM832902 | GPL1261 |
|
KO1
|
KO1
|
genotype/varation: KO
cell type: mouse embryonic fibroblasts
stimulation: none
strain: C57BL/6
|
Stat5-null (knockout) MEFs
|
| Sample_geo_accession | GSM832902
| | Sample_status | Public on Mar 14 2012
| | Sample_submission_date | Nov 14 2011
| | Sample_last_update_date | Mar 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA from each group of the MEFs was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Expression values were determined with GeneChip Operating Software (GCOS) v1.1.1 software. RMA signals were summarized using GeneSpring GX 10.0.1 (Agilent) and normalized by quantile normalization. All data analysis was performed with GeneSpring software GX 10.01.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832902/suppl/GSM832902_03-16-11_Hennighausen_JY_Mouse430_2_KO-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM832nnn/GSM832902/suppl/GSM832902_03-16-11_Hennighausen_JY_Mouse430_2_KO-1.CHP.gz
| | Sample_series_id | GSE33688
| | Sample_data_row_count | 45101
| | |
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