Search results for the GEO ID: GSE33825  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM837383 | GPL570 |
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AEPC-KIA rep1
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AEPC-1, treated with KIA
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tissue: human distal lung
age: 69 years old
gender: female
number: H-2
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| Sample_geo_accession | GSM837383
| | Sample_status | Public on Oct 11 2012
| | Sample_submission_date | Nov 19 2011
| | Sample_last_update_date | Oct 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | To induce ATII-differentiation, AEPCs were cultured on the mixture of Matrigel and collagen type I gel with medium consiting of DMEM+5%FCS+antiboitics+KGF+cAMP+IBMX.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA) and cleaned up using miRNeasy mini kit (Qiagen, Tokyo, Japan).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 100 ng total RNA according to the manufacturer's protocol using GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | The purified and fragmented cRNA (12.5 μg) was hybridized to GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix). The array was incubated for 16 hours at 45°C, then automatically washed and stained with GeneChip Hybridization, Wash and Stain Kit (Affymetrix).
| | Sample_scan_protocol | The probe array was scanned using a GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix Expression Console (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hiroshi,,Kubo
| | Sample_contact_email | hkubo@med.tohoku.ac.jp
| | Sample_contact_phone | +81-22-717-7184
| | Sample_contact_fax | +81-22-717-7576
| | Sample_contact_department | Department of Advanced Preventive Medicine for Infectious Disease, and Regenerative Medicine
| | Sample_contact_institute | Tohoku University Graduate School of Medicine
| | Sample_contact_address | 2-1 Seiryomachi, Aobaku
| | Sample_contact_city | Sendai
| | Sample_contact_state | Miyagi
| | Sample_contact_zip/postal_code | 980-8575
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.apmid.med.tohoku.ac.jp/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837383/suppl/GSM837383_AEPC_KIA_1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837383/suppl/GSM837383_AEPC_KIA_1.CHP.gz
| | Sample_series_id | GSE33825
| | Sample_data_row_count | 54675
| | |
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GSM837384 | GPL570 |
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AEPC-KIA rep2
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AEPC-2, treated with KIA
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tissue: human distal lung
age: 78 years old
gender: male
number: H-13A
|
|
| Sample_geo_accession | GSM837384
| | Sample_status | Public on Oct 11 2012
| | Sample_submission_date | Nov 19 2011
| | Sample_last_update_date | Oct 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | To induce ATII-differentiation, AEPCs were cultured on the mixture of Matrigel and collagen type I gel with medium consiting of DMEM+5%FCS+antiboitics+KGF+cAMP+IBMX.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA) and cleaned up using miRNeasy mini kit (Qiagen, Tokyo, Japan).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 100 ng total RNA according to the manufacturer's protocol using GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | The purified and fragmented cRNA (12.5 μg) was hybridized to GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix). The array was incubated for 16 hours at 45°C, then automatically washed and stained with GeneChip Hybridization, Wash and Stain Kit (Affymetrix).
| | Sample_scan_protocol | The probe array was scanned using a GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix Expression Console (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hiroshi,,Kubo
| | Sample_contact_email | hkubo@med.tohoku.ac.jp
| | Sample_contact_phone | +81-22-717-7184
| | Sample_contact_fax | +81-22-717-7576
| | Sample_contact_department | Department of Advanced Preventive Medicine for Infectious Disease, and Regenerative Medicine
| | Sample_contact_institute | Tohoku University Graduate School of Medicine
| | Sample_contact_address | 2-1 Seiryomachi, Aobaku
| | Sample_contact_city | Sendai
| | Sample_contact_state | Miyagi
| | Sample_contact_zip/postal_code | 980-8575
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.apmid.med.tohoku.ac.jp/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837384/suppl/GSM837384_AEPC_KIA_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837384/suppl/GSM837384_AEPC_KIA_2.CHP.gz
| | Sample_series_id | GSE33825
| | Sample_data_row_count | 54675
| | |
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GSM837385 | GPL570 |
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AEPC-KIA rep3
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AEPC-3, treated with KIA
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tissue: human distal lung
age: 59 years old
gender: male
number: H-14
|
|
| Sample_geo_accession | GSM837385
| | Sample_status | Public on Oct 11 2012
| | Sample_submission_date | Nov 19 2011
| | Sample_last_update_date | Oct 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | To induce ATII-differentiation, AEPCs were cultured on the mixture of Matrigel and collagen type I gel with medium consiting of DMEM+5%FCS+antiboitics+KGF+cAMP+IBMX.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA) and cleaned up using miRNeasy mini kit (Qiagen, Tokyo, Japan).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 100 ng total RNA according to the manufacturer's protocol using GeneChip 3' IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | The purified and fragmented cRNA (12.5 μg) was hybridized to GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix). The array was incubated for 16 hours at 45°C, then automatically washed and stained with GeneChip Hybridization, Wash and Stain Kit (Affymetrix).
| | Sample_scan_protocol | The probe array was scanned using a GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix Expression Console (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hiroshi,,Kubo
| | Sample_contact_email | hkubo@med.tohoku.ac.jp
| | Sample_contact_phone | +81-22-717-7184
| | Sample_contact_fax | +81-22-717-7576
| | Sample_contact_department | Department of Advanced Preventive Medicine for Infectious Disease, and Regenerative Medicine
| | Sample_contact_institute | Tohoku University Graduate School of Medicine
| | Sample_contact_address | 2-1 Seiryomachi, Aobaku
| | Sample_contact_city | Sendai
| | Sample_contact_state | Miyagi
| | Sample_contact_zip/postal_code | 980-8575
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.apmid.med.tohoku.ac.jp/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837385/suppl/GSM837385_AEPC_KIA_3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837385/suppl/GSM837385_AEPC_KIA_3.CHP.gz
| | Sample_series_id | GSE33825
| | Sample_data_row_count | 54675
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