Search results for the GEO ID: GSE33845  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM837728 | GPL570 |
|
CL1-0_no EF stimulation_biological rep1
|
lung cancer CL1-0 cell line, no EF stimulation
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-0 cells without dcEF stimulation.
|
| Sample_geo_accession | GSM837728
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837728/suppl/GSM837728.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837728/suppl/GSM837728.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837729 | GPL570 |
|
CL1-0_no EF stimulation_biological rep2
|
lung cancer CL1-0 cell line, no EF stimulation
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-0 cells without dcEF stimulation.
|
| Sample_geo_accession | GSM837729
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837729/suppl/GSM837729.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837729/suppl/GSM837729.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837730 | GPL570 |
|
CL1-0_no EF stimulation_biological rep3
|
lung cancer CL1-0 cell line, no EF stimulation
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-0 cells without dcEF stimulation.
|
| Sample_geo_accession | GSM837730
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837730/suppl/GSM837730.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837730/suppl/GSM837730.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837731 | GPL570 |
|
CL1-5_no EF stimulation_biological rep1
|
lung cancer CL1-5 cell line, no EF stimulation
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-5 cells without dcEF stimulation.
|
| Sample_geo_accession | GSM837731
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837731/suppl/GSM837731.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837731/suppl/GSM837731.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837732 | GPL570 |
|
CL1-5_no EF stimulation_biological rep2
|
lung cancer CL1-5 cell line, no EF stimulation
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-5 cells without dcEF stimulation.
|
| Sample_geo_accession | GSM837732
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837732/suppl/GSM837732.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837732/suppl/GSM837732.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837733 | GPL570 |
|
CL1-5_no EF stimulation_biological rep3
|
lung cancer CL1-5 cell line, no EF stimulation
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-5 cells without dcEF stimulation.
|
| Sample_geo_accession | GSM837733
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837733/suppl/GSM837733.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837733/suppl/GSM837733.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837734 | GPL570 |
|
CL1-0_EF stimulation_biological rep1
|
lung cancer CL1-0 cell line, EF stimulation, 300mV/mm, 2hr
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-0 cells with dcEF stimulation.
|
| Sample_geo_accession | GSM837734
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837734/suppl/GSM837734.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837734/suppl/GSM837734.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837735 | GPL570 |
|
CL1-0_EF stimulation_biological rep2
|
lung cancer CL1-0 cell line, EF stimulation, 300mV/mm, 2hr
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-0 cells with dcEF stimulation.
|
| Sample_geo_accession | GSM837735
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837735/suppl/GSM837735.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837735/suppl/GSM837735.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837736 | GPL570 |
|
CL1-0_EF stimulation_biological rep3
|
lung cancer CL1-0 cell line, EF stimulation, 300mV/mm, 2hr
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-0 cells with dcEF stimulation.
|
| Sample_geo_accession | GSM837736
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837736/suppl/GSM837736.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837736/suppl/GSM837736.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837737 | GPL570 |
|
CL1-5_EF stimulation_biological rep1
|
lung cancer CL1-5 cell line, EF stimulation, 300mV/mm, 2hr
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-5 cells with dcEF stimulation.
|
| Sample_geo_accession | GSM837737
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837737/suppl/GSM837737.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837737/suppl/GSM837737.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837738 | GPL570 |
|
CL1-5_EF stimulation_biological rep2
|
lung cancer CL1-5 cell line, EF stimulation, 300mV/mm, 2hr
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-5 cells with dcEF stimulation.
|
| Sample_geo_accession | GSM837738
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837738/suppl/GSM837738.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837738/suppl/GSM837738.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
|
GSM837739 | GPL570 |
|
CL1-5_EF stimulation_biological rep3
|
lung cancer CL1-5 cell line, EF stimulation, 300mV/mm, 2hr
|
tumor type: non-small cell lung cancer, adenocarcinoma
|
Gene expression data from the cultured CL1-5 cells with dcEF stimulation.
|
| Sample_geo_accession | GSM837739
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Nov 21 2011
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultured cells were stimulated with the EF strength of 0 or 300mV/mm for 2 hours in serum-free medium (DMEM, Gibco). Then the cells were trypsinized and collected from the microfluidic chip. The cells were preserved in RNAlater (Ambion, Applied Biosystems) at 4C before RNA isolation.
| | Sample_growth_protocol_ch1 | Before the treatment of dcEF, the cells were cultured in a home-made microfluidic chip overnight at 37C with fresh medium flow. The microfluidic chip was made for applying stable dcEF in cell culture region. The culture medium was Dulbecco's Modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using RNaqueous kit (Ambion, Applied Biosystems) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual rev5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on GeneChip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidic Station-450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ji-Yen,,Cheng
| | Sample_contact_email | jycheng@gate.sinica.edu.tw
| | Sample_contact_department | Research Center for Applied Sciences
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837739/suppl/GSM837739.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM837nnn/GSM837739/suppl/GSM837739.CHP.gz
| | Sample_series_id | GSE33845
| | Sample_data_row_count | 54675
| | |
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