Search results for the GEO ID: GSE33984 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM840069 | GPL570 |
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EAHY non-infected control
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EAHY non-infected control
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cell type: human endothelial cell line
cell line: EAHY
infection: control
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Gene expression data from unifected EAHY cells
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Sample_geo_accession | GSM840069
| Sample_status | Public on Nov 29 2011
| Sample_submission_date | Nov 28 2011
| Sample_last_update_date | Nov 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | EAHY cells were infected with rKSHV.219 and the infected cells were selected with puromycin (1ug/ml)
| Sample_growth_protocol_ch1 | EAHY cells were grown in DMEM with 10% FCS with or without KSHV infection for 12 days.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | ombretta,,salvucci
| Sample_contact_email | salvucco@mail.nih.gov
| Sample_contact_phone | 3015949586
| Sample_contact_fax | 3015949585
| Sample_contact_department | LCO
| Sample_contact_institute | NCI
| Sample_contact_address | 37 Convent Drive
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840069/suppl/GSM840069_1.EAHY_A_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE33984
| Sample_data_row_count | 54675
| |
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GSM840070 | GPL570 |
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EAHY HSHV-infected
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EAHY KSHV-infected
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cell type: human endothelial cell line
cell line: EAHY
infection: KSHV
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Gene expression data from 12-day KSHV- infected EAHY cells
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Sample_geo_accession | GSM840070
| Sample_status | Public on Nov 29 2011
| Sample_submission_date | Nov 28 2011
| Sample_last_update_date | Nov 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | EAHY cells were infected with rKSHV.219 and the infected cells were selected with puromycin (1ug/ml)
| Sample_growth_protocol_ch1 | EAHY cells were grown in DMEM with 10% FCS with or without KSHV infection for 12 days.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | ombretta,,salvucci
| Sample_contact_email | salvucco@mail.nih.gov
| Sample_contact_phone | 3015949586
| Sample_contact_fax | 3015949585
| Sample_contact_department | LCO
| Sample_contact_institute | NCI
| Sample_contact_address | 37 Convent Drive
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840070/suppl/GSM840070_2.EAHY_B_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE33984
| Sample_data_row_count | 54675
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