Search results for the GEO ID: GSE34006  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM840332 | GPL1261 |
|
WT Tregs + vehicle 18 hr biological rep 1
|
WT Tregs + DMSO_18 hr
|
background strain: C57Bl/6
genotype/variation: wild type, A2AR+/+FoxP3GFP
age: 8-11 wk
tissue: spleen and lymph nodes
cell type: FACS-sorted CD4+CD25+FoxP3+ cells
|
Gene expression in unactivated WT Tregs
Treg WT Veh 1
|
| Sample_geo_accession | GSM840332
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Nov 29 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Vehicle (DMSO) or the adenosine 2A receptor selective agonist ATL1222 (10 nM final concentration) were added at the beginning of culture.
| | Sample_growth_protocol_ch1 | Isolated Tregs were incubated in a 5% CO2 incubator at 37C for 18 hr in RPMI 1640, 10% FBS, 10 mM HEPES, 1 mM NaPyruvate, 55 mM b-mercaptoethanol with penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Tregs using the Qiagen Rneasy kit according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip 3'-IVT Express Kit Manual, 2008-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GCOS (GeneChip Operating Software) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gilbert,R,Kinsey
| | Sample_contact_email | grk4n@virginia.edu
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | PO Box 800133
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | Virginia
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840332/suppl/GSM840332_TREGWTVEH1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840332/suppl/GSM840332_TREGWTVEH1.CHP.gz
| | Sample_series_id | GSE34006
| | Sample_data_row_count | 45101
| | |
|
GSM840333 | GPL1261 |
|
WT Tregs + vehicle 18 hr biological rep 2
|
WT Tregs + DMSO_18 hr
|
background strain: C57Bl/6
genotype/variation: wild type, A2AR+/+FoxP3GFP
age: 8-11 wk
tissue: spleen and lymph nodes
cell type: FACS-sorted CD4+CD25+FoxP3+ cells
|
Gene expression in unactivated WT Tregs
Treg WT Veh 2
|
| Sample_geo_accession | GSM840333
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Nov 29 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Vehicle (DMSO) or the adenosine 2A receptor selective agonist ATL1222 (10 nM final concentration) were added at the beginning of culture.
| | Sample_growth_protocol_ch1 | Isolated Tregs were incubated in a 5% CO2 incubator at 37C for 18 hr in RPMI 1640, 10% FBS, 10 mM HEPES, 1 mM NaPyruvate, 55 mM b-mercaptoethanol with penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Tregs using the Qiagen Rneasy kit according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip 3'-IVT Express Kit Manual, 2008-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GCOS (GeneChip Operating Software) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gilbert,R,Kinsey
| | Sample_contact_email | grk4n@virginia.edu
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | PO Box 800133
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | Virginia
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840333/suppl/GSM840333_TREGWTVEH2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840333/suppl/GSM840333_TREGWTVEH2.CHP.gz
| | Sample_series_id | GSE34006
| | Sample_data_row_count | 45101
| | |
|
GSM840334 | GPL1261 |
|
A2AR KO Tregs + vehicle 18 hr biological rep 1
|
A2AR KO Tregs + DMSO_18 hr
|
background strain: C57Bl/6
genotype/variation: A2AR knockout (KO), A2AR-/-FoxP3GFP
age: 8-11 wk
tissue: spleen and lymph nodes
cell type: FACS-sorted CD4+CD25+FoxP3+ cells
|
Gene expression in unactivated adenosine 2A receptor KO Tregs
Treg KO Veh 1
|
| Sample_geo_accession | GSM840334
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Nov 29 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Vehicle (DMSO) or the adenosine 2A receptor selective agonist ATL1222 (10 nM final concentration) were added at the beginning of culture.
| | Sample_growth_protocol_ch1 | Isolated Tregs were incubated in a 5% CO2 incubator at 37C for 18 hr in RPMI 1640, 10% FBS, 10 mM HEPES, 1 mM NaPyruvate, 55 mM b-mercaptoethanol with penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Tregs using the Qiagen Rneasy kit according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip 3'-IVT Express Kit Manual, 2008-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GCOS (GeneChip Operating Software) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gilbert,R,Kinsey
| | Sample_contact_email | grk4n@virginia.edu
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | PO Box 800133
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | Virginia
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840334/suppl/GSM840334_TREGKOVEH1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840334/suppl/GSM840334_TREGKOVEH1.CHP.gz
| | Sample_series_id | GSE34006
| | Sample_data_row_count | 45101
| | |
|
GSM840335 | GPL1261 |
|
A2AR KO Tregs + vehicle 18 hr biological rep 2
|
A2AR KO Tregs + DMSO_18 hr
|
background strain: C57Bl/6
genotype/variation: A2AR knockout (KO), A2AR-/-FoxP3GFP
age: 8-11 wk
tissue: spleen and lymph nodes
cell type: FACS-sorted CD4+CD25+FoxP3+ cells
|
Gene expression in unactivated adenosine 2A receptor KO Tregs
Treg KO Veh 2
|
| Sample_geo_accession | GSM840335
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Nov 29 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Vehicle (DMSO) or the adenosine 2A receptor selective agonist ATL1222 (10 nM final concentration) were added at the beginning of culture.
| | Sample_growth_protocol_ch1 | Isolated Tregs were incubated in a 5% CO2 incubator at 37C for 18 hr in RPMI 1640, 10% FBS, 10 mM HEPES, 1 mM NaPyruvate, 55 mM b-mercaptoethanol with penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Tregs using the Qiagen Rneasy kit according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip 3'-IVT Express Kit Manual, 2008-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GCOS (GeneChip Operating Software) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gilbert,R,Kinsey
| | Sample_contact_email | grk4n@virginia.edu
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | PO Box 800133
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | Virginia
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840335/suppl/GSM840335_TREGKOVEH2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840335/suppl/GSM840335_TREGKOVEH2.CHP.gz
| | Sample_series_id | GSE34006
| | Sample_data_row_count | 45101
| | |
|
GSM840336 | GPL1261 |
|
WT Tregs + ATL1222 18 hr biological rep 1
|
WT Tregs + ATL1222_18 hr
|
background strain: C57Bl/6
genotype/variation: wild type, A2AR+/+FoxP3GFP
age: 8-11 wk
tissue: spleen and lymph nodes
cell type: FACS-sorted CD4+CD25+FoxP3+ cells
|
Gene expression in WT Tregs after selective activation of the adenosine 2A receptor
Treg WT ATL 1
|
| Sample_geo_accession | GSM840336
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Nov 29 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Vehicle (DMSO) or the adenosine 2A receptor selective agonist ATL1222 (10 nM final concentration) were added at the beginning of culture.
| | Sample_growth_protocol_ch1 | Isolated Tregs were incubated in a 5% CO2 incubator at 37C for 18 hr in RPMI 1640, 10% FBS, 10 mM HEPES, 1 mM NaPyruvate, 55 mM b-mercaptoethanol with penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Tregs using the Qiagen Rneasy kit according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip 3'-IVT Express Kit Manual, 2008-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GCOS (GeneChip Operating Software) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gilbert,R,Kinsey
| | Sample_contact_email | grk4n@virginia.edu
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | PO Box 800133
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | Virginia
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840336/suppl/GSM840336_TREGWTATL1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840336/suppl/GSM840336_TREGWTATL1.CHP.gz
| | Sample_series_id | GSE34006
| | Sample_data_row_count | 45101
| | |
|
GSM840337 | GPL1261 |
|
WT Tregs + ATL1222 18 hr biological rep 2
|
WT Tregs + ATL1222_18 hr
|
background strain: C57Bl/6
genotype/variation: wild type, A2AR+/+FoxP3GFP
age: 8-11 wk
tissue: spleen and lymph nodes
cell type: FACS-sorted CD4+CD25+FoxP3+ cells
|
Gene expression in WT Tregs after selective activation of the adenosine 2A receptor
Treg WT ATL 2
|
| Sample_geo_accession | GSM840337
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Nov 29 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Vehicle (DMSO) or the adenosine 2A receptor selective agonist ATL1222 (10 nM final concentration) were added at the beginning of culture.
| | Sample_growth_protocol_ch1 | Isolated Tregs were incubated in a 5% CO2 incubator at 37C for 18 hr in RPMI 1640, 10% FBS, 10 mM HEPES, 1 mM NaPyruvate, 55 mM b-mercaptoethanol with penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Tregs using the Qiagen Rneasy kit according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip 3'-IVT Express Kit Manual, 2008-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GCOS (GeneChip Operating Software) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gilbert,R,Kinsey
| | Sample_contact_email | grk4n@virginia.edu
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | PO Box 800133
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | Virginia
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840337/suppl/GSM840337_TREGWTATL2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840337/suppl/GSM840337_TREGWTATL2.CHP.gz
| | Sample_series_id | GSE34006
| | Sample_data_row_count | 45101
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
