Search results for the GEO ID: GSE34042  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM840916 | GPL570 |
|
M12-5AZA-72H
|
prostate carcinoma cell lines
|
cell line: M12
treatment: 5'-Aza-2'-deoxycytidine
time: 72h
|
|
| Sample_geo_accession | GSM840916
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840916/suppl/GSM840916_M12-5AZA-72H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840917 | GPL570 |
|
M12-5AZA-96H
|
prostate carcinoma cell lines
|
cell line: M12
treatment: 5'-Aza-2'-deoxycytidine
time: 96h
|
|
| Sample_geo_accession | GSM840917
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840917/suppl/GSM840917_M12-5AZA-96H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840918 | GPL570 |
|
M12-CONT-72H
|
prostate carcinoma cell lines
|
cell line: M12
treatment: control
time: 72h
|
|
| Sample_geo_accession | GSM840918
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840918/suppl/GSM840918_M12-CONT-72H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840919 | GPL570 |
|
M12-CONT-96H
|
prostate carcinoma cell lines
|
cell line: M12
treatment: control
time: 96h
|
|
| Sample_geo_accession | GSM840919
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840919/suppl/GSM840919_M12-CONT-96H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840920 | GPL570 |
|
P69-5AZA-72H
|
prostate carcinoma cell lines
|
cell line: P69
treatment: 5'-Aza-2'-deoxycytidine
time: 72h
|
|
| Sample_geo_accession | GSM840920
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840920/suppl/GSM840920_P69-5AZA-72H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840921 | GPL570 |
|
P69-5AZA-96H
|
prostate carcinoma cell lines
|
cell line: P69
treatment: 5'-Aza-2'-deoxycytidine
time: 96h
|
|
| Sample_geo_accession | GSM840921
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840921/suppl/GSM840921_P69-5AZA-96H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840922 | GPL570 |
|
P69-CONT-72H
|
prostate carcinoma cell lines
|
cell line: P69
treatment: control
time: 72h
|
|
| Sample_geo_accession | GSM840922
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840922/suppl/GSM840922_P69-CONT-72H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840923 | GPL570 |
|
P69-CONT-96H
|
prostate carcinoma cell lines
|
cell line: P69
treatment: control
time: 96h
|
|
| Sample_geo_accession | GSM840923
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840923/suppl/GSM840923_P69-CONT-96H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840924 | GPL570 |
|
RD-5AZA-72H
|
prostate carcinoma cell lines
|
cell line: RD
treatment: 5'-Aza-2'-deoxycytidine
time: 72h
|
|
| Sample_geo_accession | GSM840924
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840924/suppl/GSM840924_RD-5AZA-72H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840925 | GPL570 |
|
RD-CONT-72H
|
prostate carcinoma cell lines
|
cell line: RD
treatment: control
time: 72h
|
|
| Sample_geo_accession | GSM840925
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840925/suppl/GSM840925_RD-CONT-72H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840926 | GPL570 |
|
Rh30-5AZA-72H
|
prostate carcinoma cell lines
|
cell line: Rh30
treatment: 5'-Aza-2'-deoxycytidine
time: 72h
|
|
| Sample_geo_accession | GSM840926
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840926/suppl/GSM840926_Rh30-5AZA-72H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
|
GSM840927 | GPL570 |
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Rh30-CONT-72H
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prostate carcinoma cell lines
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cell line: Rh30
treatment: control
time: 72h
|
|
| Sample_geo_accession | GSM840927
| | Sample_status | Public on Dec 12 2012
| | Sample_submission_date | Nov 30 2011
| | Sample_last_update_date | Dec 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with the demethylating agent 5'-Aza-2'-deoxycytidine (5-Aza) for 72 h, with daily medium changes. At the end of the incubation period the cells were harvested and total RNA was prepared for cDNA microarray analysis.
| | Sample_growth_protocol_ch1 | The P69 and M12 prostate cancer-derived cell lines were cultured in RPMI-1640 medium containing 10 ng/ml EGF, 0.1 nM dexamethasone, 5 µg/ml insulin, 5 µg/ml transferrin, and 5 ng/ml selenium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from 5-Aza-treated and untreated P69 and M12 cells using the Trizol reagent (InVitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA (15 µg) was hybridized to a GeneChip Human Genome U133 Plus 2.0 oligonucleotide arrays (HG-U133, Affymetrix, Santa Clara, CA, USA).
| | Sample_scan_protocol | Analysis was conducted with a GeneChip Scanner 3000.
| | Sample_data_processing | Differential gene expression was evaluated with the Microarray Suite 5.0 software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | jasmine,,jacob
| | Sample_contact_email | j-jacob@sheba.health.gov.il
| | Sample_contact_phone | 97235302147
| | Sample_contact_institute | sheba medical center
| | Sample_contact_address | tel hashomer
| | Sample_contact_city | ramat gan
| | Sample_contact_state | Israel
| | Sample_contact_zip/postal_code | 78995
| | Sample_contact_country | Israel
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM840nnn/GSM840927/suppl/GSM840927_Rh30-CONT-72H.CEL.gz
| | Sample_series_id | GSE34042
| | Sample_data_row_count | 54675
| | |
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