Search results for the GEO ID: GSE34114  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM842073 | GPL1261 |
|
Naïve 1 and 2h_rep1
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 2h after intraperitoneal inoculation of PBS
|
Peritoneal lavage fluid was isolated 2h after intraperitoneal inoculation of PBS
|
| Sample_geo_accession | GSM842073
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842073/suppl/GSM842073.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842074 | GPL1261 |
|
Naïve 1 and 2h_rep2
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 2h after intraperitoneal inoculation of PBS
|
Peritoneal lavage fluid was isolated 2h after intraperitoneal inoculation of PBS
|
| Sample_geo_accession | GSM842074
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842074/suppl/GSM842074.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842075 | GPL1261 |
|
Naïve 18h_rep1
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of PBS
|
Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of PBS
|
| Sample_geo_accession | GSM842075
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842075/suppl/GSM842075.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842076 | GPL1261 |
|
Naïve 18h_rep2
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of PBS
|
Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of PBS
|
| Sample_geo_accession | GSM842076
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842076/suppl/GSM842076.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842077 | GPL1261 |
|
Naïve 18h_rep3
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of PBS
|
Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of PBS
|
| Sample_geo_accession | GSM842077
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842077/suppl/GSM842077.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842078 | GPL1261 |
|
E. coli 1h_rep1
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 1h after intraperitoneal inoculation of E. coli
|
Peritoneal lavage fluid was isolated 1h after intraperitoneal inoculation of E. coli
|
| Sample_geo_accession | GSM842078
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842078/suppl/GSM842078.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842079 | GPL1261 |
|
E. coli 1h_rep2
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 1h after intraperitoneal inoculation of E. coli
|
Peritoneal lavage fluid was isolated 1h after intraperitoneal inoculation of E. coli
|
| Sample_geo_accession | GSM842079
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842079/suppl/GSM842079.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842080 | GPL1261 |
|
E. coli 2h_rep1
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 2h after intraperitoneal inoculation of E. coli
|
Peritoneal lavage fluid was isolated 2h after intraperitoneal inoculation of E. coli
|
| Sample_geo_accession | GSM842080
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842080/suppl/GSM842080.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842081 | GPL1261 |
|
E. coli 2h_rep2
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 2h after intraperitoneal inoculation of E. coli
|
Peritoneal lavage fluid was isolated 2h after intraperitoneal inoculation of E. coli
|
| Sample_geo_accession | GSM842081
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842081/suppl/GSM842081.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842082 | GPL1261 |
|
E. coli 18h_rep1
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 1h after intraperitoneal inoculation of E. coli
|
Peritoneal lavage fluid was isolated 1h after intraperitoneal inoculation of E. coli
|
| Sample_geo_accession | GSM842082
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842082/suppl/GSM842082.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
| | |
|
GSM842083 | GPL1261 |
|
E. coli 18h_rep2
|
Peritoneal cells
|
gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of E. coli
|
Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of E. coli
|
| Sample_geo_accession | GSM842083
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842083/suppl/GSM842083.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
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GSM842084 | GPL1261 |
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E. coli 18h_rep3
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Peritoneal cells
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gender: female
strain: C57Bl/6 x C3H F1
treatment group: Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of E. coli
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Peritoneal lavage fluid was isolated 18h after intraperitoneal inoculation of E. coli
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| Sample_geo_accession | GSM842084
| | Sample_status | Public on Dec 30 2012
| | Sample_submission_date | Dec 02 2011
| | Sample_last_update_date | Dec 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Each mice in the E. coli infected group was injected with a 2X108 cells of a non-pathogenic strain of E. coli intraperitoneally, the control group was injected with an equal amount of PBS. Peritonal lavage fluid was obtained from each mice 1, 2 and 18 h after infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from peritoneal cells using TRIzol (Invitrogen, Stockholm, Sweden) protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 µg total RNA using the GeneChip IVT Labeling Kit (Affymetrix) Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 7.5 ug total RNA using the GeneChip IVT Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45° C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Data was normalized by using the Robust Multichip Average (RMA) protocol implemented in Genesifter (Geospiza, Seattle, WA)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Minny,,Bhatty
| | Sample_contact_institute | Mississippi State University
| | Sample_contact_address | 240 Wise Center Dr, Mississippi State
| | Sample_contact_city | Starkville
| | Sample_contact_state | Mississippi
| | Sample_contact_zip/postal_code | 39762
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842084/suppl/GSM842084.CEL.gz
| | Sample_series_id | GSE34114
| | Sample_data_row_count | 45101
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