Search results for the GEO ID: GSE34146  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM842874 | GPL1261 |
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8534_Untreated_24hr_1
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mouse mammary tumor derived cell line
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strain: FVB/N;B6
gender: female
tissue: Primary mammary tumor
cell line: mouse mammary tumor derived cell line 8534
agent: Untreated
intracellular domain of notch1 (icn1) status: On
|
8534 Untreated.cel
|
| Sample_geo_accession | GSM842874
| | Sample_status | Public on Dec 05 2011
| | Sample_submission_date | Dec 05 2011
| | Sample_last_update_date | Dec 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were left untreated or treated with 2 ug/ml doxycycline for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were maintained in DMEM/F12 1:1 media supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, penicillin (100 U/ml) and streptomycin (100 µg/ml). Cells were maintained at 37°C in an atmosphere of 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by scraping and total RNA was isolated using Trizol (Invitrogen), followed by further purifification using the RNAeasy Mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were processed and labeled with the Affymetrix 3’ IVT Express Kit with biotin according to the standard Affymetrix protocol from 5ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Affymetrix Mouse Genome 430A_2.0 chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| | Sample_data_processing | Analyses were performed using BRB-ArrayTools developed by Dr. Richard Simon and BRB-ArrayTools Development Team.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matthew,J,Simmons
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | UMASS Medical School
| | Sample_contact_address | 364 Plantation St
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842874/suppl/GSM842874.CEL.gz
| | Sample_series_id | GSE34146
| | Sample_data_row_count | 45101
| | |
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GSM842875 | GPL1261 |
|
8534_Dox_24hr_1
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mouse mammary tumor derived cell line
|
strain: FVB/N;B6
gender: female
tissue: Primary mammary tumor
cell line: mouse mammary tumor derived cell line 8534
agent: doxycycline
intracellular domain of notch1 (icn1) status: Off
|
8534 Dox.cel
|
| Sample_geo_accession | GSM842875
| | Sample_status | Public on Dec 05 2011
| | Sample_submission_date | Dec 05 2011
| | Sample_last_update_date | Dec 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were left untreated or treated with 2 ug/ml doxycycline for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were maintained in DMEM/F12 1:1 media supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, penicillin (100 U/ml) and streptomycin (100 µg/ml). Cells were maintained at 37°C in an atmosphere of 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by scraping and total RNA was isolated using Trizol (Invitrogen), followed by further purifification using the RNAeasy Mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were processed and labeled with the Affymetrix 3’ IVT Express Kit with biotin according to the standard Affymetrix protocol from 5ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Affymetrix Mouse Genome 430A_2.0 chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| | Sample_data_processing | Analyses were performed using BRB-ArrayTools developed by Dr. Richard Simon and BRB-ArrayTools Development Team.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matthew,J,Simmons
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | UMASS Medical School
| | Sample_contact_address | 364 Plantation St
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842875/suppl/GSM842875.CEL.gz
| | Sample_series_id | GSE34146
| | Sample_data_row_count | 45101
| | |
|
GSM842876 | GPL1261 |
|
8542_Untreated_24hr_1
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mouse mammary tumor derived cell line
|
strain: FVB/N;B6
gender: female
tissue: Primary mammary tumor
cell line: mouse mammary tumor derived cell line 8542
agent: Untreated
intracellular domain of notch1 (icn1) status: On
|
8542 Untreated.cel
|
| Sample_geo_accession | GSM842876
| | Sample_status | Public on Dec 05 2011
| | Sample_submission_date | Dec 05 2011
| | Sample_last_update_date | Dec 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were left untreated or treated with 2 ug/ml doxycycline for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were maintained in DMEM/F12 1:1 media supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, penicillin (100 U/ml) and streptomycin (100 µg/ml). Cells were maintained at 37°C in an atmosphere of 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by scraping and total RNA was isolated using Trizol (Invitrogen), followed by further purifification using the RNAeasy Mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were processed and labeled with the Affymetrix 3’ IVT Express Kit with biotin according to the standard Affymetrix protocol from 5ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Affymetrix Mouse Genome 430A_2.0 chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| | Sample_data_processing | Analyses were performed using BRB-ArrayTools developed by Dr. Richard Simon and BRB-ArrayTools Development Team.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matthew,J,Simmons
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | UMASS Medical School
| | Sample_contact_address | 364 Plantation St
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842876/suppl/GSM842876.CEL.gz
| | Sample_series_id | GSE34146
| | Sample_data_row_count | 45101
| | |
|
GSM842877 | GPL1261 |
|
8542_Dox_24hr_1
|
mouse mammary tumor derived cell line
|
strain: FVB/N;B6
gender: female
tissue: Primary mammary tumor
cell line: mouse mammary tumor derived cell line 8542
agent: doxycycline
intracellular domain of notch1 (icn1) status: Off
|
8542 Dox.cel
|
| Sample_geo_accession | GSM842877
| | Sample_status | Public on Dec 05 2011
| | Sample_submission_date | Dec 05 2011
| | Sample_last_update_date | Dec 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were left untreated or treated with 2 ug/ml doxycycline for 24 hours.
| | Sample_growth_protocol_ch1 | Cells were maintained in DMEM/F12 1:1 media supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, penicillin (100 U/ml) and streptomycin (100 µg/ml). Cells were maintained at 37°C in an atmosphere of 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected by scraping and total RNA was isolated using Trizol (Invitrogen), followed by further purifification using the RNAeasy Mini kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were processed and labeled with the Affymetrix 3’ IVT Express Kit with biotin according to the standard Affymetrix protocol from 5ug of total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Affymetrix Mouse Genome 430A_2.0 chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| | Sample_data_processing | Analyses were performed using BRB-ArrayTools developed by Dr. Richard Simon and BRB-ArrayTools Development Team.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matthew,J,Simmons
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | UMASS Medical School
| | Sample_contact_address | 364 Plantation St
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM842nnn/GSM842877/suppl/GSM842877.CEL.gz
| | Sample_series_id | GSE34146
| | Sample_data_row_count | 45101
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