Search results for the GEO ID: GSE34268 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM875664 | GPL96 |
|
normal erythroid sample 1 Day 7
|
normal erythroid Day 7
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875664
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875664/suppl/GSM875664_N1_D7.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875665 | GPL96 |
|
normal erythroid sample 1 Day 10
|
normal erythroid Day 10
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875665
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875665/suppl/GSM875665_N1_D10.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875666 | GPL96 |
|
normal erythroid sample 1 Day 14
|
normal erythroid Day 14
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875666
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875666/suppl/GSM875666_N1_D14.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875667 | GPL96 |
|
normal erythroid sample 2 Day 7
|
normal erythroid Day 7
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875667
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875667/suppl/GSM875667_N2_D7.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875668 | GPL96 |
|
normal erythroid sample 3 Day 7
|
normal erythroid Day 7
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875668
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875668/suppl/GSM875668_N3_D7.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875669 | GPL96 |
|
normal erythroid sample 3 Day 10
|
normal erythroid Day 10
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875669
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875669/suppl/GSM875669_N3_D10.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875670 | GPL96 |
|
normal erythroid sample 3 Day 14
|
normal erythroid Day 14
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875670
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875670/suppl/GSM875670_N3_D14.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875671 | GPL96 |
|
normal erythroid sample 4 Day 10
|
normal erythroid Day 10
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875671
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875671/suppl/GSM875671_N4_D10.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875672 | GPL96 |
|
normal erythroid sample 4 Day 14
|
normal erythroid Day 14
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875672
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875672/suppl/GSM875672_N4_D14.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875673 | GPL96 |
|
normal erythroid sample 5 Day 10
|
normal erythroid Day 10
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875673
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875673/suppl/GSM875673_N5_D10.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875674 | GPL96 |
|
normal erythroid sample 5 Day 14
|
normal erythroid Day 14
|
cell type: normal erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875674
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875674/suppl/GSM875674_N5_D14.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875675 | GPL96 |
|
MDS erythroid sample 1 Day 7
|
MDS erythroid Day 7
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875675
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875675/suppl/GSM875675_MDS1_D7.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875676 | GPL96 |
|
MDS erythroid sample 1 Day 10
|
MDS erythroid Day 10
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875676
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875676/suppl/GSM875676_MDS1_D10.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875677 | GPL96 |
|
MDS erythroid sample 1 Day 14
|
MDS erythroid Day 14
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875677
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875677/suppl/GSM875677_MDS1_D14.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875678 | GPL96 |
|
MDS erythroid sample 2 Day 7
|
MDS erythroid Day 7
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875678
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875678/suppl/GSM875678_MDS2_D7.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875679 | GPL96 |
|
MDS erythroid sample 2 Day 10
|
MDS erythroid Day 10
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875679
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875679/suppl/GSM875679_MDS2_D10.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875680 | GPL96 |
|
MDS erythroid sample 2 Day 14
|
MDS erythroid Day 14
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875680
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875680/suppl/GSM875680_MDS2_D14.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875681 | GPL96 |
|
MDS erythroid sample 3 Day 7
|
MDS erythroid Day 7
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875681
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875681/suppl/GSM875681_MDS3_D7.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875682 | GPL96 |
|
MDS erythroid sample 3 Day 10
|
MDS erythroid Day 10
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875682
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875682/suppl/GSM875682_MDS3_D10.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875683 | GPL96 |
|
MDS erythroid sample 3 Day 14
|
MDS erythroid Day 14
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875683
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875683/suppl/GSM875683_MDS3_D14.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875684 | GPL96 |
|
MDS erythroid sample 4 Day 7
|
MDS erythroid Day 7
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875684
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875684/suppl/GSM875684_MDS4_D7.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875685 | GPL96 |
|
MDS erythroid sample 4 Day 10
|
MDS erythroid Day 10
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875685
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875685/suppl/GSM875685_MDS4_D10.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875686 | GPL96 |
|
MDS erythroid sample 4 Day 14
|
MDS erythroid Day 14
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875686
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875686/suppl/GSM875686_MDS4_D14.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
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GSM875687 | GPL96 |
|
MDS erythroid sample 5 Day 7
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MDS erythroid Day 7
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cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875687
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875687/suppl/GSM875687_MDS5_D7.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875688 | GPL96 |
|
MDS erythroid sample 5 Day 10
|
MDS erythroid Day 10
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875688
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875688/suppl/GSM875688_MDS5_D10.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
|
GSM875689 | GPL96 |
|
MDS erythroid sample 5 Day 14
|
MDS erythroid Day 14
|
cell type: MDS erythroid
|
Gene expression data from erythroid cell
|
Sample_geo_accession | GSM875689
| Sample_status | Public on Feb 14 2012
| Sample_submission_date | Feb 14 2012
| Sample_last_update_date | Feb 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Erythroids cells at the appropriate day were collected, whashed twice in PBS and placed on ice in the Trizol solution.
| Sample_growth_protocol_ch1 | CD34+ cells were in erythroid conditions in IMDM-BIT medium added with Epo, SCF, DXM and IGF1 during 10 days then into Epo and Insuline only during 4 days
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocols
| Sample_hyb_protocol | standard Affymetrix protocols
| Sample_scan_protocol | standard Affymetrix protocols
| Sample_data_processing | Image data were quantified with Microarray Software Suite Version 5.0 (Affymetrix). Data from 10820_at labeled probes were normalized to median of 3 replicates by GC-Robust MultiChip Analysis and significance analysis of microarrays used for marker selection was performed in the software R. Neighborhood analysis was performed using the published methods. Genes differentially expressed (ANOVA test; P<.01) were identified by hierarchic clustering using the GeneSpring Version 6.0 software (Silicon Genetics).
| Sample_platform_id | GPL96
| Sample_contact_name | olivier,,kosmider
| Sample_contact_email | olivier.kosmider@cch.aphp.fr
| Sample_contact_phone | +33158412002
| Sample_contact_fax | +33158412006
| Sample_contact_institute | Hopital Cochin
| Sample_contact_address | 27 Rue du faubourg St Jacques
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75014
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM875nnn/GSM875689/suppl/GSM875689_MDS5_D14.CEL.gz
| Sample_series_id | GSE34268
| Sample_data_row_count | 22283
| |
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