Search results for the GEO ID: GSE34324 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM847170 | GPL1261 |
|
[mDCs]_[control]_[1]
|
mDCs_control
|
strain: BALB/c
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847170
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol and Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847170/suppl/GSM847170_002590_3_DC.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847170/suppl/GSM847170_002590_3_DC.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847191 | GPL1261 |
|
[mDCs]_[RA]_[1]
|
mDCs_Retinoic Acid
|
strain: BALB/c
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847191
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | All-trans Retinoic Acid added 10nmol/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol and Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847191/suppl/GSM847191_002592_3_RA.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847191/suppl/GSM847191_002592_3_RA.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847192 | GPL1261 |
|
[mDCs]_[Ro]_[1]
|
mDCs_Ro41-5253
|
strain: BALB/c
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847192
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Ro41-5253 added 100nmol/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol and Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847192/suppl/GSM847192_002594_3_RO.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847192/suppl/GSM847192_002594_3_RO.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847193 | GPL1261 |
|
[mDCs]_[control+LPS]_[1]
|
mDCs_LPS
|
strain: BALB/c
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847193
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LPS added at 1ug/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol and Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847193/suppl/GSM847193_002591_3_DC_LPS.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847193/suppl/GSM847193_002591_3_DC_LPS.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847194 | GPL1261 |
|
[mDCs]_[RA+LPS]_[1]
|
mDCs_Retinoic Acid_LPS
|
strain: BALB/c
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847194
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LPS added at 1ug/ml
| Sample_treatment_protocol_ch1 | All-trans Retinoic Acid added 10nmol/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol and Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847194/suppl/GSM847194_002593_3_RA_LPS.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847194/suppl/GSM847194_002593_3_RA_LPS.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847195 | GPL1261 |
|
[mDCs]_[Ro+LPS]_[1]
|
mDCs_Ro41-5253_LPS
|
strain: BALB/c
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847195
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LPS added at 1ug/ml
| Sample_treatment_protocol_ch1 | Ro41-5253 added 100nmol/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol and Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847195/suppl/GSM847195_002595_3_RO_LPS.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847195/suppl/GSM847195_002595_3_RO_LPS.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847211 | GPL1261 |
|
[mDCs]_[control]_[2]
|
mDCs_control
|
strain: BL6
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847211
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847211/suppl/GSM847211_002989_1m_DC.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847211/suppl/GSM847211_002989_1m_DC.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847217 | GPL1261 |
|
[mDCs]_[RA]_[2]
|
mDCs_Retinoic Acid
|
strain: BL6
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847217
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | All-trans Retinoic Acid added 10nmol/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847217/suppl/GSM847217_002990_2m_DC_RA.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847217/suppl/GSM847217_002990_2m_DC_RA.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847218 | GPL1261 |
|
[mDCs]_[Ro]_[2]
|
mDCs_Ro41-5253
|
strain: BL6
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847218
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Ro41-5253 added 100nmol/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847218/suppl/GSM847218_002991_3m_DC_RO.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847218/suppl/GSM847218_002991_3m_DC_RO.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847219 | GPL1261 |
|
[mDCs]_[control+LPS]_[2]
|
mDCs_LPS
|
strain: BL6
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847219
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LPS added at 1ug/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847219/suppl/GSM847219_002992_4m_LPS.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847219/suppl/GSM847219_002992_4m_LPS.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
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GSM847220 | GPL1261 |
|
[mDCs]_[RA+LPS]_[2]
|
mDCs_Retinoic Acid_LPS
|
strain: BL6
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847220
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LPS added at 1ug/ml
| Sample_treatment_protocol_ch1 | All-trans Retinoic Acid added 10nmol/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847220/suppl/GSM847220_002993_5m_LPS_RA.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847220/suppl/GSM847220_002993_5m_LPS_RA.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
GSM847221 | GPL1261 |
|
[mDCs]_[Ro+LPS]_[2]
|
mDCs_Ro41-5253_LPS
|
strain: BL6
age: 6-8 weeks
tissue: bone marrow
cell type: dendritic cells
|
|
Sample_geo_accession | GSM847221
| Sample_status | Public on Feb 26 2013
| Sample_submission_date | Dec 09 2011
| Sample_last_update_date | Feb 26 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LPS added at 1ug/ml
| Sample_treatment_protocol_ch1 | Ro41-5253 added 100nmol/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol Qiagen Total RNA isolation kit
| Sample_label_ch1 | Biotin (as a biotinylated nucleotide)
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 ug total RNA according to the Single Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences Intl. Plymouth Meeting, PA).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized with a GeneChip Hybridization Oven 640 for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400 with fludics protocol EukGE_WS2v5 and user prepared solutions, as per protocols provided by Affymetrix in the GeneChip Expression Analysis Technical Manual, 2004. This included Enzo Life Sciences' BioArray Eukaryotic Hybridization Controls.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G enabled forHigh-Resolution Scanning. The instruments were controlled using GCOS version1.4 software.
| Sample_data_processing | The data were condensed with MAS 5.0 using the GeneChip Operation Software.
| Sample_platform_id | GPL1261
| Sample_contact_name | chang,,Kim
| Sample_contact_email | chkimpurdue@gmail.com
| Sample_contact_phone | 765-494-0976
| Sample_contact_fax | 765-494-9830
| Sample_contact_laboratory | Immunology and Hematopoiesis
| Sample_contact_department | Comparative Pathobiology
| Sample_contact_institute | Purdue
| Sample_contact_address | 725 Harrison St
| Sample_contact_city | West Lafayette
| Sample_contact_state | IN
| Sample_contact_zip/postal_code | 47907
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847221/suppl/GSM847221_002994_6m_LPS_RO.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847221/suppl/GSM847221_002994_6m_LPS_RO.CHP.gz
| Sample_series_id | GSE34324
| Sample_data_row_count | 45101
| |
|
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