Search results for the GEO ID: GSE34351  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM847661 | GPL1261 |
|
B10 ischemic kidney, biological rep1
|
WT ischemic kidney
|
gender: Male
strain: C57BL/10
genotype: WT
tissue: Kidney
surgery: Ischemic
reperfusion time: 4hr
|
Gene expression data from WT ischemic kidney(23min) at 4hr reperfusion(No.1)
|
| Sample_geo_accession | GSM847661
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847661/suppl/GSM847661.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847661/suppl/GSM847661.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847662 | GPL1261 |
|
B10 ischemic kidney, biological rep2
|
WT ischemic kidney
|
gender: Male
strain: C57BL/10
genotype: WT
tissue: Kidney
surgery: Ischemic
reperfusion time: 4hr
|
Gene expression data from WT ischemic kidney(23min) at 4hr reperfusion(No.2)
|
| Sample_geo_accession | GSM847662
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847662/suppl/GSM847662.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847662/suppl/GSM847662.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847663 | GPL1261 |
|
B10 ischemic kidney, biological rep3
|
WT ischemic kidney
|
gender: Male
strain: C57BL/10
genotype: WT
tissue: Kidney
surgery: Ischemic
reperfusion time: 4hr
|
Gene expression data from WT ischemic kidney(23min) at 4hr reperfusion(No.3)
|
| Sample_geo_accession | GSM847663
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847663/suppl/GSM847663.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847663/suppl/GSM847663.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847664 | GPL1261 |
|
B10 sham kidney, biological rep1
|
WT sham kidney
|
gender: Male
strain: C57BL/10
genotype: WT
tissue: Kidney
surgery: Sham
reperfusion time: 4hr
|
Gene expression data from WT sham kidney at 4hr reperfusion(No.1)
|
| Sample_geo_accession | GSM847664
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847664/suppl/GSM847664.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847664/suppl/GSM847664.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847665 | GPL1261 |
|
B10 sham kidney, biological rep2
|
WT sham kidney
|
gender: Male
strain: C57BL/10
genotype: WT
tissue: Kidney
surgery: Sham
reperfusion time: 4hr
|
Gene expression data from WT sham kidney at 4hr reperfusion(No.2)
|
| Sample_geo_accession | GSM847665
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847665/suppl/GSM847665.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847665/suppl/GSM847665.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847666 | GPL1261 |
|
B10 sham kidney, biological rep3
|
WT sham kidney
|
gender: Male
strain: C57BL/10
genotype: WT
tissue: Kidney
surgery: Sham
reperfusion time: 4hr
|
Gene expression data from WT sham kidney at 4hr reperfusion(No.3)
|
| Sample_geo_accession | GSM847666
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847666/suppl/GSM847666.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847666/suppl/GSM847666.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847667 | GPL1261 |
|
TLR4 null ischemic kidney, biological rep1
|
TLR4 null ischemic kidney
|
gender: Male
strain: C57BL/10ScNJ
genotype: TLR4-/-
tissue: Kidney
surgery: Ischemic
reperfusion time: 4hr
|
Gene expression data from TLR4-/- ischemic(23min) kidney at 4hr reperfusion(No.1)
|
| Sample_geo_accession | GSM847667
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847667/suppl/GSM847667.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847667/suppl/GSM847667.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847668 | GPL1261 |
|
TLR4 null ischemic kidney, biological rep2
|
TLR4 null ischemic kidney
|
gender: Male
strain: C57BL/10ScNJ
genotype: TLR4-/-
tissue: Kidney
surgery: Ischemic
reperfusion time: 4hr
|
Gene expression data from TLR4-/- ischemic(23min) kidney at 4hr reperfusion(No.2)
|
| Sample_geo_accession | GSM847668
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847668/suppl/GSM847668.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847668/suppl/GSM847668.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847669 | GPL1261 |
|
TLR4 null ischemic kidney, biological rep3
|
TLR4 null ischemic kidney
|
gender: Male
strain: C57BL/10ScNJ
genotype: TLR4-/-
tissue: Kidney
surgery: Ischemic
reperfusion time: 4hr
|
Gene expression data from TLR4-/- ischemic(23min) kidney at 4hr reperfusion(No.3)
|
| Sample_geo_accession | GSM847669
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847669/suppl/GSM847669.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847669/suppl/GSM847669.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847670 | GPL1261 |
|
TLR4 null sham kidney, biological rep1
|
TLR4 null sham kidney
|
gender: Male
strain: C57BL/10ScNJ
genotype: TLR4-/-
tissue: Kidney
surgery: Sham
reperfusion time: 4hr
|
Gene expression data from TLR4-/- sham kidney at 4hr reperfusion(No.1)
|
| Sample_geo_accession | GSM847670
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847670/suppl/GSM847670.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847670/suppl/GSM847670.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847671 | GPL1261 |
|
TLR4 null sham kidney, biological rep2
|
TLR4 null sham kidney
|
gender: Male
strain: C57BL/10ScNJ
genotype: TLR4-/-
tissue: Kidney
surgery: Sham
reperfusion time: 4hr
|
Gene expression data from TLR4-/- sham kidney at 4hr reperfusion(No.2)
|
| Sample_geo_accession | GSM847671
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847671/suppl/GSM847671.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847671/suppl/GSM847671.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
|
GSM847672 | GPL1261 |
|
TLR4 null sham kidney, biological rep3
|
TLR4 null sham kidney
|
gender: Male
strain: C57BL/10ScNJ
genotype: TLR4-/-
tissue: Kidney
surgery: Sham
reperfusion time: 4hr
|
Gene expression data from TLR4-/- sham kidney at 4hr reperfusion(No.3)
|
| Sample_geo_accession | GSM847672
| | Sample_status | Public on Aug 15 2012
| | Sample_submission_date | Dec 12 2011
| | Sample_last_update_date | Aug 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were placed under inhaled isofluorane with body temperature maintained at 37°C. After right nephrectomy, the left pedicle was occluded with a microaneurysm clip for 23min. The clip was then released and buprenorphine was given S.C.. Sham mice underwent the same right nephrectomy except the clip was placed underneath the pedicle. Kidneys were harvested at 4hr reperfusion.
| | Sample_growth_protocol_ch1 | 8 wks old male mice were purchased from the Jackson Laboratory and fed ad libitum in our animal facility before surgery.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidneys were dissected, snap-frozen in liquid nitrogen and stored at -80°C. Samples were homogenized and total RNA was extracted using the the RNeasy Midi Kit(Qiagen) according to the manufacturer's manual. RNA integrity was measured on the Agilent 2100 Bioanalyzer(RIN:9.5).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was amplified from 1µg of total RNA using the MessageAmp II-Biotin Enhanced Kit(Ambion) protocol.
| | Sample_hyb_protocol | Following fragmentation, 15µg of cRNA were hybridized at 45°C for 16 hr on the GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0(MAS5.0) using Affymetrix default analysis and global scaling as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | JIANLIN,,CHEN
| | Sample_contact_email | jianlin.chen@utsouthwestern.edu
| | Sample_contact_phone | 2146486785
| | Sample_contact_fax | 2146482071
| | Sample_contact_laboratory | Lu Lab
| | Sample_contact_department | Nephrology/Internal Medicine
| | Sample_contact_institute | University of Texas Southwestern Medical Center at Dallas
| | Sample_contact_address | 5323 HARY HINES BLVD.
| | Sample_contact_city | DALLAS
| | Sample_contact_state | TEXAS
| | Sample_contact_zip/postal_code | 75390
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847672/suppl/GSM847672.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM847nnn/GSM847672/suppl/GSM847672.CHP.gz
| | Sample_series_id | GSE34351
| | Sample_data_row_count | 45101
| | |
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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