Search results for the GEO ID: GSE34424  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM848858 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 1, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848858
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848858/suppl/GSM848858.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848859 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 2, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848859
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848859/suppl/GSM848859.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848860 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 3, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848860
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848860/suppl/GSM848860.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848861 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 4, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848861
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848861/suppl/GSM848861.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848862 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 5, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848862
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848862/suppl/GSM848862.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848863 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 17, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848863
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848863/suppl/GSM848863.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848864 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 6, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848864
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848864/suppl/GSM848864.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848865 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 7, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848865
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848865/suppl/GSM848865.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848866 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 8, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848866
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848866/suppl/GSM848866.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848867 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 9, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848867
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848867/suppl/GSM848867.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848868 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 10, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848868
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848868/suppl/GSM848868.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848869 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 11, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848869
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848869/suppl/GSM848869.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848870 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 12, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848870
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848870/suppl/GSM848870.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848871 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 13, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848871
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848871/suppl/GSM848871.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848872 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 14, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848872
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848872/suppl/GSM848872.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848873 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 15, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848873
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848873/suppl/GSM848873.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848874 | GPL85 |
|
rat hippocampus (CA1 region), home cage, subject 16, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: home cage (HC)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848874
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | home cage (HC)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848874/suppl/GSM848874.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848875 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 18, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848875
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848875/suppl/GSM848875.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848876 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 19, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848876
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848876/suppl/GSM848876.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848877 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 20, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848877
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848877/suppl/GSM848877.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848878 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 21, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848878
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848878/suppl/GSM848878.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848879 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 22, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848879
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848879/suppl/GSM848879.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848880 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 23, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848880
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848880/suppl/GSM848880.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848881 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 24, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848881
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848881/suppl/GSM848881.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848882 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 25, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848882
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848882/suppl/GSM848882.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848883 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 28, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848883
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848883/suppl/GSM848883.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848884 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 29, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848884
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848884/suppl/GSM848884.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848885 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 30, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848885
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848885/suppl/GSM848885.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848886 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 31, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848886
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848886/suppl/GSM848886.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848887 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 32, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848887
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848887/suppl/GSM848887.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848888 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 33, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848888
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848888/suppl/GSM848888.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848889 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 34, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848889
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848889/suppl/GSM848889.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848890 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours sleep deprive, subject 35, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours sleep deprived (24SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848890
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours sleep deprived (24SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848890/suppl/GSM848890.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848891 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 36, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848891
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848891/suppl/GSM848891.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848892 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 37, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848892
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848892/suppl/GSM848892.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848893 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 38, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848893
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848893/suppl/GSM848893.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848894 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 39, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848894
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848894/suppl/GSM848894.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848895 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 40, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848895
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848895/suppl/GSM848895.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848896 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 41, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848896
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848896/suppl/GSM848896.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848897 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 42, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848897
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848897/suppl/GSM848897.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848898 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 43, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848898
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848898/suppl/GSM848898.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848899 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 46, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848899
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848899/suppl/GSM848899.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848900 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 47, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848900
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848900/suppl/GSM848900.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848901 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 48, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848901
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848901/suppl/GSM848901.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848902 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 49, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848902
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848902/suppl/GSM848902.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848903 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 50, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848903
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848903/suppl/GSM848903.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848904 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 51, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848904
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848904/suppl/GSM848904.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848905 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 52, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848905
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848905/suppl/GSM848905.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848906 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 53, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848906
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848906/suppl/GSM848906.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848907 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 54, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848907
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848907/suppl/GSM848907.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848908 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 55, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848908
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848908/suppl/GSM848908.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848909 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 56, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848909
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848909/suppl/GSM848909.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848910 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours sleep deprive, subject 57, cohort 2
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours sleep deprived (72SD)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848910
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours sleep deprived (72SD)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848910/suppl/GSM848910.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848911 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 58, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848911
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848911/suppl/GSM848911.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848912 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 59, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848912
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848912/suppl/GSM848912.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848913 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 60, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848913
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848913/suppl/GSM848913.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848914 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 61, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848914
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848914/suppl/GSM848914.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848915 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 62, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848915
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848915/suppl/GSM848915.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848916 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 63, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848916
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848916/suppl/GSM848916.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848917 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 64, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848917
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848917/suppl/GSM848917.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848918 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 65, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848918
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848918/suppl/GSM848918.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848919 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 66, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848919
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848919/suppl/GSM848919.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848920 | GPL85 |
|
rat hippocampus (CA1 region), 24 hours novel environment stress, subject 67, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 24 hours novel environment stress (24NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848920
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 24 hours novel environment stress (24NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848920/suppl/GSM848920.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848921 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours novel environment stress, subject 68, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours novel environment stress (72NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848921
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours novel environment stress (72NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848921/suppl/GSM848921.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848922 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours novel environment stress, subject 69, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours novel environment stress (72NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848922
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours novel environment stress (72NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848922/suppl/GSM848922.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848923 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours novel environment stress, subject 70, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours novel environment stress (72NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848923
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours novel environment stress (72NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848923/suppl/GSM848923.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848924 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours novel environment stress, subject 71, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours novel environment stress (72NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848924
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours novel environment stress (72NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848924/suppl/GSM848924.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848925 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours novel environment stress, subject 72, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours novel environment stress (72NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848925
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours novel environment stress (72NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848925/suppl/GSM848925.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
|
GSM848926 | GPL85 |
|
rat hippocampus (CA1 region), 72 hours novel environment stress, subject 73, cohort 1
|
hippocampus (CA1 region)
|
gender: Male
strain: Fisher 344
treatment: 72 hours novel environment stress (72NES)
|
hippocampus CA1 region
|
| Sample_geo_accession | GSM848926
| | Sample_status | Public on Jul 17 2012
| | Sample_submission_date | Dec 13 2011
| | Sample_last_update_date | Jul 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | 72 hours novel environment stress (72NES)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, 20 ug of biotin-labeled cRNA was generated from 5ug of total RNA according to manufacturer’s instructions (Affymetrix).
| | Sample_hyb_protocol | 20ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
| | Sample_scan_protocol | Standard Affymetrix
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL85
| | Sample_contact_name | Eric,M,Blalock
| | Sample_contact_email | emblal@uky.edu
| | Sample_contact_phone | 859-323-8033
| | Sample_contact_laboratory | Blalock
| | Sample_contact_department | Molecular and Biomedical Pharmacology
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St.
| | Sample_contact_city | Lexington
| | Sample_contact_state | KY
| | Sample_contact_zip/postal_code | 40475
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848926/suppl/GSM848926.CEL.gz
| | Sample_series_id | GSE34424
| | Sample_data_row_count | 8799
| | |
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