Sample_geo_accession | GSM848955
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Sample_status | Public on Dec 15 2011
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Sample_submission_date | Dec 14 2011
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Sample_last_update_date | Dec 15 2011
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Homo sapiens
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Sample_taxid_ch1 | 9606
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Sample_treatment_protocol_ch1 | No treatment. Logarithmically growing cells were collected for RNA extraction.
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Sample_growth_protocol_ch1 | hTERT immortalized hMEC stem/progenitor and myoepithelial cells were grown in the DFCI-1, and MEGM medium
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix 3' IVT Express protocol from 200 ng total RNA.
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Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 +2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
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Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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Sample_platform_id | GPL570
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Sample_contact_name | Gautam,,Malhotra
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Sample_contact_institute | UNMC
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Sample_contact_address | 985805 Nebraska Medical Center
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Sample_contact_city | Omaha
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Sample_contact_state | NE
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Sample_contact_zip/postal_code | 68198-5805
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Sample_contact_country | USA
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848955/suppl/GSM848955.CEL.gz
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM848nnn/GSM848955/suppl/GSM848955.CHP.gz
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Sample_series_id | GSE34440
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Sample_data_row_count | 54675
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