Search results for the GEO ID: GSE34511 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM850394 | GPL1261 |
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Wild-type ventral prostates
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3-month-old male mouse, ventral prostate
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tissue: ventral prostate
genotype: U19/Eaf2 +/+
genetic background: C57BL/6J
age: 3-month-old
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Gene expression data from ventral prostate
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Sample_geo_accession | GSM850394
| Sample_status | Public on Dec 17 2011
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Dec 17 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed following RNeasy Mini Kit QIAGEN Cat # 74104 according to the manufacturer's instructions. RNA quality and quantity were measured with spectrophotometry.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using MEGAscriptTM system according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15-20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the HP ChipScanner (Affymetrix).
| Sample_data_processing | The data were analyzed with GeneChip Operating Software, GCOS v1.4 using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL1261
| Sample_contact_name | Fei,,Su
| Sample_contact_institute | university of houston
| Sample_contact_address | 3605 Cullen Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77204-5056
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850394/suppl/GSM850394_wt_vp_Mouse430.2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850394/suppl/GSM850394_wt_vp_Mouse430.2.CHP.gz
| Sample_series_id | GSE34511
| Sample_data_row_count | 45101
| |
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GSM850395 | GPL1261 |
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U19/EAF2 knockout ventral prostates
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3-month-old male mouse, ventral prostate
|
tissue: ventral prostate
genotype: U19/Eaf2 -/-
genetic background: C57BL/6J
age: 3-month-old
|
Gene expression data from ventral prostate
|
Sample_geo_accession | GSM850395
| Sample_status | Public on Dec 17 2011
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Dec 17 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed following RNeasy Mini Kit QIAGEN Cat # 74104 according to the manufacturer's instructions. RNA quality and quantity were measured with spectrophotometry.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using MEGAscriptTM system according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15-20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the HP ChipScanner (Affymetrix).
| Sample_data_processing | The data were analyzed with GeneChip Operating Software, GCOS v1.4 using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL1261
| Sample_contact_name | Fei,,Su
| Sample_contact_institute | university of houston
| Sample_contact_address | 3605 Cullen Blvd
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77204-5056
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850395/suppl/GSM850395_ko_vp_Mouse430.2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850395/suppl/GSM850395_ko_vp_Mouse430.2.CHP.gz
| Sample_series_id | GSE34511
| Sample_data_row_count | 45101
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