Result

Search results for the GEO ID: GSE34514

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM850432

GPL570

Asthenozoospermic individual: Sample A1 .

Human sperm from a severe astenozoospermic infertile patient

fertility status: Infetile

c3632.CEL

Sample_geo_accession	GSM850432
Sample_status	Public on Mar 15 2012
Sample_submission_date	Dec 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Human donor Healthy human donor Healthy human donor Healthy human donor
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
Sample_hyb_protocol	GeneChip Hybridization wash and stain kit
Sample_scan_protocol	GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
Sample_data_processing	Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Sample_platform_id	GPL570
Sample_contact_name	Rafael,,Oliva
Sample_contact_email	roliva@ub.edu
Sample_contact_phone	+34934081877
Sample_contact_laboratory	Genètica Humana
Sample_contact_department	Ciències Fisiològiques I
Sample_contact_institute	University of Barcelona
Sample_contact_address	C/Casanova 143
Sample_contact_city	Barcelona
Sample_contact_zip/postal_code	08036
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850432/suppl/GSM850432.CEL.gz
Sample_series_id	GSE34514
Sample_data_row_count	54675

GSM850433

GPL570

Asthenozoospermic individual: Sample A2 .

Human sperm from a severe astenozoospermic infertile patient

fertility status: Infertile

c3643.CEL

Sample_geo_accession	GSM850433
Sample_status	Public on Mar 15 2012
Sample_submission_date	Dec 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Human donor Healthy human donor Healthy human donor Healthy human donor
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
Sample_hyb_protocol	GeneChip Hybridization wash and stain kit
Sample_scan_protocol	GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
Sample_data_processing	Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Sample_platform_id	GPL570
Sample_contact_name	Rafael,,Oliva
Sample_contact_email	roliva@ub.edu
Sample_contact_phone	+34934081877
Sample_contact_laboratory	Genètica Humana
Sample_contact_department	Ciències Fisiològiques I
Sample_contact_institute	University of Barcelona
Sample_contact_address	C/Casanova 143
Sample_contact_city	Barcelona
Sample_contact_zip/postal_code	08036
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850433/suppl/GSM850433.CEL.gz
Sample_series_id	GSE34514
Sample_data_row_count	54675

GSM850434

GPL570

Asthenozoospermic individual: Sample A3 .

Human sperm from a severe astenozoospermic infertile patient

fertility status: Infertile

c3953.CEL

Sample_geo_accession	GSM850434
Sample_status	Public on Mar 15 2012
Sample_submission_date	Dec 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Human donor Healthy human donor Healthy human donor Healthy human donor
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
Sample_hyb_protocol	GeneChip Hybridization wash and stain kit
Sample_scan_protocol	GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
Sample_data_processing	Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Sample_platform_id	GPL570
Sample_contact_name	Rafael,,Oliva
Sample_contact_email	roliva@ub.edu
Sample_contact_phone	+34934081877
Sample_contact_laboratory	Genètica Humana
Sample_contact_department	Ciències Fisiològiques I
Sample_contact_institute	University of Barcelona
Sample_contact_address	C/Casanova 143
Sample_contact_city	Barcelona
Sample_contact_zip/postal_code	08036
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850434/suppl/GSM850434.CEL.gz
Sample_series_id	GSE34514
Sample_data_row_count	54675

GSM850435

GPL570

Asthenozoospermic individual: Sample A4 .

Human sperm from a severe astenozoospermic infertile patient

fertility status: Infertile

c4125.CEL

Sample_geo_accession	GSM850435
Sample_status	Public on Mar 15 2012
Sample_submission_date	Dec 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Human donor Healthy human donor Healthy human donor Healthy human donor
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
Sample_hyb_protocol	GeneChip Hybridization wash and stain kit
Sample_scan_protocol	GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
Sample_data_processing	Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Sample_platform_id	GPL570
Sample_contact_name	Rafael,,Oliva
Sample_contact_email	roliva@ub.edu
Sample_contact_phone	+34934081877
Sample_contact_laboratory	Genètica Humana
Sample_contact_department	Ciències Fisiològiques I
Sample_contact_institute	University of Barcelona
Sample_contact_address	C/Casanova 143
Sample_contact_city	Barcelona
Sample_contact_zip/postal_code	08036
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850435/suppl/GSM850435.CEL.gz
Sample_series_id	GSE34514
Sample_data_row_count	54675

GSM850436

GPL570

Normozoospermic individual: Sample N1

Human sperm from a normozoospermic fertile control

fertility status: Fertile

D221.CEL

Sample_geo_accession	GSM850436
Sample_status	Public on Mar 15 2012
Sample_submission_date	Dec 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Human donor Healthy human donor Healthy human donor Healthy human donor
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
Sample_hyb_protocol	GeneChip Hybridization wash and stain kit
Sample_scan_protocol	GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
Sample_data_processing	Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Sample_platform_id	GPL570
Sample_contact_name	Rafael,,Oliva
Sample_contact_email	roliva@ub.edu
Sample_contact_phone	+34934081877
Sample_contact_laboratory	Genètica Humana
Sample_contact_department	Ciències Fisiològiques I
Sample_contact_institute	University of Barcelona
Sample_contact_address	C/Casanova 143
Sample_contact_city	Barcelona
Sample_contact_zip/postal_code	08036
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850436/suppl/GSM850436.CEL.gz
Sample_series_id	GSE34514
Sample_data_row_count	54675

GSM850437

GPL570

Normozoospermic individual: Sample N2

Human sperm from a normozoospermic fertile control

fertility status: Fertile

D257.CEL

Sample_geo_accession	GSM850437
Sample_status	Public on Mar 15 2012
Sample_submission_date	Dec 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Human donor Healthy human donor Healthy human donor Healthy human donor
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
Sample_hyb_protocol	GeneChip Hybridization wash and stain kit
Sample_scan_protocol	GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
Sample_data_processing	Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Sample_platform_id	GPL570
Sample_contact_name	Rafael,,Oliva
Sample_contact_email	roliva@ub.edu
Sample_contact_phone	+34934081877
Sample_contact_laboratory	Genètica Humana
Sample_contact_department	Ciències Fisiològiques I
Sample_contact_institute	University of Barcelona
Sample_contact_address	C/Casanova 143
Sample_contact_city	Barcelona
Sample_contact_zip/postal_code	08036
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850437/suppl/GSM850437.CEL.gz
Sample_series_id	GSE34514
Sample_data_row_count	54675

GSM850438

GPL570

Normozoospermic individual: Sample N3

Human sperm from a normozoospermic fertile control

fertility status: Fertile

D258.CEL

Sample_geo_accession	GSM850438
Sample_status	Public on Mar 15 2012
Sample_submission_date	Dec 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Human donor Healthy human donor Healthy human donor Healthy human donor
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
Sample_hyb_protocol	GeneChip Hybridization wash and stain kit
Sample_scan_protocol	GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
Sample_data_processing	Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Sample_platform_id	GPL570
Sample_contact_name	Rafael,,Oliva
Sample_contact_email	roliva@ub.edu
Sample_contact_phone	+34934081877
Sample_contact_laboratory	Genètica Humana
Sample_contact_department	Ciències Fisiològiques I
Sample_contact_institute	University of Barcelona
Sample_contact_address	C/Casanova 143
Sample_contact_city	Barcelona
Sample_contact_zip/postal_code	08036
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850438/suppl/GSM850438.CEL.gz
Sample_series_id	GSE34514
Sample_data_row_count	54675

GSM850439

GPL570

Normozoospermic individual: Sample N4

Human sperm from a normozoospermic fertile control

fertility status: Fertile

D278.CEL

Sample_geo_accession	GSM850439
Sample_status	Public on Mar 15 2012
Sample_submission_date	Dec 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	Human donor Healthy human donor Healthy human donor Healthy human donor
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
Sample_hyb_protocol	GeneChip Hybridization wash and stain kit
Sample_scan_protocol	GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
Sample_data_processing	Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
Sample_platform_id	GPL570
Sample_contact_name	Rafael,,Oliva
Sample_contact_email	roliva@ub.edu
Sample_contact_phone	+34934081877
Sample_contact_laboratory	Genètica Humana
Sample_contact_department	Ciències Fisiològiques I
Sample_contact_institute	University of Barcelona
Sample_contact_address	C/Casanova 143
Sample_contact_city	Barcelona
Sample_contact_zip/postal_code	08036
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850439/suppl/GSM850439.CEL.gz
Sample_series_id	GSE34514
Sample_data_row_count	54675

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