Search results for the GEO ID: GSE34514 ![](/q11.jpeg) |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM850432 | GPL570 |
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Asthenozoospermic individual: Sample A1 .
|
Human sperm from a severe astenozoospermic infertile patient
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fertility status: Infetile
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c3632.CEL
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Sample_geo_accession | GSM850432
| Sample_status | Public on Mar 15 2012
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Mar 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human donor Healthy human donor Healthy human donor Healthy human donor
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| Sample_platform_id | GPL570
| Sample_contact_name | Rafael,,Oliva
| Sample_contact_email | roliva@ub.edu
| Sample_contact_phone | +34934081877
| Sample_contact_laboratory | Genètica Humana
| Sample_contact_department | Ciències Fisiològiques I
| Sample_contact_institute | University of Barcelona
| Sample_contact_address | C/Casanova 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850432/suppl/GSM850432.CEL.gz
| Sample_series_id | GSE34514
| Sample_data_row_count | 54675
| |
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GSM850433 | GPL570 |
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Asthenozoospermic individual: Sample A2 .
|
Human sperm from a severe astenozoospermic infertile patient
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fertility status: Infertile
|
c3643.CEL
|
Sample_geo_accession | GSM850433
| Sample_status | Public on Mar 15 2012
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Mar 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human donor Healthy human donor Healthy human donor Healthy human donor
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| Sample_platform_id | GPL570
| Sample_contact_name | Rafael,,Oliva
| Sample_contact_email | roliva@ub.edu
| Sample_contact_phone | +34934081877
| Sample_contact_laboratory | Genètica Humana
| Sample_contact_department | Ciències Fisiològiques I
| Sample_contact_institute | University of Barcelona
| Sample_contact_address | C/Casanova 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850433/suppl/GSM850433.CEL.gz
| Sample_series_id | GSE34514
| Sample_data_row_count | 54675
| |
|
GSM850434 | GPL570 |
|
Asthenozoospermic individual: Sample A3 .
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Human sperm from a severe astenozoospermic infertile patient
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fertility status: Infertile
|
c3953.CEL
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Sample_geo_accession | GSM850434
| Sample_status | Public on Mar 15 2012
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Mar 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human donor Healthy human donor Healthy human donor Healthy human donor
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| Sample_platform_id | GPL570
| Sample_contact_name | Rafael,,Oliva
| Sample_contact_email | roliva@ub.edu
| Sample_contact_phone | +34934081877
| Sample_contact_laboratory | Genètica Humana
| Sample_contact_department | Ciències Fisiològiques I
| Sample_contact_institute | University of Barcelona
| Sample_contact_address | C/Casanova 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850434/suppl/GSM850434.CEL.gz
| Sample_series_id | GSE34514
| Sample_data_row_count | 54675
| |
|
GSM850435 | GPL570 |
|
Asthenozoospermic individual: Sample A4 .
|
Human sperm from a severe astenozoospermic infertile patient
|
fertility status: Infertile
|
c4125.CEL
|
Sample_geo_accession | GSM850435
| Sample_status | Public on Mar 15 2012
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Mar 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human donor Healthy human donor Healthy human donor Healthy human donor
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| Sample_platform_id | GPL570
| Sample_contact_name | Rafael,,Oliva
| Sample_contact_email | roliva@ub.edu
| Sample_contact_phone | +34934081877
| Sample_contact_laboratory | Genètica Humana
| Sample_contact_department | Ciències Fisiològiques I
| Sample_contact_institute | University of Barcelona
| Sample_contact_address | C/Casanova 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850435/suppl/GSM850435.CEL.gz
| Sample_series_id | GSE34514
| Sample_data_row_count | 54675
| |
|
GSM850436 | GPL570 |
|
Normozoospermic individual: Sample N1
|
Human sperm from a normozoospermic fertile control
|
fertility status: Fertile
|
D221.CEL
|
Sample_geo_accession | GSM850436
| Sample_status | Public on Mar 15 2012
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Mar 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human donor Healthy human donor Healthy human donor Healthy human donor
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| Sample_platform_id | GPL570
| Sample_contact_name | Rafael,,Oliva
| Sample_contact_email | roliva@ub.edu
| Sample_contact_phone | +34934081877
| Sample_contact_laboratory | Genètica Humana
| Sample_contact_department | Ciències Fisiològiques I
| Sample_contact_institute | University of Barcelona
| Sample_contact_address | C/Casanova 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850436/suppl/GSM850436.CEL.gz
| Sample_series_id | GSE34514
| Sample_data_row_count | 54675
| |
|
GSM850437 | GPL570 |
|
Normozoospermic individual: Sample N2
|
Human sperm from a normozoospermic fertile control
|
fertility status: Fertile
|
D257.CEL
|
Sample_geo_accession | GSM850437
| Sample_status | Public on Mar 15 2012
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Mar 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human donor Healthy human donor Healthy human donor Healthy human donor
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| Sample_platform_id | GPL570
| Sample_contact_name | Rafael,,Oliva
| Sample_contact_email | roliva@ub.edu
| Sample_contact_phone | +34934081877
| Sample_contact_laboratory | Genètica Humana
| Sample_contact_department | Ciències Fisiològiques I
| Sample_contact_institute | University of Barcelona
| Sample_contact_address | C/Casanova 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850437/suppl/GSM850437.CEL.gz
| Sample_series_id | GSE34514
| Sample_data_row_count | 54675
| |
|
GSM850438 | GPL570 |
|
Normozoospermic individual: Sample N3
|
Human sperm from a normozoospermic fertile control
|
fertility status: Fertile
|
D258.CEL
|
Sample_geo_accession | GSM850438
| Sample_status | Public on Mar 15 2012
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Mar 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human donor Healthy human donor Healthy human donor Healthy human donor
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| Sample_platform_id | GPL570
| Sample_contact_name | Rafael,,Oliva
| Sample_contact_email | roliva@ub.edu
| Sample_contact_phone | +34934081877
| Sample_contact_laboratory | Genètica Humana
| Sample_contact_department | Ciències Fisiològiques I
| Sample_contact_institute | University of Barcelona
| Sample_contact_address | C/Casanova 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850438/suppl/GSM850438.CEL.gz
| Sample_series_id | GSE34514
| Sample_data_row_count | 54675
| |
|
GSM850439 | GPL570 |
|
Normozoospermic individual: Sample N4
|
Human sperm from a normozoospermic fertile control
|
fertility status: Fertile
|
D278.CEL
|
Sample_geo_accession | GSM850439
| Sample_status | Public on Mar 15 2012
| Sample_submission_date | Dec 16 2011
| Sample_last_update_date | Mar 15 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human donor Healthy human donor Healthy human donor Healthy human donor
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Semen was collected and to rule out the possibility of any somatic cells contamination, we performed a 50% Percoll Gradient followed by PBS washes. After this initial purification, the samples are treated with Somatic Cell Lysis Buffer (0.1% SDS, 0.5% TX-100). Microscopic examination was then used to verify the elimination of the somatic cells. The RNA was then extracted from 15- 30 x106 sperm cells and purified using the Qiagen miRNeasy kit according to the manufacture’s recommendations. The RNA samples were subjected to different quality controls.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
| Sample_hyb_protocol | GeneChip Hybridization wash and stain kit
| Sample_scan_protocol | GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)
| Sample_data_processing | Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.
| Sample_platform_id | GPL570
| Sample_contact_name | Rafael,,Oliva
| Sample_contact_email | roliva@ub.edu
| Sample_contact_phone | +34934081877
| Sample_contact_laboratory | Genètica Humana
| Sample_contact_department | Ciències Fisiològiques I
| Sample_contact_institute | University of Barcelona
| Sample_contact_address | C/Casanova 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850439/suppl/GSM850439.CEL.gz
| Sample_series_id | GSE34514
| Sample_data_row_count | 54675
| |
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