Search results for the GEO ID: GSE34537  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM850705 | GPL1261 |
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Flk1+Tie2+ cells, A2lox.Mesp1 day 5 EBs, untreated
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Flk1+Tie2+ cells purified from A2lox.Mesp1 day 5 embryoid bodies without doxycycline
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cell type: dox-inducible transgenic A2lox.Mesp1 mouse embryonic stem cells
background strain: 129/Ola
day of differentiation: 5
treatment: untreated
population: Flk1+Tie2+
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Mesp1-dox_Flk1+Tie2+
Gene expression data from Flk1+Tie2+ cells purified from A2lox.Mesp1 day 5 embryoid bodies without doxycycline.
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| Sample_geo_accession | GSM850705
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Dec 19 2011
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In dox-treated conditions, doxycycline was added at a final concentration of 250ng/mL on day 2. On day 5, EBs were trypsinized and stained with antibodies against Flk1 and Tie2. Flk1+Tie2+ endothelial cells were sorted using a MoFlo cytometer.
| | Sample_growth_protocol_ch1 | A2lox.Mesp1 ES cells were differentiated as embryoid bodies in petri dishes at a density of 15,000 cells/mL in differentiation media consisting of 10% FCS for 5 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA from sorted cell populations was extracted using Qiagen's RNeasy kits according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the Affymetrix GeneChip 3' IVT Express Kit protocol from 100 ng total RNA (GeneChip 3' IVT Express Kit manual, 2008-2009, Affymetrix).
| | Sample_hyb_protocol | The Affymetrix operating system (Command Console) was used to manage the washing and hybridization steps.
| | Sample_scan_protocol | The Affymetrix operating system (Command Console) was used for scanning. Affymetrix analysis software (Genotyping Console) was used to derive Quality Control metrics, create .CEL files and scaled expression data from the scanned expression array image files.
| | Sample_data_processing | The data was analyzed with dChip 2010 using default analysis settings. Both samples were normalized to a single baseline array of median intensity.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mi,,Cai
| | Sample_contact_email | mcai@wustl.edu
| | Sample_contact_phone | 314-362-2004
| | Sample_contact_fax | 314-747-4888
| | Sample_contact_laboratory | Ken Murphy
| | Sample_contact_department | Immunology and Pathology
| | Sample_contact_institute | Washington University School of Medicine
| | Sample_contact_address | 660 S. Euclid Ave.
| | Sample_contact_city | St. Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850705/suppl/GSM850705.CEL.gz
| | Sample_series_id | GSE34537
| | Sample_series_id | GSE34583
| | Sample_data_row_count | 45037
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GSM850706 | GPL1261 |
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Flk1+Tie2+ cells, A2lox.Mesp1 day 5 EBs, treated with dox
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Flk1+Tie2+ cells purified from A2lox.Mesp1 day 5 embryoid bodies treated with doxycyline from day 2 to day 4
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cell type: dox-inducible transgenic A2lox.Mesp1 mouse embryonic stem cells
background strain: 129/Ola
day of differentiation: 5
treatment: doxycycline on day 2
population: Flk1+Tie2+
|
Mesp1+dox_Flk1+Tie2+
Gene expression data from Flk1+Tie2+ cells purified from A2lox.Mesp1 day 5 embryoid bodies treated with doxycycline from day 2 to day 4.
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| Sample_geo_accession | GSM850706
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Dec 19 2011
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In dox-treated conditions, doxycycline was added at a final concentration of 250ng/mL on day 2. On day 5, EBs were trypsinized and stained with antibodies against Flk1 and Tie2. Flk1+Tie2+ endothelial cells were sorted using a MoFlo cytometer.
| | Sample_growth_protocol_ch1 | A2lox.Mesp1 ES cells were differentiated as embryoid bodies in petri dishes at a density of 15,000 cells/mL in differentiation media consisting of 10% FCS for 5 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA from sorted cell populations was extracted using Qiagen's RNeasy kits according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the Affymetrix GeneChip 3' IVT Express Kit protocol from 100 ng total RNA (GeneChip 3' IVT Express Kit manual, 2008-2009, Affymetrix).
| | Sample_hyb_protocol | The Affymetrix operating system (Command Console) was used to manage the washing and hybridization steps.
| | Sample_scan_protocol | The Affymetrix operating system (Command Console) was used for scanning. Affymetrix analysis software (Genotyping Console) was used to derive Quality Control metrics, create .CEL files and scaled expression data from the scanned expression array image files.
| | Sample_data_processing | The data was analyzed with dChip 2010 using default analysis settings. Both samples were normalized to a single baseline array of median intensity.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mi,,Cai
| | Sample_contact_email | mcai@wustl.edu
| | Sample_contact_phone | 314-362-2004
| | Sample_contact_fax | 314-747-4888
| | Sample_contact_laboratory | Ken Murphy
| | Sample_contact_department | Immunology and Pathology
| | Sample_contact_institute | Washington University School of Medicine
| | Sample_contact_address | 660 S. Euclid Ave.
| | Sample_contact_city | St. Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM850nnn/GSM850706/suppl/GSM850706.CEL.gz
| | Sample_series_id | GSE34537
| | Sample_series_id | GSE34583
| | Sample_data_row_count | 45037
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