Result

Search results for the GEO ID: GSE34586

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM851629

GPL339

Control keratinocytes treated with EtOH

Prdm1 floxed mice without CreER expression, EtOH

congenic strain: C57BL/6 tissue: tail skin cell type: primary cultured keratinocytes treatment: ethanol (EtOH)

control EtOH Tail skin keratinocytes from control mice were cultured in serum-free keratinocytes conditional medium with solvent control ethanol (EtOH).

Sample_geo_accession	GSM851629
Sample_status	Public on Apr 02 2013
Sample_submission_date	Dec 20 2011
Sample_last_update_date	Apr 03 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	4-hydroxytamoxifen (4OHT) or ethanol (EtOH, solvent control) were added to culture medium from day 1 to day 3.
Sample_growth_protocol_ch1	Tail skin keratinocytes were cultured in serum-free medium, CnT-07 (CELLnTEC), and rat collagen I coated plates. Culture medium was refreshed on day 3 and cells were collected on day 5.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagn RNeasy® Mini kit, followed by using the Qiagen RNase-Free DNase Set to remove DNA. The isolation procedure was performed per the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Microarray was performed according to the protocol provided by the manufacturer (3' IVT express kit, Affymetrix). Total RNAs were used as the template to generate double-strand cDNAs. Total RNAs were degraded and double-strand cDNAs were purified by cDNA Cleanup Spin Column (Qiagen). cRNAs with biotin-conjugated nucleotide analog were generated and amplified by in vitro transcription using T7 RNA polymerase.
Sample_hyb_protocol	The biotinylated cRNAs were hybridized to GeneChip® Mouse Expression Array 430A.
Sample_scan_protocol	Microarray was scanned and images were acquired by GeneChip® Scanner 3000.
Sample_data_processing	Data were processed by the RMA algorithm using GeneSpring GX11.
Sample_platform_id	GPL339
Sample_contact_name	Ming-Feng,,Chiang
Sample_contact_department	Genomics Research Center
Sample_contact_institute	Academia sinica
Sample_contact_address	128 Academia Road, Section 2
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	115
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851629/suppl/GSM851629.CEL.gz
Sample_series_id	GSE34586
Sample_data_row_count	22626

GSM851630

GPL339

Control keratinocytes treated with 4OHT

Prdm1 floxed mice without CreER expression, 4OHT

congenic strain: C57BL/6 tissue: tail skin cell type: primary cultured keratinocytes treatment: 4-hydroxytamoxifen (4OHT)

control 4OHT Tail skin keratinocytes from control mice were cultured in serum-free keratinocytes conditional medium with 4-hydroxytamoxifen (4OHT).

Sample_geo_accession	GSM851630
Sample_status	Public on Apr 02 2013
Sample_submission_date	Dec 20 2011
Sample_last_update_date	Apr 03 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	4-hydroxytamoxifen (4OHT) or ethanol (EtOH, solvent control) were added to culture medium from day 1 to day 3.
Sample_growth_protocol_ch1	Tail skin keratinocytes were cultured in serum-free medium, CnT-07 (CELLnTEC), and rat collagen I coated plates. Culture medium was refreshed on day 3 and cells were collected on day 5.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagn RNeasy® Mini kit, followed by using the Qiagen RNase-Free DNase Set to remove DNA. The isolation procedure was performed per the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Microarray was performed according to the protocol provided by the manufacturer (3' IVT express kit, Affymetrix). Total RNAs were used as the template to generate double-strand cDNAs. Total RNAs were degraded and double-strand cDNAs were purified by cDNA Cleanup Spin Column (Qiagen). cRNAs with biotin-conjugated nucleotide analog were generated and amplified by in vitro transcription using T7 RNA polymerase.
Sample_hyb_protocol	The biotinylated cRNAs were hybridized to GeneChip® Mouse Expression Array 430A.
Sample_scan_protocol	Microarray was scanned and images were acquired by GeneChip® Scanner 3000.
Sample_data_processing	Data were processed by the RMA algorithm using GeneSpring GX11.
Sample_platform_id	GPL339
Sample_contact_name	Ming-Feng,,Chiang
Sample_contact_department	Genomics Research Center
Sample_contact_institute	Academia sinica
Sample_contact_address	128 Academia Road, Section 2
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	115
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851630/suppl/GSM851630.CEL.gz
Sample_series_id	GSE34586
Sample_data_row_count	22626

GSM851631

GPL339

CKO keratinocytes treated with EtOH

Prdm1 floxed mice with CreER expression, EtOH

congenic strain: C57BL/6 tissue: tail skin cell type: primary cultured keratinocytes treatment: ethanol (EtOH)

CKO EtOH Tail skin keratinocytes from CKO mice were cultured in serum-free keratinocytes conditional medium with solvent control ethanol (EtOH).

Sample_geo_accession	GSM851631
Sample_status	Public on Apr 02 2013
Sample_submission_date	Dec 20 2011
Sample_last_update_date	Apr 03 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	4-hydroxytamoxifen (4OHT) or ethanol (EtOH, solvent control) were added to culture medium from day 1 to day 3.
Sample_growth_protocol_ch1	Tail skin keratinocytes were cultured in serum-free medium, CnT-07 (CELLnTEC), and rat collagen I coated plates. Culture medium was refreshed on day 3 and cells were collected on day 5.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagn RNeasy® Mini kit, followed by using the Qiagen RNase-Free DNase Set to remove DNA. The isolation procedure was performed per the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Microarray was performed according to the protocol provided by the manufacturer (3' IVT express kit, Affymetrix). Total RNAs were used as the template to generate double-strand cDNAs. Total RNAs were degraded and double-strand cDNAs were purified by cDNA Cleanup Spin Column (Qiagen). cRNAs with biotin-conjugated nucleotide analog were generated and amplified by in vitro transcription using T7 RNA polymerase.
Sample_hyb_protocol	The biotinylated cRNAs were hybridized to GeneChip® Mouse Expression Array 430A.
Sample_scan_protocol	Microarray was scanned and images were acquired by GeneChip® Scanner 3000.
Sample_data_processing	Data were processed by the RMA algorithm using GeneSpring GX11.
Sample_platform_id	GPL339
Sample_contact_name	Ming-Feng,,Chiang
Sample_contact_department	Genomics Research Center
Sample_contact_institute	Academia sinica
Sample_contact_address	128 Academia Road, Section 2
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	115
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851631/suppl/GSM851631.CEL.gz
Sample_series_id	GSE34586
Sample_data_row_count	22626

GSM851632

GPL339

CKO keratinocytes treated with 4OHT

Prdm1 floxed mice with CreER expression, 4OHT

congenic strain: C57BL/6 tissue: tail skin cell type: primary cultured keratinocytes treatment: 4-hydroxytamoxifen (4OHT)

CKO 4OHT Tail skin keratinocytes from CKO mice were cultured in serum-free keratinocytes conditional medium with 4-hydroxytamoxifen (4OHT).

Sample_geo_accession	GSM851632
Sample_status	Public on Apr 02 2013
Sample_submission_date	Dec 20 2011
Sample_last_update_date	Apr 03 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	4-hydroxytamoxifen (4OHT) or ethanol (EtOH, solvent control) were added to culture medium from day 1 to day 3.
Sample_growth_protocol_ch1	Tail skin keratinocytes were cultured in serum-free medium, CnT-07 (CELLnTEC), and rat collagen I coated plates. Culture medium was refreshed on day 3 and cells were collected on day 5.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the Qiagn RNeasy® Mini kit, followed by using the Qiagen RNase-Free DNase Set to remove DNA. The isolation procedure was performed per the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Microarray was performed according to the protocol provided by the manufacturer (3' IVT express kit, Affymetrix). Total RNAs were used as the template to generate double-strand cDNAs. Total RNAs were degraded and double-strand cDNAs were purified by cDNA Cleanup Spin Column (Qiagen). cRNAs with biotin-conjugated nucleotide analog were generated and amplified by in vitro transcription using T7 RNA polymerase.
Sample_hyb_protocol	The biotinylated cRNAs were hybridized to GeneChip® Mouse Expression Array 430A.
Sample_scan_protocol	Microarray was scanned and images were acquired by GeneChip® Scanner 3000.
Sample_data_processing	Data were processed by the RMA algorithm using GeneSpring GX11.
Sample_platform_id	GPL339
Sample_contact_name	Ming-Feng,,Chiang
Sample_contact_department	Genomics Research Center
Sample_contact_institute	Academia sinica
Sample_contact_address	128 Academia Road, Section 2
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	115
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851632/suppl/GSM851632.CEL.gz
Sample_series_id	GSE34586
Sample_data_row_count	22626

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