Search results for the GEO ID: GSE34618  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM851976 | GPL1261 |
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CD8+ Central memory T cells before treatment with Con A, biological rep1
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CD8+ Central memory T cells before activation
|
source tissue: Spleen
cell type: CD8+ Central memory T
treatment: untreated
time: 0 h
gender: male
age: 62 wk
strain: C57BL/6
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Gene expression data from CD8+ Central memory T cells before activation
|
| Sample_geo_accession | GSM851976
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Dec 21 2011
| | Sample_last_update_date | May 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected and cultured (0.5 x106 cells/well) in 10% complete RPMI media supplemented with 0.005 ugr Con A.
| | Sample_growth_protocol_ch1 | Ten spleens were pooled from 62 week old C57BL/6 male mice and enriched for CD8+ T cells by negative selection using a cocktail of biotinylated antibodies to deplete CD4, CD11c, CD11b, MHC-II, B220, and NK cells. Cells were then stained for CD8, CD44, CD62L, and CD127 and sorted (using a iCyt Reflection parallel cell sorter).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol Reagent (Invitrogen) followed by RNeasy clean-up kit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples with a RNA Integrity Number (RIN) >7.0 were considered suitable for labeling and 20 ng were labeled using the GeneChip two-cycle target labeling kit (Affymetrix).
| | Sample_hyb_protocol | 0.01 mg of labeled and fragmented cRNA were hybridized to the mouse genome MOE430 2.0 array (Affymetrix).
| | Sample_scan_protocol | Raw expression data were analyzed using GCOS 1.4 (Affymetrix). Data were normalized to a target intensity of 500 to account for differences in global chip intensity.
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Roy,,Blum
| | Sample_contact_email | blumroy@gmail.com
| | Sample_contact_phone | 212-263-8129
| | Sample_contact_fax | 212-263-8139
| | Sample_contact_laboratory | Alan Frey Lab
| | Sample_contact_department | Cell Biology
| | Sample_contact_institute | NYU Medical Center
| | Sample_contact_address | 550 First Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.med.nyu.edu/biosketch/freya01/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851976/suppl/GSM851976_Cm_T0.CEL.gz
| | Sample_series_id | GSE34618
| | Sample_data_row_count | 45101
| | |
|
GSM851977 | GPL1261 |
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ConA-activated CD8+ Central memory T cells at T4, biological rep1
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CD8+ Central memory T cells after activation with 0.005 ugr Con A
|
source tissue: Spleen
cell type: CD8+ Central memory T
treatment: ConA
time: 4 h
gender: male
age: 62 wk
strain: C57BL/6
|
Gene expression data from CD8+ Central memory T cells after activation with Con A
|
| Sample_geo_accession | GSM851977
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Dec 21 2011
| | Sample_last_update_date | May 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected and cultured (0.5 x106 cells/well) in 10% complete RPMI media supplemented with 0.005 ugr Con A.
| | Sample_growth_protocol_ch1 | Ten spleens were pooled from 62 week old C57BL/6 male mice and enriched for CD8+ T cells by negative selection using a cocktail of biotinylated antibodies to deplete CD4, CD11c, CD11b, MHC-II, B220, and NK cells. Cells were then stained for CD8, CD44, CD62L, and CD127 and sorted (using a iCyt Reflection parallel cell sorter).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol Reagent (Invitrogen) followed by RNeasy clean-up kit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples with a RNA Integrity Number (RIN) >7.0 were considered suitable for labeling and 20 ng were labeled using the GeneChip two-cycle target labeling kit (Affymetrix).
| | Sample_hyb_protocol | 0.01 mg of labeled and fragmented cRNA were hybridized to the mouse genome MOE430 2.0 array (Affymetrix).
| | Sample_scan_protocol | Raw expression data were analyzed using GCOS 1.4 (Affymetrix). Data were normalized to a target intensity of 500 to account for differences in global chip intensity.
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Roy,,Blum
| | Sample_contact_email | blumroy@gmail.com
| | Sample_contact_phone | 212-263-8129
| | Sample_contact_fax | 212-263-8139
| | Sample_contact_laboratory | Alan Frey Lab
| | Sample_contact_department | Cell Biology
| | Sample_contact_institute | NYU Medical Center
| | Sample_contact_address | 550 First Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.med.nyu.edu/biosketch/freya01/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851977/suppl/GSM851977_Cm_T4.CEL.gz
| | Sample_series_id | GSE34618
| | Sample_data_row_count | 45101
| | |
|
GSM851978 | GPL1261 |
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ConA-activated CD8+ Central memory T cells at T6, biological rep1
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CD8+ Central memory T cells after activation with 0.005 ugr Con A
|
source tissue: Spleen
cell type: CD8+ Central memory T
treatment: ConA
time: 6 h
gender: male
age: 62 wk
strain: C57BL/6
|
Gene expression data from CD8+ Central memory T cells after activation with Con A
|
| Sample_geo_accession | GSM851978
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Dec 21 2011
| | Sample_last_update_date | May 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected and cultured (0.5 x106 cells/well) in 10% complete RPMI media supplemented with 0.005 ugr Con A.
| | Sample_growth_protocol_ch1 | Ten spleens were pooled from 62 week old C57BL/6 male mice and enriched for CD8+ T cells by negative selection using a cocktail of biotinylated antibodies to deplete CD4, CD11c, CD11b, MHC-II, B220, and NK cells. Cells were then stained for CD8, CD44, CD62L, and CD127 and sorted (using a iCyt Reflection parallel cell sorter).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol Reagent (Invitrogen) followed by RNeasy clean-up kit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples with a RNA Integrity Number (RIN) >7.0 were considered suitable for labeling and 20 ng were labeled using the GeneChip two-cycle target labeling kit (Affymetrix).
| | Sample_hyb_protocol | 0.01 mg of labeled and fragmented cRNA were hybridized to the mouse genome MOE430 2.0 array (Affymetrix).
| | Sample_scan_protocol | Raw expression data were analyzed using GCOS 1.4 (Affymetrix). Data were normalized to a target intensity of 500 to account for differences in global chip intensity.
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Roy,,Blum
| | Sample_contact_email | blumroy@gmail.com
| | Sample_contact_phone | 212-263-8129
| | Sample_contact_fax | 212-263-8139
| | Sample_contact_laboratory | Alan Frey Lab
| | Sample_contact_department | Cell Biology
| | Sample_contact_institute | NYU Medical Center
| | Sample_contact_address | 550 First Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.med.nyu.edu/biosketch/freya01/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851978/suppl/GSM851978_Cm_T6.CEL.gz
| | Sample_series_id | GSE34618
| | Sample_data_row_count | 45101
| | |
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GSM851979 | GPL1261 |
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ConA-activated CD8+ Central memory T cells at T8, biological rep1
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CD8+ Central memory T cells after activation with 0.005 ugr Con A
|
source tissue: Spleen
cell type: CD8+ Central memory T
treatment: ConA
time: 8 h
gender: male
age: 62 wk
strain: C57BL/6
|
Gene expression data from CD8+ Central memory T cells after activation with Con A
|
| Sample_geo_accession | GSM851979
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Dec 21 2011
| | Sample_last_update_date | May 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected and cultured (0.5 x106 cells/well) in 10% complete RPMI media supplemented with 0.005 ugr Con A.
| | Sample_growth_protocol_ch1 | Ten spleens were pooled from 62 week old C57BL/6 male mice and enriched for CD8+ T cells by negative selection using a cocktail of biotinylated antibodies to deplete CD4, CD11c, CD11b, MHC-II, B220, and NK cells. Cells were then stained for CD8, CD44, CD62L, and CD127 and sorted (using a iCyt Reflection parallel cell sorter).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol Reagent (Invitrogen) followed by RNeasy clean-up kit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples with a RNA Integrity Number (RIN) >7.0 were considered suitable for labeling and 20 ng were labeled using the GeneChip two-cycle target labeling kit (Affymetrix).
| | Sample_hyb_protocol | 0.01 mg of labeled and fragmented cRNA were hybridized to the mouse genome MOE430 2.0 array (Affymetrix).
| | Sample_scan_protocol | Raw expression data were analyzed using GCOS 1.4 (Affymetrix). Data were normalized to a target intensity of 500 to account for differences in global chip intensity.
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Roy,,Blum
| | Sample_contact_email | blumroy@gmail.com
| | Sample_contact_phone | 212-263-8129
| | Sample_contact_fax | 212-263-8139
| | Sample_contact_laboratory | Alan Frey Lab
| | Sample_contact_department | Cell Biology
| | Sample_contact_institute | NYU Medical Center
| | Sample_contact_address | 550 First Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.med.nyu.edu/biosketch/freya01/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851979/suppl/GSM851979_Cm_T8.CEL.gz
| | Sample_series_id | GSE34618
| | Sample_data_row_count | 45101
| | |
|
GSM851980 | GPL1261 |
|
ConA-activated CD8+ Central memory T cells at T16, biological rep1
|
CD8+ Central memory T cells after activation with 0.005 ugr Con A
|
source tissue: Spleen
cell type: CD8+ Central memory T
treatment: ConA
time: 16 h
gender: male
age: 62 wk
strain: C57BL/6
|
Gene expression data from CD8+ Central memory T cells after activation with Con A
|
| Sample_geo_accession | GSM851980
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Dec 21 2011
| | Sample_last_update_date | May 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected and cultured (0.5 x106 cells/well) in 10% complete RPMI media supplemented with 0.005 ugr Con A.
| | Sample_growth_protocol_ch1 | Ten spleens were pooled from 62 week old C57BL/6 male mice and enriched for CD8+ T cells by negative selection using a cocktail of biotinylated antibodies to deplete CD4, CD11c, CD11b, MHC-II, B220, and NK cells. Cells were then stained for CD8, CD44, CD62L, and CD127 and sorted (using a iCyt Reflection parallel cell sorter).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol Reagent (Invitrogen) followed by RNeasy clean-up kit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples with a RNA Integrity Number (RIN) >7.0 were considered suitable for labeling and 20 ng were labeled using the GeneChip two-cycle target labeling kit (Affymetrix).
| | Sample_hyb_protocol | 0.01 mg of labeled and fragmented cRNA were hybridized to the mouse genome MOE430 2.0 array (Affymetrix).
| | Sample_scan_protocol | Raw expression data were analyzed using GCOS 1.4 (Affymetrix). Data were normalized to a target intensity of 500 to account for differences in global chip intensity.
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Roy,,Blum
| | Sample_contact_email | blumroy@gmail.com
| | Sample_contact_phone | 212-263-8129
| | Sample_contact_fax | 212-263-8139
| | Sample_contact_laboratory | Alan Frey Lab
| | Sample_contact_department | Cell Biology
| | Sample_contact_institute | NYU Medical Center
| | Sample_contact_address | 550 First Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.med.nyu.edu/biosketch/freya01/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851980/suppl/GSM851980_Cm_T16.CEL.gz
| | Sample_series_id | GSE34618
| | Sample_data_row_count | 45101
| | |
|
GSM851981 | GPL1261 |
|
ConA-activated CD8+ Central memory T cells at T20, biological rep1
|
CD8+ Central memory T cells after activation with 0.005 ugr Con A
|
source tissue: Spleen
cell type: CD8+ Central memory T
treatment: ConA
time: 20 h
gender: male
age: 62 wk
strain: C57BL/6
|
Gene expression data from CD8+ Central memory T cells after activation with Con A
|
| Sample_geo_accession | GSM851981
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Dec 21 2011
| | Sample_last_update_date | May 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected and cultured (0.5 x106 cells/well) in 10% complete RPMI media supplemented with 0.005 ugr Con A.
| | Sample_growth_protocol_ch1 | Ten spleens were pooled from 62 week old C57BL/6 male mice and enriched for CD8+ T cells by negative selection using a cocktail of biotinylated antibodies to deplete CD4, CD11c, CD11b, MHC-II, B220, and NK cells. Cells were then stained for CD8, CD44, CD62L, and CD127 and sorted (using a iCyt Reflection parallel cell sorter).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol Reagent (Invitrogen) followed by RNeasy clean-up kit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples with a RNA Integrity Number (RIN) >7.0 were considered suitable for labeling and 20 ng were labeled using the GeneChip two-cycle target labeling kit (Affymetrix).
| | Sample_hyb_protocol | 0.01 mg of labeled and fragmented cRNA were hybridized to the mouse genome MOE430 2.0 array (Affymetrix).
| | Sample_scan_protocol | Raw expression data were analyzed using GCOS 1.4 (Affymetrix). Data were normalized to a target intensity of 500 to account for differences in global chip intensity.
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Roy,,Blum
| | Sample_contact_email | blumroy@gmail.com
| | Sample_contact_phone | 212-263-8129
| | Sample_contact_fax | 212-263-8139
| | Sample_contact_laboratory | Alan Frey Lab
| | Sample_contact_department | Cell Biology
| | Sample_contact_institute | NYU Medical Center
| | Sample_contact_address | 550 First Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.med.nyu.edu/biosketch/freya01/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851981/suppl/GSM851981_Cm_T20.CEL.gz
| | Sample_series_id | GSE34618
| | Sample_data_row_count | 45101
| | |
|
GSM851982 | GPL1261 |
|
ConA-activated CD8+ Central memory T cells at T24, biological rep1
|
CD8+ Central memory T cells after activation with 0.005 ugr Con A
|
source tissue: Spleen
cell type: CD8+ Central memory T
treatment: ConA
time: 24 h
gender: male
age: 62 wk
strain: C57BL/6
|
Gene expression data from CD8+ Central memory T cells after activation with Con A
|
| Sample_geo_accession | GSM851982
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Dec 21 2011
| | Sample_last_update_date | May 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were collected and cultured (0.5 x106 cells/well) in 10% complete RPMI media supplemented with 0.005 ugr Con A.
| | Sample_growth_protocol_ch1 | Ten spleens were pooled from 62 week old C57BL/6 male mice and enriched for CD8+ T cells by negative selection using a cocktail of biotinylated antibodies to deplete CD4, CD11c, CD11b, MHC-II, B220, and NK cells. Cells were then stained for CD8, CD44, CD62L, and CD127 and sorted (using a iCyt Reflection parallel cell sorter).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol Reagent (Invitrogen) followed by RNeasy clean-up kit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples with a RNA Integrity Number (RIN) >7.0 were considered suitable for labeling and 20 ng were labeled using the GeneChip two-cycle target labeling kit (Affymetrix).
| | Sample_hyb_protocol | 0.01 mg of labeled and fragmented cRNA were hybridized to the mouse genome MOE430 2.0 array (Affymetrix).
| | Sample_scan_protocol | Raw expression data were analyzed using GCOS 1.4 (Affymetrix). Data were normalized to a target intensity of 500 to account for differences in global chip intensity.
| | Sample_data_processing | MAS 5.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Roy,,Blum
| | Sample_contact_email | blumroy@gmail.com
| | Sample_contact_phone | 212-263-8129
| | Sample_contact_fax | 212-263-8139
| | Sample_contact_laboratory | Alan Frey Lab
| | Sample_contact_department | Cell Biology
| | Sample_contact_institute | NYU Medical Center
| | Sample_contact_address | 550 First Avenue
| | Sample_contact_city | New York
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.med.nyu.edu/biosketch/freya01/
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM851nnn/GSM851982/suppl/GSM851982_Cm_T24.CEL.gz
| | Sample_series_id | GSE34618
| | Sample_data_row_count | 45101
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