Result

Search results for the GEO ID: GSE34761

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Title

Source name

Description

Characteristics

GSM854784

GPL1261

iPS clone 1 derivated in the presence of ascorbic acid

iPS cells, with ascorbic acid

cell type: induced pluripotent stem cells (iPSCs) treatment: ascorbic acid

MS9 Biological rep 1 of 4.

Sample_geo_accession	GSM854784
Sample_status	Public on Feb 01 2012
Sample_submission_date	Dec 28 2011
Sample_last_update_date	Feb 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	iPS cells were derivated from the reprogrammable mouse MEFs in the presence or absence of ascorbic acid.
Sample_growth_protocol_ch1	iPS cells were derivated in ES cell medium (DMEM with FBS, L-Glutamin, penicillin-streptomycin, non-essential amino acids, beta-mercaptoethanol, LIF, doxycycline, in the presence or absence of ascorbic acid) on irradiated MEF feeder cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	iPS cells grown on 35mm dishes were harvested when they reached about 50% confluency and preplated on non-gelatinized T25 flasks for 45 minutes to remove feeder cells. Cells were spun down and the pellet used for isolation of total RNA using the miRNeasy Mini Kit (QIAGEN) without DNase digestion. RNA was eluted from the columns using 50 ml RNase-free water or TE buffer, pH7.5 (10 mM Tris-HCl and 0.1 mM EDTA) and quantified using Nanodrop (Nanodrop Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA probes were synthesized using Affymetrix one-cycle target labeling and control reagents and the ENZO bioarray high-yield RNA transcript labeling kit following the manufacturer's instructions.
Sample_hyb_protocol	Microarray chip type= Affymetrix GeneChip mouse genome 430 2.0. Affymetrix hybridization oven model 645 and fluidic station model 450. Fluidic protocol was EukGE-WS2v5_450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G controlled by GCOS.
Sample_data_processing = The data were analyzed with Affymetrix GCOS (Library file	mouse genome 430 2.0) using the statistic algorithm (alpha1=0.05, alpha2=0.065, tau=0.015). Scaling target value=150.
Sample_platform_id	GPL1261
Sample_contact_name	Toshi,,Shioda
Sample_contact_email	tshioda@partners.org
Sample_contact_phone	(617) 726-3425
Sample_contact_laboratory	Molecular Profiling Lab
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	MGH Cancer Center
Sample_contact_address	Building 149 - 7th Floor, 13th Street
Sample_contact_city	Charlestown
Sample_contact_state	MA
Sample_contact_zip/postal_code	02129
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854784/suppl/GSM854784.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854784/suppl/GSM854784.CHP.gz
Sample_series_id	GSE34761
Sample_data_row_count	45101

GSM854785

GPL1261

iPS clone 2 derivated in the presence of ascorbic acid

iPS cells, with ascorbic acid

cell type: induced pluripotent stem cells (iPSCs) treatment: ascorbic acid

MS10 Biological rep 2 of 4.

Sample_geo_accession	GSM854785
Sample_status	Public on Feb 01 2012
Sample_submission_date	Dec 28 2011
Sample_last_update_date	Feb 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	iPS cells were derivated from the reprogrammable mouse MEFs in the presence or absence of ascorbic acid.
Sample_growth_protocol_ch1	iPS cells were derivated in ES cell medium (DMEM with FBS, L-Glutamin, penicillin-streptomycin, non-essential amino acids, beta-mercaptoethanol, LIF, doxycycline, in the presence or absence of ascorbic acid) on irradiated MEF feeder cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	iPS cells grown on 35mm dishes were harvested when they reached about 50% confluency and preplated on non-gelatinized T25 flasks for 45 minutes to remove feeder cells. Cells were spun down and the pellet used for isolation of total RNA using the miRNeasy Mini Kit (QIAGEN) without DNase digestion. RNA was eluted from the columns using 50 ml RNase-free water or TE buffer, pH7.5 (10 mM Tris-HCl and 0.1 mM EDTA) and quantified using Nanodrop (Nanodrop Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA probes were synthesized using Affymetrix one-cycle target labeling and control reagents and the ENZO bioarray high-yield RNA transcript labeling kit following the manufacturer's instructions.
Sample_hyb_protocol	Microarray chip type= Affymetrix GeneChip mouse genome 430 2.0. Affymetrix hybridization oven model 645 and fluidic station model 450. Fluidic protocol was EukGE-WS2v5_450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G controlled by GCOS.
Sample_data_processing = The data were analyzed with Affymetrix GCOS (Library file	mouse genome 430 2.0) using the statistic algorithm (alpha1=0.05, alpha2=0.065, tau=0.015). Scaling target value=150.
Sample_platform_id	GPL1261
Sample_contact_name	Toshi,,Shioda
Sample_contact_email	tshioda@partners.org
Sample_contact_phone	(617) 726-3425
Sample_contact_laboratory	Molecular Profiling Lab
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	MGH Cancer Center
Sample_contact_address	Building 149 - 7th Floor, 13th Street
Sample_contact_city	Charlestown
Sample_contact_state	MA
Sample_contact_zip/postal_code	02129
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854785/suppl/GSM854785.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854785/suppl/GSM854785.CHP.gz
Sample_series_id	GSE34761
Sample_data_row_count	45101

GSM854786

GPL1261

iPS clone 3 derivated in the presence of ascorbic acid

iPS cells, with ascorbic acid

cell type: induced pluripotent stem cells (iPSCs) treatment: ascorbic acid

MS11 Biological rep 3 of 4.

Sample_geo_accession	GSM854786
Sample_status	Public on Feb 01 2012
Sample_submission_date	Dec 28 2011
Sample_last_update_date	Feb 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	iPS cells were derivated from the reprogrammable mouse MEFs in the presence or absence of ascorbic acid.
Sample_growth_protocol_ch1	iPS cells were derivated in ES cell medium (DMEM with FBS, L-Glutamin, penicillin-streptomycin, non-essential amino acids, beta-mercaptoethanol, LIF, doxycycline, in the presence or absence of ascorbic acid) on irradiated MEF feeder cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	iPS cells grown on 35mm dishes were harvested when they reached about 50% confluency and preplated on non-gelatinized T25 flasks for 45 minutes to remove feeder cells. Cells were spun down and the pellet used for isolation of total RNA using the miRNeasy Mini Kit (QIAGEN) without DNase digestion. RNA was eluted from the columns using 50 ml RNase-free water or TE buffer, pH7.5 (10 mM Tris-HCl and 0.1 mM EDTA) and quantified using Nanodrop (Nanodrop Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA probes were synthesized using Affymetrix one-cycle target labeling and control reagents and the ENZO bioarray high-yield RNA transcript labeling kit following the manufacturer's instructions.
Sample_hyb_protocol	Microarray chip type= Affymetrix GeneChip mouse genome 430 2.0. Affymetrix hybridization oven model 645 and fluidic station model 450. Fluidic protocol was EukGE-WS2v5_450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G controlled by GCOS.
Sample_data_processing = The data were analyzed with Affymetrix GCOS (Library file	mouse genome 430 2.0) using the statistic algorithm (alpha1=0.05, alpha2=0.065, tau=0.015). Scaling target value=150.
Sample_platform_id	GPL1261
Sample_contact_name	Toshi,,Shioda
Sample_contact_email	tshioda@partners.org
Sample_contact_phone	(617) 726-3425
Sample_contact_laboratory	Molecular Profiling Lab
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	MGH Cancer Center
Sample_contact_address	Building 149 - 7th Floor, 13th Street
Sample_contact_city	Charlestown
Sample_contact_state	MA
Sample_contact_zip/postal_code	02129
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854786/suppl/GSM854786.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854786/suppl/GSM854786.CHP.gz
Sample_series_id	GSE34761
Sample_data_row_count	45101

GSM854787

GPL1261

iPS clone 4 derivated in the presence of ascorbic acid

iPS cells, with ascorbic acid

cell type: induced pluripotent stem cells (iPSCs) treatment: ascorbic acid

MS12 Biological rep 4 of 4.

Sample_geo_accession	GSM854787
Sample_status	Public on Feb 01 2012
Sample_submission_date	Dec 28 2011
Sample_last_update_date	Feb 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	iPS cells were derivated from the reprogrammable mouse MEFs in the presence or absence of ascorbic acid.
Sample_growth_protocol_ch1	iPS cells were derivated in ES cell medium (DMEM with FBS, L-Glutamin, penicillin-streptomycin, non-essential amino acids, beta-mercaptoethanol, LIF, doxycycline, in the presence or absence of ascorbic acid) on irradiated MEF feeder cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	iPS cells grown on 35mm dishes were harvested when they reached about 50% confluency and preplated on non-gelatinized T25 flasks for 45 minutes to remove feeder cells. Cells were spun down and the pellet used for isolation of total RNA using the miRNeasy Mini Kit (QIAGEN) without DNase digestion. RNA was eluted from the columns using 50 ml RNase-free water or TE buffer, pH7.5 (10 mM Tris-HCl and 0.1 mM EDTA) and quantified using Nanodrop (Nanodrop Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA probes were synthesized using Affymetrix one-cycle target labeling and control reagents and the ENZO bioarray high-yield RNA transcript labeling kit following the manufacturer's instructions.
Sample_hyb_protocol	Microarray chip type= Affymetrix GeneChip mouse genome 430 2.0. Affymetrix hybridization oven model 645 and fluidic station model 450. Fluidic protocol was EukGE-WS2v5_450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G controlled by GCOS.
Sample_data_processing = The data were analyzed with Affymetrix GCOS (Library file	mouse genome 430 2.0) using the statistic algorithm (alpha1=0.05, alpha2=0.065, tau=0.015). Scaling target value=150.
Sample_platform_id	GPL1261
Sample_contact_name	Toshi,,Shioda
Sample_contact_email	tshioda@partners.org
Sample_contact_phone	(617) 726-3425
Sample_contact_laboratory	Molecular Profiling Lab
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	MGH Cancer Center
Sample_contact_address	Building 149 - 7th Floor, 13th Street
Sample_contact_city	Charlestown
Sample_contact_state	MA
Sample_contact_zip/postal_code	02129
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854787/suppl/GSM854787.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854787/suppl/GSM854787.CHP.gz
Sample_series_id	GSE34761
Sample_data_row_count	45101

GSM854788

GPL1261

iPS clone 1 derivated in the absence of ascorbic acid

iPS cells, without ascorbic acid

cell type: induced pluripotent stem cells (iPSCs) treatment: none

MS13 Biological rep 1 of 4.

Sample_geo_accession	GSM854788
Sample_status	Public on Feb 01 2012
Sample_submission_date	Dec 28 2011
Sample_last_update_date	Feb 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	iPS cells were derivated from the reprogrammable mouse MEFs in the presence or absence of ascorbic acid.
Sample_growth_protocol_ch1	iPS cells were derivated in ES cell medium (DMEM with FBS, L-Glutamin, penicillin-streptomycin, non-essential amino acids, beta-mercaptoethanol, LIF, doxycycline, in the presence or absence of ascorbic acid) on irradiated MEF feeder cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	iPS cells grown on 35mm dishes were harvested when they reached about 50% confluency and preplated on non-gelatinized T25 flasks for 45 minutes to remove feeder cells. Cells were spun down and the pellet used for isolation of total RNA using the miRNeasy Mini Kit (QIAGEN) without DNase digestion. RNA was eluted from the columns using 50 ml RNase-free water or TE buffer, pH7.5 (10 mM Tris-HCl and 0.1 mM EDTA) and quantified using Nanodrop (Nanodrop Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA probes were synthesized using Affymetrix one-cycle target labeling and control reagents and the ENZO bioarray high-yield RNA transcript labeling kit following the manufacturer's instructions.
Sample_hyb_protocol	Microarray chip type= Affymetrix GeneChip mouse genome 430 2.0. Affymetrix hybridization oven model 645 and fluidic station model 450. Fluidic protocol was EukGE-WS2v5_450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G controlled by GCOS.
Sample_data_processing = The data were analyzed with Affymetrix GCOS (Library file	mouse genome 430 2.0) using the statistic algorithm (alpha1=0.05, alpha2=0.065, tau=0.015). Scaling target value=150.
Sample_platform_id	GPL1261
Sample_contact_name	Toshi,,Shioda
Sample_contact_email	tshioda@partners.org
Sample_contact_phone	(617) 726-3425
Sample_contact_laboratory	Molecular Profiling Lab
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	MGH Cancer Center
Sample_contact_address	Building 149 - 7th Floor, 13th Street
Sample_contact_city	Charlestown
Sample_contact_state	MA
Sample_contact_zip/postal_code	02129
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854788/suppl/GSM854788.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854788/suppl/GSM854788.CHP.gz
Sample_series_id	GSE34761
Sample_data_row_count	45101

GSM854789

GPL1261

iPS clone 2 derivated in the absence of ascorbic acid

iPS cells, without ascorbic acid

cell type: induced pluripotent stem cells (iPSCs) treatment: none

MS14 Biological rep 2 of 4.

Sample_geo_accession	GSM854789
Sample_status	Public on Feb 01 2012
Sample_submission_date	Dec 28 2011
Sample_last_update_date	Feb 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	iPS cells were derivated from the reprogrammable mouse MEFs in the presence or absence of ascorbic acid.
Sample_growth_protocol_ch1	iPS cells were derivated in ES cell medium (DMEM with FBS, L-Glutamin, penicillin-streptomycin, non-essential amino acids, beta-mercaptoethanol, LIF, doxycycline, in the presence or absence of ascorbic acid) on irradiated MEF feeder cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	iPS cells grown on 35mm dishes were harvested when they reached about 50% confluency and preplated on non-gelatinized T25 flasks for 45 minutes to remove feeder cells. Cells were spun down and the pellet used for isolation of total RNA using the miRNeasy Mini Kit (QIAGEN) without DNase digestion. RNA was eluted from the columns using 50 ml RNase-free water or TE buffer, pH7.5 (10 mM Tris-HCl and 0.1 mM EDTA) and quantified using Nanodrop (Nanodrop Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA probes were synthesized using Affymetrix one-cycle target labeling and control reagents and the ENZO bioarray high-yield RNA transcript labeling kit following the manufacturer's instructions.
Sample_hyb_protocol	Microarray chip type= Affymetrix GeneChip mouse genome 430 2.0. Affymetrix hybridization oven model 645 and fluidic station model 450. Fluidic protocol was EukGE-WS2v5_450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G controlled by GCOS.
Sample_data_processing = The data were analyzed with Affymetrix GCOS (Library file	mouse genome 430 2.0) using the statistic algorithm (alpha1=0.05, alpha2=0.065, tau=0.015). Scaling target value=150.
Sample_platform_id	GPL1261
Sample_contact_name	Toshi,,Shioda
Sample_contact_email	tshioda@partners.org
Sample_contact_phone	(617) 726-3425
Sample_contact_laboratory	Molecular Profiling Lab
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	MGH Cancer Center
Sample_contact_address	Building 149 - 7th Floor, 13th Street
Sample_contact_city	Charlestown
Sample_contact_state	MA
Sample_contact_zip/postal_code	02129
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854789/suppl/GSM854789.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854789/suppl/GSM854789.CHP.gz
Sample_series_id	GSE34761
Sample_data_row_count	45101

GSM854790

GPL1261

iPS clone 3 derivated in the absence of ascorbic acid

iPS cells, without ascorbic acid

cell type: induced pluripotent stem cells (iPSCs) treatment: none

MS15 Biological rep 3 of 4.

Sample_geo_accession	GSM854790
Sample_status	Public on Feb 01 2012
Sample_submission_date	Dec 28 2011
Sample_last_update_date	Feb 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	iPS cells were derivated from the reprogrammable mouse MEFs in the presence or absence of ascorbic acid.
Sample_growth_protocol_ch1	iPS cells were derivated in ES cell medium (DMEM with FBS, L-Glutamin, penicillin-streptomycin, non-essential amino acids, beta-mercaptoethanol, LIF, doxycycline, in the presence or absence of ascorbic acid) on irradiated MEF feeder cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	iPS cells grown on 35mm dishes were harvested when they reached about 50% confluency and preplated on non-gelatinized T25 flasks for 45 minutes to remove feeder cells. Cells were spun down and the pellet used for isolation of total RNA using the miRNeasy Mini Kit (QIAGEN) without DNase digestion. RNA was eluted from the columns using 50 ml RNase-free water or TE buffer, pH7.5 (10 mM Tris-HCl and 0.1 mM EDTA) and quantified using Nanodrop (Nanodrop Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA probes were synthesized using Affymetrix one-cycle target labeling and control reagents and the ENZO bioarray high-yield RNA transcript labeling kit following the manufacturer's instructions.
Sample_hyb_protocol	Microarray chip type= Affymetrix GeneChip mouse genome 430 2.0. Affymetrix hybridization oven model 645 and fluidic station model 450. Fluidic protocol was EukGE-WS2v5_450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G controlled by GCOS.
Sample_data_processing = The data were analyzed with Affymetrix GCOS (Library file	mouse genome 430 2.0) using the statistic algorithm (alpha1=0.05, alpha2=0.065, tau=0.015). Scaling target value=150.
Sample_platform_id	GPL1261
Sample_contact_name	Toshi,,Shioda
Sample_contact_email	tshioda@partners.org
Sample_contact_phone	(617) 726-3425
Sample_contact_laboratory	Molecular Profiling Lab
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	MGH Cancer Center
Sample_contact_address	Building 149 - 7th Floor, 13th Street
Sample_contact_city	Charlestown
Sample_contact_state	MA
Sample_contact_zip/postal_code	02129
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854790/suppl/GSM854790.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854790/suppl/GSM854790.CHP.gz
Sample_series_id	GSE34761
Sample_data_row_count	45101

GSM854791

GPL1261

iPS clone 4 derivated in the absence of ascorbic acid

iPS cells, without ascorbic acid

cell type: induced pluripotent stem cells (iPSCs) treatment: none

MS16 Biological rep 4 of 4.

Sample_geo_accession	GSM854791
Sample_status	Public on Feb 01 2012
Sample_submission_date	Dec 28 2011
Sample_last_update_date	Feb 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	iPS cells were derivated from the reprogrammable mouse MEFs in the presence or absence of ascorbic acid.
Sample_growth_protocol_ch1	iPS cells were derivated in ES cell medium (DMEM with FBS, L-Glutamin, penicillin-streptomycin, non-essential amino acids, beta-mercaptoethanol, LIF, doxycycline, in the presence or absence of ascorbic acid) on irradiated MEF feeder cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	iPS cells grown on 35mm dishes were harvested when they reached about 50% confluency and preplated on non-gelatinized T25 flasks for 45 minutes to remove feeder cells. Cells were spun down and the pellet used for isolation of total RNA using the miRNeasy Mini Kit (QIAGEN) without DNase digestion. RNA was eluted from the columns using 50 ml RNase-free water or TE buffer, pH7.5 (10 mM Tris-HCl and 0.1 mM EDTA) and quantified using Nanodrop (Nanodrop Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA probes were synthesized using Affymetrix one-cycle target labeling and control reagents and the ENZO bioarray high-yield RNA transcript labeling kit following the manufacturer's instructions.
Sample_hyb_protocol	Microarray chip type= Affymetrix GeneChip mouse genome 430 2.0. Affymetrix hybridization oven model 645 and fluidic station model 450. Fluidic protocol was EukGE-WS2v5_450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G controlled by GCOS.
Sample_data_processing = The data were analyzed with Affymetrix GCOS (Library file	mouse genome 430 2.0) using the statistic algorithm (alpha1=0.05, alpha2=0.065, tau=0.015). Scaling target value=150.
Sample_platform_id	GPL1261
Sample_contact_name	Toshi,,Shioda
Sample_contact_email	tshioda@partners.org
Sample_contact_phone	(617) 726-3425
Sample_contact_laboratory	Molecular Profiling Lab
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	MGH Cancer Center
Sample_contact_address	Building 149 - 7th Floor, 13th Street
Sample_contact_city	Charlestown
Sample_contact_state	MA
Sample_contact_zip/postal_code	02129
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854791/suppl/GSM854791.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854791/suppl/GSM854791.CHP.gz
Sample_series_id	GSE34761
Sample_data_row_count	45101

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