Search results for the GEO ID: GSE34765 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM854826 | GPL1261 |
|
Cerebellum_WildType_REP1
|
cerebellum wild type
|
hybrid C57BL/6 x CBA
genotype/variation: Wild Type
tissue: cerebellum
|
Wt1_Mousegenome430_2.0.CEL
Gene expression data from, wild type cerebellum cells replicate 1
|
Sample_geo_accession | GSM854826
| Sample_status | Public on Sep 30 2012
| Sample_submission_date | Dec 29 2011
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cerebellar tissue from wild type or transgenic mice was dissected and immediatelly frozen.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was prepared using TRIzol (Invitrogen) and the RNeasy Mini Kit (Qiagen). RNA was quantified and the RNA quality was assessed with a 2100 Bioanalyzer from Agilent Technologies
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 4 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg of biotinylated cRNA were hybridized to Affymetrix chips (GeneChip Mouse Genome 430 2.0 Array). Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix).
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | GeneChip intensities were background-corrected, normalized and sumarized by RMA method, using the “Affy” package from Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Juan,Carlos,Oliveros
| Sample_contact_institute | CNB, CSIC
| Sample_contact_address | Darwin 3
| Sample_contact_city | Cantoblanco
| Sample_contact_state | Madrid
| Sample_contact_zip/postal_code | 28049
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854826/suppl/GSM854826.CEL.gz
| Sample_series_id | GSE34765
| Sample_data_row_count | 45101
| |
|
GSM854827 | GPL1261 |
|
Cerebellum_WildType_REP2
|
cerebellum wild type
|
hybrid C57BL/6 x CBA
genotype/variation: Wild Type
tissue: cerebellum
|
Wt2_Mousegenome430_2.0.CEL
Gene expression data from, wild type cerebellum cells replicate 2
|
Sample_geo_accession | GSM854827
| Sample_status | Public on Sep 30 2012
| Sample_submission_date | Dec 29 2011
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cerebellar tissue from wild type or transgenic mice was dissected and immediatelly frozen.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was prepared using TRIzol (Invitrogen) and the RNeasy Mini Kit (Qiagen). RNA was quantified and the RNA quality was assessed with a 2100 Bioanalyzer from Agilent Technologies
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 4 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg of biotinylated cRNA were hybridized to Affymetrix chips (GeneChip Mouse Genome 430 2.0 Array). Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix).
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | GeneChip intensities were background-corrected, normalized and sumarized by RMA method, using the “Affy” package from Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Juan,Carlos,Oliveros
| Sample_contact_institute | CNB, CSIC
| Sample_contact_address | Darwin 3
| Sample_contact_city | Cantoblanco
| Sample_contact_state | Madrid
| Sample_contact_zip/postal_code | 28049
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854827/suppl/GSM854827.CEL.gz
| Sample_series_id | GSE34765
| Sample_data_row_count | 45101
| |
|
GSM854828 | GPL1261 |
|
Cerebellum_WildType_REP3
|
cerebellum wild type
|
hybrid C57BL/6 x CBA
genotype/variation: Wild Type
tissue: cerebellum
|
Wt3_Mousegenome430_2.0.CEL
Gene expression data from, wild type cerebellum cells replicate 3
|
Sample_geo_accession | GSM854828
| Sample_status | Public on Sep 30 2012
| Sample_submission_date | Dec 29 2011
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cerebellar tissue from wild type or transgenic mice was dissected and immediatelly frozen.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was prepared using TRIzol (Invitrogen) and the RNeasy Mini Kit (Qiagen). RNA was quantified and the RNA quality was assessed with a 2100 Bioanalyzer from Agilent Technologies
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 4 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg of biotinylated cRNA were hybridized to Affymetrix chips (GeneChip Mouse Genome 430 2.0 Array). Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix).
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | GeneChip intensities were background-corrected, normalized and sumarized by RMA method, using the “Affy” package from Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Juan,Carlos,Oliveros
| Sample_contact_institute | CNB, CSIC
| Sample_contact_address | Darwin 3
| Sample_contact_city | Cantoblanco
| Sample_contact_state | Madrid
| Sample_contact_zip/postal_code | 28049
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854828/suppl/GSM854828.CEL.gz
| Sample_series_id | GSE34765
| Sample_data_row_count | 45101
| |
|
GSM854829 | GPL1261 |
|
Cerebellum_Transgenic_REP1
|
cerebellum transgenic
|
hybrid C57BL/6 x CBA
genotype/variation: daDREAM
tissue: cerebellum
|
L33-1_Mousegenome430_2.0.CEL
Gene expression data from, transgenic (daDREAM) cerebellum cells replicate 1
|
Sample_geo_accession | GSM854829
| Sample_status | Public on Sep 30 2012
| Sample_submission_date | Dec 29 2011
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cerebellar tissue from wild type or transgenic mice was dissected and immediatelly frozen.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was prepared using TRIzol (Invitrogen) and the RNeasy Mini Kit (Qiagen). RNA was quantified and the RNA quality was assessed with a 2100 Bioanalyzer from Agilent Technologies
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 4 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg of biotinylated cRNA were hybridized to Affymetrix chips (GeneChip Mouse Genome 430 2.0 Array). Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix).
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | GeneChip intensities were background-corrected, normalized and sumarized by RMA method, using the “Affy” package from Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Juan,Carlos,Oliveros
| Sample_contact_institute | CNB, CSIC
| Sample_contact_address | Darwin 3
| Sample_contact_city | Cantoblanco
| Sample_contact_state | Madrid
| Sample_contact_zip/postal_code | 28049
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854829/suppl/GSM854829.CEL.gz
| Sample_series_id | GSE34765
| Sample_data_row_count | 45101
| |
|
GSM854830 | GPL1261 |
|
Cerebellum_Transgenic_REP2
|
cerebellum transgenic
|
hybrid C57BL/6 x CBA
genotype/variation: daDREAM
tissue: cerebellum
|
L33-2_Mousegenome430_2.0.CEL
Gene expression data from, transgenic (daDREAM) cerebellum cells replicate 2
|
Sample_geo_accession | GSM854830
| Sample_status | Public on Sep 30 2012
| Sample_submission_date | Dec 29 2011
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cerebellar tissue from wild type or transgenic mice was dissected and immediatelly frozen.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was prepared using TRIzol (Invitrogen) and the RNeasy Mini Kit (Qiagen). RNA was quantified and the RNA quality was assessed with a 2100 Bioanalyzer from Agilent Technologies
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 4 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg of biotinylated cRNA were hybridized to Affymetrix chips (GeneChip Mouse Genome 430 2.0 Array). Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix).
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | GeneChip intensities were background-corrected, normalized and sumarized by RMA method, using the “Affy” package from Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Juan,Carlos,Oliveros
| Sample_contact_institute | CNB, CSIC
| Sample_contact_address | Darwin 3
| Sample_contact_city | Cantoblanco
| Sample_contact_state | Madrid
| Sample_contact_zip/postal_code | 28049
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854830/suppl/GSM854830.CEL.gz
| Sample_series_id | GSE34765
| Sample_data_row_count | 45101
| |
|
GSM854831 | GPL1261 |
|
Cerebellum_Transgenic_REP3
|
cerebellum transgenic
|
hybrid C57BL/6 x CBA
genotype/variation: daDREAM
tissue: cerebellum
|
L33-3_Mousegenome430_2.0.CEL
Gene expression data from, transgenic (daDREAM) cerebellum cells replicate 3
|
Sample_geo_accession | GSM854831
| Sample_status | Public on Sep 30 2012
| Sample_submission_date | Dec 29 2011
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cerebellar tissue from wild type or transgenic mice was dissected and immediatelly frozen.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was prepared using TRIzol (Invitrogen) and the RNeasy Mini Kit (Qiagen). RNA was quantified and the RNA quality was assessed with a 2100 Bioanalyzer from Agilent Technologies
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 4 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg of biotinylated cRNA were hybridized to Affymetrix chips (GeneChip Mouse Genome 430 2.0 Array). Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix).
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | GeneChip intensities were background-corrected, normalized and sumarized by RMA method, using the “Affy” package from Bioconductor.
| Sample_platform_id | GPL1261
| Sample_contact_name | Juan,Carlos,Oliveros
| Sample_contact_institute | CNB, CSIC
| Sample_contact_address | Darwin 3
| Sample_contact_city | Cantoblanco
| Sample_contact_state | Madrid
| Sample_contact_zip/postal_code | 28049
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM854nnn/GSM854831/suppl/GSM854831.CEL.gz
| Sample_series_id | GSE34765
| Sample_data_row_count | 45101
| |
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