Result

Search results for the GEO ID: GSE34789

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM855438

GPL570

Calu-3 18 hrs non-stretch

Calu-3 cells without cylcic mechanical stretch

cell line: Calu-3 cell type: human pulmonary epithelial cells

Calu-3 human pulmonary epithelial cells without 18 hours of cyclic mechanical stretch

Sample_geo_accession	GSM855438
Sample_status	Public on Jan 24 2012
Sample_submission_date	Dec 30 2011
Sample_last_update_date	Jan 25 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Calu-3 human bronchial epithelial cells were plated on BioFlex culture plates-collagen type I (BF-3001C; FlexCell International) and allowed to attach and grow to ~80% conﬂuence. The medium was changed to MEM plus 10% FBS. Plates were then placed on a FlexCell FX-4000T Tension Plus System and stretched at 20% stretch maximum, 0.7% stretch minimum, sine wave 2 s, on 2 s off.
Sample_growth_protocol_ch1	Human bronchial epithelial cells (Calu-3 or A549) were cultured as described previously (Haeberle et al., 2008; Liedtke et al., 2005).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen spin column protocol for total RNA extraction was performed according to manufacturer instruction
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human genome array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
Sample_data_processing	The data were analyzed with dCHIP (Build 17. December 2010) using default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Michael,,Koeppen
Sample_contact_institute	Univeristy of Colorado - Anschutz Medical Campus
Sample_contact_address	12700 E19th Ave
Sample_contact_city	Aurora
Sample_contact_zip/postal_code	80045
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM855nnn/GSM855438/suppl/GSM855438_Calu3_Nonstretch_18hr_HGU133_Plus_2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM855nnn/GSM855438/suppl/GSM855438_Calu3_Nonstretch_18hr_HGU133_Plus_2.CHP.gz
Sample_series_id	GSE34789
Sample_data_row_count	54675

GSM855439

GPL570

Calu-3 18 hrs stretch

Calu-3 cells with cylcic mechanical stretch

cell line: Calu-3 cell type: human pulmonary epithelial cells

Calu-3 human pulmonary epithelial cells with18 hours of cyclic mechanical stretch

Sample_geo_accession	GSM855439
Sample_status	Public on Jan 24 2012
Sample_submission_date	Dec 30 2011
Sample_last_update_date	Jan 25 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Calu-3 human bronchial epithelial cells were plated on BioFlex culture plates-collagen type I (BF-3001C; FlexCell International) and allowed to attach and grow to ~80% conﬂuence. The medium was changed to MEM plus 10% FBS. Plates were then placed on a FlexCell FX-4000T Tension Plus System and stretched at 20% stretch maximum, 0.7% stretch minimum, sine wave 2 s, on 2 s off.
Sample_growth_protocol_ch1	Human bronchial epithelial cells (Calu-3 or A549) were cultured as described previously (Haeberle et al., 2008; Liedtke et al., 2005).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen spin column protocol for total RNA extraction was performed according to manufacturer instruction
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human genome array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
Sample_data_processing	The data were analyzed with dCHIP (Build 17. December 2010) using default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Michael,,Koeppen
Sample_contact_institute	Univeristy of Colorado - Anschutz Medical Campus
Sample_contact_address	12700 E19th Ave
Sample_contact_city	Aurora
Sample_contact_zip/postal_code	80045
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM855nnn/GSM855439/suppl/GSM855439_Calu3_Stretch_18hr_HGU133_Plus_2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM855nnn/GSM855439/suppl/GSM855439_Calu3_Stretch_18hr_HGU133_Plus_2.CHP.gz
Sample_series_id	GSE34789
Sample_data_row_count	54675

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