Search results for the GEO ID: GSE34973 |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM859656 | GPL1261 |
|
OP9M2 biological rep1
|
Supportive mesenchymal stem cell line OP9M2
|
cell line: OP9M2
cell type: Mesenchymal Stem cells
|
Gene expression from OP9M2 Mesenchymal stem cells
|
Sample_geo_accession | GSM859656
| Sample_status | Public on Jan 31 2013
| Sample_submission_date | Jan 10 2012
| Sample_last_update_date | Jan 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Stromal cells were irradiated (2000 rad) and plated (25,000 cells/ cm2) on tissue culture treated wells 48 hrs before harvest
| Sample_growth_protocol_ch1 | OP9M2 Stromal cells were cultured in stroma media containing α-MEM (GIBCO/Invitrogen), 20% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO/Invitrogen). BFC012 was cultured in stroma media containing α-MEM (GIBCO/Invitrogen), 10% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO/Invitrogen)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified from one 10 cm culture plate cells that were using QIAshredder and RNEasy Mini Kit (QIAGEN).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocol
| Sample_hyb_protocol | standard Affymetrix protocol
| Sample_scan_protocol | standard Affymetrix protocol
| Sample_data_processing | CEL files were normalised using RMA method using the affy package from Bioconductor version 2.7
| Sample_platform_id | GPL1261
| Sample_contact_name | Mattias,,Magnusson
| Sample_contact_email | mattias.magnusson@med.lu.se
| Sample_contact_department | MCDB
| Sample_contact_institute | UCLA
| Sample_contact_address | 621 Charles E Young Drive South, 2204
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90092
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM859nnn/GSM859656/suppl/GSM859656_OP9M2_R1.CEL.gz
| Sample_series_id | GSE34973
| Sample_series_id | GSE34974
| Sample_data_row_count | 45101
| |
|
GSM859657 | GPL1261 |
|
OP9M2 biological rep2
|
Supportive mesenchymal stem cell line OP9M2
|
cell line: OP9M2
cell type: Mesenchymal Stem cells
|
Gene expression from OP9M2 Mesenchymal stem cells
|
Sample_geo_accession | GSM859657
| Sample_status | Public on Jan 31 2013
| Sample_submission_date | Jan 10 2012
| Sample_last_update_date | Jan 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Stromal cells were irradiated (2000 rad) and plated (25,000 cells/ cm2) on tissue culture treated wells 48 hrs before harvest
| Sample_growth_protocol_ch1 | OP9M2 Stromal cells were cultured in stroma media containing α-MEM (GIBCO/Invitrogen), 20% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO/Invitrogen). BFC012 was cultured in stroma media containing α-MEM (GIBCO/Invitrogen), 10% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO/Invitrogen)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified from one 10 cm culture plate cells that were using QIAshredder and RNEasy Mini Kit (QIAGEN).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocol
| Sample_hyb_protocol | standard Affymetrix protocol
| Sample_scan_protocol | standard Affymetrix protocol
| Sample_data_processing | CEL files were normalised using RMA method using the affy package from Bioconductor version 2.7
| Sample_platform_id | GPL1261
| Sample_contact_name | Mattias,,Magnusson
| Sample_contact_email | mattias.magnusson@med.lu.se
| Sample_contact_department | MCDB
| Sample_contact_institute | UCLA
| Sample_contact_address | 621 Charles E Young Drive South, 2204
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90092
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM859nnn/GSM859657/suppl/GSM859657_OP9M2_R2.CEL.gz
| Sample_series_id | GSE34973
| Sample_series_id | GSE34974
| Sample_data_row_count | 45101
| |
|
GSM859658 | GPL1261 |
|
BFC012 biological rep1
|
Non supportive fibroblast line BFC012
|
cell line: BFC012
cell type: Fibroblast from fetal liver
|
Gene expression from BFC012
|
Sample_geo_accession | GSM859658
| Sample_status | Public on Jan 31 2013
| Sample_submission_date | Jan 10 2012
| Sample_last_update_date | Jan 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Stromal cells were irradiated (2000 rad) and plated (25,000 cells/ cm2) on tissue culture treated wells 48 hrs before harvest
| Sample_growth_protocol_ch1 | OP9M2 Stromal cells were cultured in stroma media containing α-MEM (GIBCO/Invitrogen), 20% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO/Invitrogen). BFC012 was cultured in stroma media containing α-MEM (GIBCO/Invitrogen), 10% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO/Invitrogen)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified from one 10 cm culture plate cells that were using QIAshredder and RNEasy Mini Kit (QIAGEN).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocol
| Sample_hyb_protocol | standard Affymetrix protocol
| Sample_scan_protocol | standard Affymetrix protocol
| Sample_data_processing | CEL files were normalised using RMA method using the affy package from Bioconductor version 2.7
| Sample_platform_id | GPL1261
| Sample_contact_name | Mattias,,Magnusson
| Sample_contact_email | mattias.magnusson@med.lu.se
| Sample_contact_department | MCDB
| Sample_contact_institute | UCLA
| Sample_contact_address | 621 Charles E Young Drive South, 2204
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90092
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM859nnn/GSM859658/suppl/GSM859658_BFC012_R1.CEL.gz
| Sample_series_id | GSE34973
| Sample_series_id | GSE34974
| Sample_data_row_count | 45101
| |
|
GSM859659 | GPL1261 |
|
BFC012 biological rep2
|
Non supportive fibroblast line BFC012
|
cell line: BFC012
cell type: Fibroblast from fetal liver
|
Gene expression from BFC012
|
Sample_geo_accession | GSM859659
| Sample_status | Public on Jan 31 2013
| Sample_submission_date | Jan 10 2012
| Sample_last_update_date | Jan 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Stromal cells were irradiated (2000 rad) and plated (25,000 cells/ cm2) on tissue culture treated wells 48 hrs before harvest
| Sample_growth_protocol_ch1 | OP9M2 Stromal cells were cultured in stroma media containing α-MEM (GIBCO/Invitrogen), 20% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO/Invitrogen). BFC012 was cultured in stroma media containing α-MEM (GIBCO/Invitrogen), 10% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO/Invitrogen)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified from one 10 cm culture plate cells that were using QIAshredder and RNEasy Mini Kit (QIAGEN).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | standard Affymetrix protocol
| Sample_hyb_protocol | standard Affymetrix protocol
| Sample_scan_protocol | standard Affymetrix protocol
| Sample_data_processing | CEL files were normalised using RMA method using the affy package from Bioconductor version 2.7
| Sample_platform_id | GPL1261
| Sample_contact_name | Mattias,,Magnusson
| Sample_contact_email | mattias.magnusson@med.lu.se
| Sample_contact_department | MCDB
| Sample_contact_institute | UCLA
| Sample_contact_address | 621 Charles E Young Drive South, 2204
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90092
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM859nnn/GSM859659/suppl/GSM859659_BFC012_R2.CEL.gz
| Sample_series_id | GSE34973
| Sample_series_id | GSE34974
| Sample_data_row_count | 45101
| |
|
|
|
Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
Select expression type |
Transcripts profile based on; |
A. Differential status (Up/Down regulation) |
|
|
Regulation type |
|
Fold change |
|
p-value |
|
|
|
B. Absolute calls (Transcribed/Not-detected) |
|
|
Derive calls within/across groups |
Within groups |
|
|
Detection status |
|
Percentage detection |
|
|
Across groups |
|
|
Detection status |
First group: |
- |
Second group:
|
Percentage detection |
First group: |
- |
Second group:
|
|
|
|
Filter results by number of probes |
|
|