Search results for the GEO ID: GSE35159  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM862823 | GPL570 |
|
HEL
|
erythroleukemia
|
expression: EVI1 low AML
cell line: HEL
|
|
| Sample_geo_accession | GSM862823
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862823/suppl/GSM862823_1_HEL.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862824 | GPL570 |
|
HL60
|
AML
|
expression: EVI1 low AML
cell line: HL60
|
|
| Sample_geo_accession | GSM862824
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862824/suppl/GSM862824_2_HL60.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862825 | GPL570 |
|
K052
|
AML
|
expression: EVI1 low AML
cell line: K052
|
|
| Sample_geo_accession | GSM862825
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862825/suppl/GSM862825_3_K052.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862826 | GPL570 |
|
THP1
|
AML
|
expression: EVI1 low AML
cell line: THP1
|
|
| Sample_geo_accession | GSM862826
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862826/suppl/GSM862826_4_THP1.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862827 | GPL570 |
|
FKH-1
|
AML
|
expression: EVI1 low AML
cell line: FKH-1
|
|
| Sample_geo_accession | GSM862827
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862827/suppl/GSM862827_5_FKH1.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862828 | GPL570 |
|
K051
|
AML
|
expression: EVI1 low AML
cell line: K051
|
|
| Sample_geo_accession | GSM862828
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862828/suppl/GSM862828_6_K051.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862829 | GPL570 |
|
NH
|
AML
|
expression: EVI1 low AML
cell line: NH
|
|
| Sample_geo_accession | GSM862829
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862829/suppl/GSM862829_7_NH.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862830 | GPL570 |
|
OIH1
|
AML
|
expression: EVI1 low AML
cell line: OIH1
|
|
| Sample_geo_accession | GSM862830
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862830/suppl/GSM862830_8_OIH1.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862831 | GPL570 |
|
UCSD/AML1
|
AML
|
expression: EVI1 high AML
cell line: UCSD/AML1
|
|
| Sample_geo_accession | GSM862831
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862831/suppl/GSM862831_9_AML1.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862832 | GPL570 |
|
HNT-34
|
AML
|
expression: EVI1 high AML
cell line: HNT-34
|
|
| Sample_geo_accession | GSM862832
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862832/suppl/GSM862832_10_HNT34.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862833 | GPL570 |
|
Kasumi-3
|
AML
|
expression: EVI1 high AML
cell line: Kasumi-3
|
|
| Sample_geo_accession | GSM862833
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862833/suppl/GSM862833_11_Kasumi3.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
|
GSM862834 | GPL570 |
|
MOLM1
|
CML in blast crisis
|
expression: EVI1 high AML
cell line: MOLM1
|
|
| Sample_geo_accession | GSM862834
| | Sample_status | Public on Jan 18 2012
| | Sample_submission_date | Jan 17 2012
| | Sample_last_update_date | Jan 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yusuke,,Saito
| | Sample_contact_institute | Miyazaki University
| | Sample_contact_address | 5200 Kihara, Kiyotake
| | Sample_contact_city | Miyazaki
| | Sample_contact_zip/postal_code | 889-1692
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862834/suppl/GSM862834_12_MOLM1.CEL.gz
| | Sample_series_id | GSE35159
| | Sample_data_row_count | 54675
| | |
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