Search results for the GEO ID: GSE35208  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM862922 | GPL570 |
|
U87-1
|
U87 glioma cells from ATCC cultivated in vitro according to ATCC's protocol
|
cell line: U87 glioma cells
cell type: Late passage cells
|
|
| Sample_geo_accession | GSM862922
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862922/suppl/GSM862922_U87-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862922/suppl/GSM862922_U87-1.CHP.gz
| | Sample_series_id | GSE35169
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862923 | GPL570 |
|
U87-2
|
U87 glioma cells from ATCC cultivated in vitro according to ATCC's protocol
|
cell line: U87 glioma cells
cell type: Late passage cells
|
|
| Sample_geo_accession | GSM862923
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862923/suppl/GSM862923_U87-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862923/suppl/GSM862923_U87-2.CHP.gz
| | Sample_series_id | GSE35169
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862924 | GPL570 |
|
U87-3
|
U87 glioma cells from ATCC cultivated in vitro according to ATCC's protocol
|
cell line: U87 glioma cells
cell type: Late passage cells
|
|
| Sample_geo_accession | GSM862924
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862924/suppl/GSM862924_U87-3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862924/suppl/GSM862924_U87-3.CHP.gz
| | Sample_series_id | GSE35169
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862925 | GPL570 |
|
U87-2M1-1
|
U87-2M1 glioma cells derived from U87 cells subjected to an in vivo experimental lung metastasis assay and propagated in vitro
|
cell line: Invasive subline U87-2M1 derived from U87
cell type: Passage 4 cells, maintained under hypoxia
|
|
| Sample_geo_accession | GSM862925
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862925/suppl/GSM862925_U87-2M1-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862925/suppl/GSM862925_U87-2M1-1.CHP.gz
| | Sample_series_id | GSE35169
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862926 | GPL570 |
|
U87-2M1-2
|
U87-2M1 glioma cells derived from U87 cells subjected to an in vivo experimental lung metastasis assay and propagated in vitro
|
cell line: Invasive subline U87-2M1 derived from U87
cell type: Passage 4 cells, maintained under hypoxia
|
|
| Sample_geo_accession | GSM862926
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862926/suppl/GSM862926_U87-2M1-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862926/suppl/GSM862926_U87-2M1-2.CHP.gz
| | Sample_series_id | GSE35169
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862927 | GPL570 |
|
U87-2M1-3
|
U87-2M1 glioma cells derived from U87 cells subjected to an in vivo experimental lung metastasis assay and propagated in vitro
|
cell line: Invasive subline U87-2M1 derived from U87
cell type: Passage 4 cells, maintained under hypoxia
|
|
| Sample_geo_accession | GSM862927
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862927/suppl/GSM862927_U87-2M1-3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862927/suppl/GSM862927_U87-2M1-3.CHP.gz
| | Sample_series_id | GSE35169
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862928 | GPL570 |
|
2M1 - ctr1
|
U87-2M1_vector control
|
cell line: U87-2M1
cell type: Invasive subline derived from U87 glioma cells
passage: passage 4 cells
tranduced with: baculoviral control decoy vector
|
Invasive subline of U87 derived from an in vivo experimental lung metastasis assay which is subsequently propagated in vitro
|
| Sample_geo_accession | GSM862928
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin, maintained in hypoxia
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862928/suppl/GSM862928_2M1_-_ctr1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862928/suppl/GSM862928_2M1_-_ctr1.CHP.gz
| | Sample_series_id | GSE35170
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862929 | GPL570 |
|
2M1 - ctr2
|
U87-2M1_vector control
|
cell line: U87-2M1
cell type: Invasive subline derived from U87 glioma cells
passage: passage 4 cells
tranduced with: baculoviral control decoy vector
|
Invasive subline of U87 derived from an in vivo experimental lung metastasis assay which is subsequently propagated in vitro
|
| Sample_geo_accession | GSM862929
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin, maintained in hypoxia
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862929/suppl/GSM862929_2M1_-_ctr2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862929/suppl/GSM862929_2M1_-_ctr2.CHP.gz
| | Sample_series_id | GSE35170
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862930 | GPL570 |
|
2M1 - ctr3
|
U87-2M1_vector control
|
cell line: U87-2M1
cell type: Invasive subline derived from U87 glioma cells
passage: passage 4 cells
tranduced with: baculoviral control decoy vector
|
Invasive subline of U87 derived from an in vivo experimental lung metastasis assay which is subsequently propagated in vitro
|
| Sample_geo_accession | GSM862930
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin, maintained in hypoxia
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862930/suppl/GSM862930_2M1_-_ctr3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862930/suppl/GSM862930_2M1_-_ctr3.CHP.gz
| | Sample_series_id | GSE35170
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862931 | GPL570 |
|
2M1 - 10b1
|
U87-2M1_miR-10b silencing
|
cell line: U87-2M1
cell type: Invasive subline derived from U87 glioma cells
passage: passage 4 cells
tranduced with: baculoviral miR-10b decoy vector
|
Invasive subline of U87 derived from an in vivo experimental lung metastasis assay which is subsequently propagated in vitro
|
| Sample_geo_accession | GSM862931
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin, maintained in hypoxia
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862931/suppl/GSM862931_2M1_-_10b1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862931/suppl/GSM862931_2M1_-_10b1.CHP.gz
| | Sample_series_id | GSE35170
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862932 | GPL570 |
|
2M1 - 10b2
|
U87-2M1_miR-10b silencing
|
cell line: U87-2M1
cell type: Invasive subline derived from U87 glioma cells
passage: passage 4 cells
tranduced with: baculoviral miR-10b decoy vector
|
Invasive subline of U87 derived from an in vivo experimental lung metastasis assay which is subsequently propagated in vitro
|
| Sample_geo_accession | GSM862932
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin, maintained in hypoxia
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862932/suppl/GSM862932_2M1_-_10b2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862932/suppl/GSM862932_2M1_-_10b2.CHP.gz
| | Sample_series_id | GSE35170
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
GSM862933 | GPL570 |
|
2M1 - 10b3
|
U87-2M1_miR-10b silencing
|
cell line: U87-2M1
cell type: Invasive subline derived from U87 glioma cells
passage: passage 4 cells
tranduced with: baculoviral miR-10b decoy vector
|
Invasive subline of U87 derived from an in vivo experimental lung metastasis assay which is subsequently propagated in vitro
|
| Sample_geo_accession | GSM862933
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | Jan 18 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were cultured under routine conditions as described above, and detached from culture plates using trypsin, pelleted and lysed in TRIzol immediately.
| | Sample_growth_protocol_ch1 | DMEM, 10% FBS, 5% L-glutamine, 5% Penicillin-streptomycin, maintained in hypoxia
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.25 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jia Kai,,Lim
| | Sample_contact_institute | Institute of Bioengineering and Nanotechnology
| | Sample_contact_address | 31 Biopolis Way #04-01, Nanos
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138669
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862933/suppl/GSM862933_2M1_-_10b3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM862nnn/GSM862933/suppl/GSM862933_2M1_-_10b3.CHP.gz
| | Sample_series_id | GSE35170
| | Sample_series_id | GSE35208
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
