Search results for the GEO ID: GSE35230 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM864257 | GPL570 |
|
A375 1A–DMSO-1
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: DMSO
|
DMSO-1
|
Sample_geo_accession | GSM864257
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864257/suppl/GSM864257_agg-10-1723.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864258 | GPL570 |
|
A375 1B–DMSO-2
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: DMSO
|
DMSO-2
|
Sample_geo_accession | GSM864258
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864258/suppl/GSM864258_agg-10-1724.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864259 | GPL570 |
|
A375 4B–0.1 uM GSK436-2
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 0.1 uM GSK436
|
0.1 uM GSK436-2
|
Sample_geo_accession | GSM864259
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864259/suppl/GSM864259_agg-10-1726.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864260 | GPL570 |
|
A375 4C–0.1 uM GSK436-3
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 0.1 uM GSK436
|
0.1 uM GSK436-3
|
Sample_geo_accession | GSM864260
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864260/suppl/GSM864260_agg-10-1727.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864261 | GPL570 |
|
A375 5B–1 uM GSK436-2
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 1 uM GSK436
|
1 uM GSK436-2
|
Sample_geo_accession | GSM864261
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864261/suppl/GSM864261_agg-10-1729.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864262 | GPL570 |
|
A375 5C–1 uM GSK436-3
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 1 uM GSK436
|
1 uM GSK436-3
|
Sample_geo_accession | GSM864262
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864262/suppl/GSM864262_agg-10-1730.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864263 | GPL570 |
|
A375 2A–10 nM GSK212-1
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 10 nM GSK212
|
10 nM GSK212-1
|
Sample_geo_accession | GSM864263
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864263/suppl/GSM864263_agg-10-1731.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864264 | GPL570 |
|
A375 2B–10 nM GSK212-2
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 10 nM GSK212
|
10 nM GSK212-2
|
Sample_geo_accession | GSM864264
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864264/suppl/GSM864264_agg-10-1732.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864265 | GPL570 |
|
A375 2C–10 nM GSK212-3
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 10 nM GSK212
|
10 nM GSK212-3
|
Sample_geo_accession | GSM864265
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864265/suppl/GSM864265_agg-10-1733.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864266 | GPL570 |
|
A375 3B–30 nM GSK212-2
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 30 nM GSK212
|
30 nM GSK212-2
|
Sample_geo_accession | GSM864266
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864266/suppl/GSM864266_agg-10-1735.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864267 | GPL570 |
|
A375 3C–30 nM GSK212-3
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 30 nM GSK212
|
30 nM GSK212-3
|
Sample_geo_accession | GSM864267
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864267/suppl/GSM864267_agg-10-1736.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864268 | GPL570 |
|
A375 8A–10 nM GSK212 + 0.1 uM GSK436-1
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 10 nM GSK212 + 0.1 uM GSK436
|
10 nM GSK212 + 0.1 uM GSK436-1
|
Sample_geo_accession | GSM864268
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864268/suppl/GSM864268_agg-10-1737.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864269 | GPL570 |
|
A375 8B–10 nM GSK212 + 0.1 uM GSK436-2
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 10 nM GSK212 + 0.1 uM GSK436
|
10 nM GSK212 + 0.1 uM GSK436-2
|
Sample_geo_accession | GSM864269
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864269/suppl/GSM864269_agg-10-1738.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864270 | GPL570 |
|
A375 8C–10 nM GSK212 + 0.1 uM GSK436-3
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 10 nM GSK212 + 0.1 uM GSK436
|
10 nM GSK212 + 0.1 uM GSK436-3
|
Sample_geo_accession | GSM864270
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864270/suppl/GSM864270_agg-10-1739.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864271 | GPL570 |
|
A375 9A–10 nM GSK212 + 1 uM GSK436-1
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 10 nM GSK212 + 1 uM GSK436
|
10 nM GSK212 + 1 uM GSK436-1
|
Sample_geo_accession | GSM864271
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864271/suppl/GSM864271_agg-10-1740.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864272 | GPL570 |
|
A375 9B–10 nM GSK212 + 1 uM GSK436-2
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 10 nM GSK212 + 1 uM GSK436
|
10 nM GSK212 + 1 uM GSK436-2
|
Sample_geo_accession | GSM864272
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864272/suppl/GSM864272_agg-10-1741.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864273 | GPL570 |
|
A375 9C–10 nM GSK212 + 1 uM GSK436-3
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: 10 nM GSK212 + 1 uM GSK436
|
10 nM GSK212 + 1 uM GSK436-3
|
Sample_geo_accession | GSM864273
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864273/suppl/GSM864273_agg-10-1742.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864274 | GPL570 |
|
A375 16R6-4 1A–DMSO-1
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: DMSO
|
DMSO-1
|
Sample_geo_accession | GSM864274
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864274/suppl/GSM864274_agg-10-1743.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864275 | GPL570 |
|
A375 16R6-4 1B–DMSO-2
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: DMSO
|
DMSO-2
|
Sample_geo_accession | GSM864275
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864275/suppl/GSM864275_agg-10-1744.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864276 | GPL570 |
|
A375 16R6-4 4A–0.1 uM GSK436-1
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 0.1 uM GSK436
|
0.1 uM GSK436-1
|
Sample_geo_accession | GSM864276
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864276/suppl/GSM864276_agg-10-1745.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864277 | GPL570 |
|
A375 16R6-4 4B–0.1 uM GSK436-2
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 0.1 uM GSK436
|
0.1 uM GSK436-2
|
Sample_geo_accession | GSM864277
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864277/suppl/GSM864277_agg-10-1746.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864278 | GPL570 |
|
A375 16R6-4 4C–0.1 uM GSK436-3
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 0.1 uM GSK436
|
0.1 uM GSK436-3
|
Sample_geo_accession | GSM864278
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864278/suppl/GSM864278_agg-10-1747.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864279 | GPL570 |
|
A375 16R6-4 5A–1 uM GSK436-1
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 1 uM GSK436
|
1 uM GSK436-1
|
Sample_geo_accession | GSM864279
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864279/suppl/GSM864279_agg-10-1748.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864280 | GPL570 |
|
A375 16R6-4 5B–1 uM GSK436-2
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 1 uM GSK436
|
1 uM GSK436-2
|
Sample_geo_accession | GSM864280
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864280/suppl/GSM864280_agg-10-1749.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864281 | GPL570 |
|
A375 16R6-4 5C–1 uM GSK436-3
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 1 uM GSK436
|
1 uM GSK436-3
|
Sample_geo_accession | GSM864281
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864281/suppl/GSM864281_agg-10-1750.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864282 | GPL570 |
|
A375 16R6-4 2A–10 nM GSK212-1
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 10 nM GSK212
|
10 nM GSK212-1
|
Sample_geo_accession | GSM864282
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864282/suppl/GSM864282_agg-10-1751.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864283 | GPL570 |
|
A375 16R6-4 2B–10 nM GSK212-2
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 10 nM GSK212
|
10 nM GSK212-2
|
Sample_geo_accession | GSM864283
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864283/suppl/GSM864283_agg-10-1752.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864284 | GPL570 |
|
A375 16R6-4 2C–10 nM GSK212-3
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 10 nM GSK212
|
10 nM GSK212-3
|
Sample_geo_accession | GSM864284
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864284/suppl/GSM864284_agg-10-1753.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864285 | GPL570 |
|
A375 16R6-4 3A–30 nM GSK212-1
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 30 nM GSK212
|
30 nM GSK212-1
|
Sample_geo_accession | GSM864285
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864285/suppl/GSM864285_agg-10-1754.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864286 | GPL570 |
|
A375 16R6-4 3B–30 nM GSK212-2
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 30 nM GSK212
|
30 nM GSK212-2
|
Sample_geo_accession | GSM864286
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864286/suppl/GSM864286_agg-10-1755.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864287 | GPL570 |
|
A375 16R6-4 3C–30 nM GSK212-3
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 30 nM GSK212
|
30 nM GSK212-3
|
Sample_geo_accession | GSM864287
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864287/suppl/GSM864287_agg-10-1756.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864288 | GPL570 |
|
A375 16R6-4 8A–10 nM GSK212 + 0.1 uM GSK436-1
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 10 nM GSK212 + 0.1 uM GSK436
|
10 nM GSK212 + 0.1 uM GSK436-1
|
Sample_geo_accession | GSM864288
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864288/suppl/GSM864288_agg-10-1757.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864289 | GPL570 |
|
A375 16R6-4 8B–10 nM GSK212 + 0.1 uM GSK436-2
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 10 nM GSK212 + 0.1 uM GSK436
|
10 nM GSK212 + 0.1 uM GSK436-2
|
Sample_geo_accession | GSM864289
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864289/suppl/GSM864289_agg-10-1758.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864290 | GPL570 |
|
A375 16R6-4 8C–10 nM GSK212 + 0.1 uM GSK436-3
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 10 nM GSK212 + 0.1 uM GSK436
|
10 nM GSK212 + 0.1 uM GSK436-3
|
Sample_geo_accession | GSM864290
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864290/suppl/GSM864290_agg-10-1759.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864291 | GPL570 |
|
A375 16R6-4 9A–10 nM GSK212 + 1 uM GSK436-1
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 10 nM GSK212 + 1 uM GSK436
|
10 nM GSK212 + 1 uM GSK436-1
|
Sample_geo_accession | GSM864291
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864291/suppl/GSM864291_agg-10-1760.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864292 | GPL570 |
|
A375 16R6-4 9B–10 nM GSK212 + 1 uM GSK436-2
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 10 nM GSK212 + 1 uM GSK436
|
10 nM GSK212 + 1 uM GSK436-2
|
Sample_geo_accession | GSM864292
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864292/suppl/GSM864292_agg-10-1761.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864293 | GPL570 |
|
A375 16R6-4 9C–10 nM GSK212 + 1 uM GSK436-3
|
A375 Melanoma cell line
|
cell type: GSK436 resistant clone
cell line: A375
treatment: 10 nM GSK212 + 1 uM GSK436
|
10 nM GSK212 + 1 uM GSK436-3
|
Sample_geo_accession | GSM864293
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864293/suppl/GSM864293_agg-10-1762.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
GSM864294 | GPL570 |
|
A375 1C–DMSO-3
|
A375 Melanoma cell line
|
cell type: Parental cells
cell line: A375
treatment: DMSO
|
DMSO-3
|
Sample_geo_accession | GSM864294
| Sample_status | Public on Feb 01 2012
| Sample_submission_date | Jan 20 2012
| Sample_last_update_date | Feb 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with compounds for 24hrs at 37°C
| Sample_growth_protocol_ch1 | Grow A375 and A375 16R6-4 cells in RPMI1640 supplemented with 10% Fetal Bovin Serum
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng of total RNA
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| Sample_data_processing | The data was normalized in Microarray Suite version 5.0 (MAS 5.0) with the trimmed mean target intensity of each array arbitrarily set to 150. Further analysis was conducted with Array Studio V5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Vivian,,Zhang
| Sample_contact_email | vivian.z.zhang@gsk.com
| Sample_contact_phone | (610) 917-6324
| Sample_contact_department | Oncology Translational Research
| Sample_contact_institute | GlaxoSmithKline
| Sample_contact_address | 1250 S. Collegeville Rd
| Sample_contact_city | Collegeville
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19426
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM864nnn/GSM864294/suppl/GSM864294_agg-10-1777.CEL.gz
| Sample_series_id | GSE35230
| Sample_data_row_count | 54675
| |
|
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