Result

Search results for the GEO ID: GSE35293

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM865266

GPL339

WT littermate 1

enterocytes isolated from mouse small intestine

genotype/variation: Wildtype gender: male tissue: small intestine age: 2.5-3.0 month strain: C57BL/6

Sample_geo_accession	GSM865266
Sample_status	Public on Apr 11 2012
Sample_submission_date	Jan 24 2012
Sample_last_update_date	Apr 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were sacrificed by CO. The small intestine was dissected and enterocytes were isolated following the procedure of Zhang et al., Drug Metab. Dispos., 31:1346-1351, 2003.
Sample_growth_protocol_ch1	Animals were maintained at 22°C with a 12-h light on, 12-h light off cycle and were allowed free access to water and a standard laboratory diet.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared from enterocytes using TRIzol and the samples were further purified using Rneasy mini columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 micrograms of total RNA were used for synthesis of biotinylated antisense RNA with MESSAGEAMP aRNA kit from Ambion.
Sample_hyb_protocol	Following fragmentation, 10 ug of aRNA were hybridized for 16 hr at 45°C in a GeneChip hybrization oven with constant rotation. Arrays were then stained and washed using the antibody and staining protocol from Affymetrix in a GeneChip Fluidics Station 400.
Sample_scan_protocol	The expression data were collected with a gene array scanner using Affymetrix Microarray Suite Version 5.0 (MAS 5.0)
Sample_data_processing	The experimental data sets were normalized using GCRMA program in GeneSpring 10.0. The data from the WT littermates was used as the baseline. Probe sets with raw intensity below the 20th percentile in all eight samples were eliminated. Analysis for signifigance was performed using the unpaired t-test.
Sample_platform_id	GPL339
Sample_contact_name	Jaime,,D'Agostino
Sample_contact_email	jaimedag@umich.edu
Sample_contact_institute	University of Michigan
Sample_contact_address	2025 S huron Parkway apt 102
Sample_contact_city	Ann Arbor
Sample_contact_state	MI
Sample_contact_zip/postal_code	48104
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865266/suppl/GSM865266_WT_littermate_1.CEL.gz
Sample_series_id	GSE35293
Sample_data_row_count	18443

GSM865267

GPL339

WT littermate 2

enterocytes isolated from mouse small intestine

genotype/variation: Wildtype gender: male tissue: small intestine age: 2.5-3.0 month strain: C57BL/6

Sample_geo_accession	GSM865267
Sample_status	Public on Apr 11 2012
Sample_submission_date	Jan 24 2012
Sample_last_update_date	Apr 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were sacrificed by CO. The small intestine was dissected and enterocytes were isolated following the procedure of Zhang et al., Drug Metab. Dispos., 31:1346-1351, 2003.
Sample_growth_protocol_ch1	Animals were maintained at 22°C with a 12-h light on, 12-h light off cycle and were allowed free access to water and a standard laboratory diet.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared from enterocytes using TRIzol and the samples were further purified using Rneasy mini columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 micrograms of total RNA were used for synthesis of biotinylated antisense RNA with MESSAGEAMP aRNA kit from Ambion.
Sample_hyb_protocol	Following fragmentation, 10 ug of aRNA were hybridized for 16 hr at 45°C in a GeneChip hybrization oven with constant rotation. Arrays were then stained and washed using the antibody and staining protocol from Affymetrix in a GeneChip Fluidics Station 400.
Sample_scan_protocol	The expression data were collected with a gene array scanner using Affymetrix Microarray Suite Version 5.0 (MAS 5.0)
Sample_data_processing	The experimental data sets were normalized using GCRMA program in GeneSpring 10.0. The data from the WT littermates was used as the baseline. Probe sets with raw intensity below the 20th percentile in all eight samples were eliminated. Analysis for signifigance was performed using the unpaired t-test.
Sample_platform_id	GPL339
Sample_contact_name	Jaime,,D'Agostino
Sample_contact_email	jaimedag@umich.edu
Sample_contact_institute	University of Michigan
Sample_contact_address	2025 S huron Parkway apt 102
Sample_contact_city	Ann Arbor
Sample_contact_state	MI
Sample_contact_zip/postal_code	48104
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865267/suppl/GSM865267_Wt_littermate_2.CEL.gz
Sample_series_id	GSE35293
Sample_data_row_count	18443

GSM865268

GPL339

WT littermate 3

enterocytes isolated from mouse small intestine

genotype/variation: Wildtype gender: male tissue: small intestine age: 2.5-3.0 month strain: C57BL/6

Sample_geo_accession	GSM865268
Sample_status	Public on Apr 11 2012
Sample_submission_date	Jan 24 2012
Sample_last_update_date	Apr 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were sacrificed by CO. The small intestine was dissected and enterocytes were isolated following the procedure of Zhang et al., Drug Metab. Dispos., 31:1346-1351, 2003.
Sample_growth_protocol_ch1	Animals were maintained at 22°C with a 12-h light on, 12-h light off cycle and were allowed free access to water and a standard laboratory diet.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared from enterocytes using TRIzol and the samples were further purified using Rneasy mini columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 micrograms of total RNA were used for synthesis of biotinylated antisense RNA with MESSAGEAMP aRNA kit from Ambion.
Sample_hyb_protocol	Following fragmentation, 10 ug of aRNA were hybridized for 16 hr at 45°C in a GeneChip hybrization oven with constant rotation. Arrays were then stained and washed using the antibody and staining protocol from Affymetrix in a GeneChip Fluidics Station 400.
Sample_scan_protocol	The expression data were collected with a gene array scanner using Affymetrix Microarray Suite Version 5.0 (MAS 5.0)
Sample_data_processing	The experimental data sets were normalized using GCRMA program in GeneSpring 10.0. The data from the WT littermates was used as the baseline. Probe sets with raw intensity below the 20th percentile in all eight samples were eliminated. Analysis for signifigance was performed using the unpaired t-test.
Sample_platform_id	GPL339
Sample_contact_name	Jaime,,D'Agostino
Sample_contact_email	jaimedag@umich.edu
Sample_contact_institute	University of Michigan
Sample_contact_address	2025 S huron Parkway apt 102
Sample_contact_city	Ann Arbor
Sample_contact_state	MI
Sample_contact_zip/postal_code	48104
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865268/suppl/GSM865268_WT_littermate_3.CEL.gz
Sample_series_id	GSE35293
Sample_data_row_count	18443

GSM865269

GPL339

WT littermate 4

enterocytes isolated from mouse small intestine

genotype/variation: Wildtype gender: male tissue: small intestine age: 2.5-3.0 month strain: C57BL/6

Sample_geo_accession	GSM865269
Sample_status	Public on Apr 11 2012
Sample_submission_date	Jan 24 2012
Sample_last_update_date	Apr 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were sacrificed by CO. The small intestine was dissected and enterocytes were isolated following the procedure of Zhang et al., Drug Metab. Dispos., 31:1346-1351, 2003.
Sample_growth_protocol_ch1	Animals were maintained at 22°C with a 12-h light on, 12-h light off cycle and were allowed free access to water and a standard laboratory diet.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared from enterocytes using TRIzol and the samples were further purified using Rneasy mini columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 micrograms of total RNA were used for synthesis of biotinylated antisense RNA with MESSAGEAMP aRNA kit from Ambion.
Sample_hyb_protocol	Following fragmentation, 10 ug of aRNA were hybridized for 16 hr at 45°C in a GeneChip hybrization oven with constant rotation. Arrays were then stained and washed using the antibody and staining protocol from Affymetrix in a GeneChip Fluidics Station 400.
Sample_scan_protocol	The expression data were collected with a gene array scanner using Affymetrix Microarray Suite Version 5.0 (MAS 5.0)
Sample_data_processing	The experimental data sets were normalized using GCRMA program in GeneSpring 10.0. The data from the WT littermates was used as the baseline. Probe sets with raw intensity below the 20th percentile in all eight samples were eliminated. Analysis for signifigance was performed using the unpaired t-test.
Sample_platform_id	GPL339
Sample_contact_name	Jaime,,D'Agostino
Sample_contact_email	jaimedag@umich.edu
Sample_contact_institute	University of Michigan
Sample_contact_address	2025 S huron Parkway apt 102
Sample_contact_city	Ann Arbor
Sample_contact_state	MI
Sample_contact_zip/postal_code	48104
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865269/suppl/GSM865269_WT_littermate_4.CEL.gz
Sample_series_id	GSE35293
Sample_data_row_count	18443

GSM865270

GPL339

IE-Cpr-Null 1

enterocytes isolated from mouse small intestine

genotype/variation: IE-Cpr-Null gender: male tissue: small intestine age: 2.5-3.0 month strain: C57BL/6

Sample_geo_accession	GSM865270
Sample_status	Public on Apr 11 2012
Sample_submission_date	Jan 24 2012
Sample_last_update_date	Apr 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were sacrificed by CO. The small intestine was dissected and enterocytes were isolated following the procedure of Zhang et al., Drug Metab. Dispos., 31:1346-1351, 2003.
Sample_growth_protocol_ch1	Animals were maintained at 22°C with a 12-h light on, 12-h light off cycle and were allowed free access to water and a standard laboratory diet.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared from enterocytes using TRIzol and the samples were further purified using Rneasy mini columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 micrograms of total RNA were used for synthesis of biotinylated antisense RNA with MESSAGEAMP aRNA kit from Ambion.
Sample_hyb_protocol	Following fragmentation, 10 ug of aRNA were hybridized for 16 hr at 45°C in a GeneChip hybrization oven with constant rotation. Arrays were then stained and washed using the antibody and staining protocol from Affymetrix in a GeneChip Fluidics Station 400.
Sample_scan_protocol	The expression data were collected with a gene array scanner using Affymetrix Microarray Suite Version 5.0 (MAS 5.0)
Sample_data_processing	The experimental data sets were normalized using GCRMA program in GeneSpring 10.0. The data from the WT littermates was used as the baseline. Probe sets with raw intensity below the 20th percentile in all eight samples were eliminated. Analysis for signifigance was performed using the unpaired t-test.
Sample_platform_id	GPL339
Sample_contact_name	Jaime,,D'Agostino
Sample_contact_email	jaimedag@umich.edu
Sample_contact_institute	University of Michigan
Sample_contact_address	2025 S huron Parkway apt 102
Sample_contact_city	Ann Arbor
Sample_contact_state	MI
Sample_contact_zip/postal_code	48104
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865270/suppl/GSM865270_IE-Cpr-null_1.CEL.gz
Sample_series_id	GSE35293
Sample_data_row_count	18443

GSM865271

GPL339

IE-Cpr-Null 2

enterocytes isolated from mouse small intestine

genotype/variation: IE-Cpr-Null gender: male tissue: small intestine age: 2.5-3.0 month strain: C57BL/6

Sample_geo_accession	GSM865271
Sample_status	Public on Apr 11 2012
Sample_submission_date	Jan 24 2012
Sample_last_update_date	Apr 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were sacrificed by CO. The small intestine was dissected and enterocytes were isolated following the procedure of Zhang et al., Drug Metab. Dispos., 31:1346-1351, 2003.
Sample_growth_protocol_ch1	Animals were maintained at 22°C with a 12-h light on, 12-h light off cycle and were allowed free access to water and a standard laboratory diet.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared from enterocytes using TRIzol and the samples were further purified using Rneasy mini columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 micrograms of total RNA were used for synthesis of biotinylated antisense RNA with MESSAGEAMP aRNA kit from Ambion.
Sample_hyb_protocol	Following fragmentation, 10 ug of aRNA were hybridized for 16 hr at 45°C in a GeneChip hybrization oven with constant rotation. Arrays were then stained and washed using the antibody and staining protocol from Affymetrix in a GeneChip Fluidics Station 400.
Sample_scan_protocol	The expression data were collected with a gene array scanner using Affymetrix Microarray Suite Version 5.0 (MAS 5.0)
Sample_data_processing	The experimental data sets were normalized using GCRMA program in GeneSpring 10.0. The data from the WT littermates was used as the baseline. Probe sets with raw intensity below the 20th percentile in all eight samples were eliminated. Analysis for signifigance was performed using the unpaired t-test.
Sample_platform_id	GPL339
Sample_contact_name	Jaime,,D'Agostino
Sample_contact_email	jaimedag@umich.edu
Sample_contact_institute	University of Michigan
Sample_contact_address	2025 S huron Parkway apt 102
Sample_contact_city	Ann Arbor
Sample_contact_state	MI
Sample_contact_zip/postal_code	48104
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865271/suppl/GSM865271_IE-Cpr-null_2.CEL.gz
Sample_series_id	GSE35293
Sample_data_row_count	18443

GSM865272

GPL339

IE-Cpr-Null 3

enterocytes isolated from mouse small intestine

genotype/variation: IE-Cpr-Null gender: male tissue: small intestine age: 2.5-3.0 month strain: C57BL/6

Sample_geo_accession	GSM865272
Sample_status	Public on Apr 11 2012
Sample_submission_date	Jan 24 2012
Sample_last_update_date	Apr 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were sacrificed by CO. The small intestine was dissected and enterocytes were isolated following the procedure of Zhang et al., Drug Metab. Dispos., 31:1346-1351, 2003.
Sample_growth_protocol_ch1	Animals were maintained at 22°C with a 12-h light on, 12-h light off cycle and were allowed free access to water and a standard laboratory diet.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared from enterocytes using TRIzol and the samples were further purified using Rneasy mini columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 micrograms of total RNA were used for synthesis of biotinylated antisense RNA with MESSAGEAMP aRNA kit from Ambion.
Sample_hyb_protocol	Following fragmentation, 10 ug of aRNA were hybridized for 16 hr at 45°C in a GeneChip hybrization oven with constant rotation. Arrays were then stained and washed using the antibody and staining protocol from Affymetrix in a GeneChip Fluidics Station 400.
Sample_scan_protocol	The expression data were collected with a gene array scanner using Affymetrix Microarray Suite Version 5.0 (MAS 5.0)
Sample_data_processing	The experimental data sets were normalized using GCRMA program in GeneSpring 10.0. The data from the WT littermates was used as the baseline. Probe sets with raw intensity below the 20th percentile in all eight samples were eliminated. Analysis for signifigance was performed using the unpaired t-test.
Sample_platform_id	GPL339
Sample_contact_name	Jaime,,D'Agostino
Sample_contact_email	jaimedag@umich.edu
Sample_contact_institute	University of Michigan
Sample_contact_address	2025 S huron Parkway apt 102
Sample_contact_city	Ann Arbor
Sample_contact_state	MI
Sample_contact_zip/postal_code	48104
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865272/suppl/GSM865272_IE-Cpr-null_3.CEL.gz
Sample_series_id	GSE35293
Sample_data_row_count	18443

GSM865273

GPL339

IE-Cpr-Null 4

enterocytes isolated from mouse small intestine

genotype/variation: IE-Cpr-Null gender: male tissue: small intestine age: 2.5-3.0 month strain: C57BL/6

Sample_geo_accession	GSM865273
Sample_status	Public on Apr 11 2012
Sample_submission_date	Jan 24 2012
Sample_last_update_date	Apr 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were sacrificed by CO. The small intestine was dissected and enterocytes were isolated following the procedure of Zhang et al., Drug Metab. Dispos., 31:1346-1351, 2003.
Sample_growth_protocol_ch1	Animals were maintained at 22°C with a 12-h light on, 12-h light off cycle and were allowed free access to water and a standard laboratory diet.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared from enterocytes using TRIzol and the samples were further purified using Rneasy mini columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 micrograms of total RNA were used for synthesis of biotinylated antisense RNA with MESSAGEAMP aRNA kit from Ambion.
Sample_hyb_protocol	Following fragmentation, 10 ug of aRNA were hybridized for 16 hr at 45°C in a GeneChip hybrization oven with constant rotation. Arrays were then stained and washed using the antibody and staining protocol from Affymetrix in a GeneChip Fluidics Station 400.
Sample_scan_protocol	The expression data were collected with a gene array scanner using Affymetrix Microarray Suite Version 5.0 (MAS 5.0)
Sample_data_processing	The experimental data sets were normalized using GCRMA program in GeneSpring 10.0. The data from the WT littermates was used as the baseline. Probe sets with raw intensity below the 20th percentile in all eight samples were eliminated. Analysis for signifigance was performed using the unpaired t-test.
Sample_platform_id	GPL339
Sample_contact_name	Jaime,,D'Agostino
Sample_contact_email	jaimedag@umich.edu
Sample_contact_institute	University of Michigan
Sample_contact_address	2025 S huron Parkway apt 102
Sample_contact_city	Ann Arbor
Sample_contact_state	MI
Sample_contact_zip/postal_code	48104
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865273/suppl/GSM865273_IE-Cpr-null_4.CEL.gz
Sample_series_id	GSE35293
Sample_data_row_count	18443

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