Search results for the GEO ID: GSE35318  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM865722 | GPL1261 |
|
KO TLR2L 0h-A
|
LN DCs unstimulated
|
strain: C57BL/6
genotype/variation: p38a-knockout
|
Gene expression data from p38a-KO DCs without TLR2 activation
|
| Sample_geo_accession | GSM865722
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865722/suppl/GSM865722.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865723 | GPL1261 |
|
KO TLR2L 0h-B
|
LN DCs unstimulated
|
strain: C57BL/6
genotype/variation: p38a-knockout
|
Gene expression data from p38a-KO DCs without TLR2 activation
|
| Sample_geo_accession | GSM865723
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865723/suppl/GSM865723.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865724 | GPL1261 |
|
KO TLR2L 2h-A
|
LN DCs TLR2-stimulated, 2 h
|
strain: C57BL/6
genotype/variation: p38a-knockout
|
Gene expression data from p38a-KO DCs 2 h after TLR2 activation
|
| Sample_geo_accession | GSM865724
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865724/suppl/GSM865724.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865725 | GPL1261 |
|
KO TLR2L 2h-B
|
LN DCs TLR2-stimulated, 2 h
|
strain: C57BL/6
genotype/variation: p38a-knockout
|
Gene expression data from p38a-KO DCs 2 h after TLR2 activation
|
| Sample_geo_accession | GSM865725
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865725/suppl/GSM865725.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865726 | GPL1261 |
|
KO TLR2L 4h-A
|
LN DCs TLR2-stimulated, 4 h
|
strain: C57BL/6
genotype/variation: p38a-knockout
|
Gene expression data from p38a-KO DCs 4 h after TLR2 activation
|
| Sample_geo_accession | GSM865726
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865726/suppl/GSM865726.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865727 | GPL1261 |
|
KO TLR2L 4h-B
|
LN DCs TLR2-stimulated, 4 h
|
strain: C57BL/6
genotype/variation: p38a-knockout
|
Gene expression data from p38a-KO DCs 4 h after TLR2 activation
|
| Sample_geo_accession | GSM865727
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865727/suppl/GSM865727.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865728 | GPL1261 |
|
WT TLR2L 0h-A
|
LN DCs unstimulated
|
strain: C57BL/6
genotype/variation: wild type
|
Gene expression data from WT DCs without TLR2 activation
|
| Sample_geo_accession | GSM865728
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865728/suppl/GSM865728.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865729 | GPL1261 |
|
WT TLR2L 0h-B
|
LN DCs unstimulated
|
strain: C57BL/6
genotype/variation: wild type
|
Gene expression data from WT DCs without TLR2 activation
|
| Sample_geo_accession | GSM865729
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865729/suppl/GSM865729.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865730 | GPL1261 |
|
WT TLR2L 2h-A
|
LN DCs TLR2-stimulated, 2 h
|
strain: C57BL/6
genotype/variation: wild type
|
Gene expression data from WT DCs 2 h after TLR2 activation
|
| Sample_geo_accession | GSM865730
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865730/suppl/GSM865730.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865731 | GPL1261 |
|
WT TLR2L 2h-B
|
LN DCs TLR2-stimulated, 2 h
|
strain: C57BL/6
genotype/variation: wild type
|
Gene expression data from WT DCs 2 h after TLR2 activation
|
| Sample_geo_accession | GSM865731
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865731/suppl/GSM865731.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865732 | GPL1261 |
|
WT TLR2L 4h-A
|
LN DCs TLR2-stimulated, 4 h
|
strain: C57BL/6
genotype/variation: wild type
|
Gene expression data from WT DCs 4 h after TLR2 activation
|
| Sample_geo_accession | GSM865732
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865732/suppl/GSM865732.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
GSM865733 | GPL1261 |
|
WT TLR2L 4h-B
|
LN DCs TLR2-stimulated, 4 h
|
strain: C57BL/6
genotype/variation: wild type
|
Gene expression data from WT DCs 4 h after TLR2 activation
|
| Sample_geo_accession | GSM865733
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Jan 24 2012
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pam3CSK4 (1 mg/ml) was added to DC culture. DCs were incubated at 37C for 0, 2, and 4 h after treatment.
| | Sample_growth_protocol_ch1 | Mouse primary dendritic cells were isolated from lymph nodes and cultured at 37C until treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by using Trizol (Invitrogen) and then purified by using RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | GeneChip 3' IVT Express Labeling Kit was used to prepare biotinylated cRNA according to the standard Affymetrix protocol from 0.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. Arrays were washed and stained by streptavidin-phycoerythrin conjugate in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jin Mo ,,Park
| | Sample_contact_email | jmpark@cbrc2.mgh.harvard.edu
| | Sample_contact_phone | 617-643-2328
| | Sample_contact_fax | 617-726-4453
| | Sample_contact_department | Cutaneous Biology Research Center
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 149 Thirteenth Street
| | Sample_contact_city | Charlestown
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02129
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM865nnn/GSM865733/suppl/GSM865733.CEL.gz
| | Sample_series_id | GSE35318
| | Sample_data_row_count | 45101
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
