Search results for the GEO ID: GSE35340  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM866353 | GPL570 |
|
LC_1
|
Skin- epidermal Langerhans cells
|
tissue origin: skin
dendritic cell lineages: epidermal Langerhans cells
cell type: sorted CD1a+, epidermal Langerhans cells
|
LC1
|
| Sample_geo_accession | GSM866353
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866353/suppl/GSM866353_p0748_CH13.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866354 | GPL570 |
|
LC_2
|
Skin- epidermal Langerhans cells
|
tissue origin: skin
dendritic cell lineages: epidermal Langerhans cells
cell type: CD1a+, epidermal Langerhans cells
|
LC2
|
| Sample_geo_accession | GSM866354
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866354/suppl/GSM866354_p0813_4.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866355 | GPL570 |
|
LC_3
|
Skin- epidermal Langerhans cells
|
tissue origin: skin
dendritic cell lineages: epidermal Langerhans cells
cell type: CD1a+, epidermal Langerhans cells
|
LC3
|
| Sample_geo_accession | GSM866355
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866355/suppl/GSM866355_p0813_5.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866356 | GPL570 |
|
LCH_1
|
Langerhans cell histiocytosis lesion-bone
|
tissue origin: bone
cell type: sorted CD207+, CD1a+ LCH cells
|
LCH1
|
| Sample_geo_accession | GSM866356
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866356/suppl/GSM866356_p0748_CH10.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866357 | GPL570 |
|
LCH_2
|
Langerhans cell histiocytosis lesion-bone
|
tissue origin: bone
cell type: sorted CD207+, CD1a+ LCH cells
|
LCH2
|
| Sample_geo_accession | GSM866357
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866357/suppl/GSM866357_p0748_CH12.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866358 | GPL570 |
|
LCH_3
|
Langerhans cell histiocytosis lesion-bone
|
tissue origin: bone
cell type: sorted CD207+, CD1a+ LCH cells
|
LCH3
|
| Sample_geo_accession | GSM866358
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866358/suppl/GSM866358_p0748_CH9.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866359 | GPL570 |
|
LCH_4
|
Langerhans cell histiocytosis lesion-bone
|
tissue origin: bone
cell type: sorted CD207+, CD1a+ LCH cells
|
LCH4
|
| Sample_geo_accession | GSM866359
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866359/suppl/GSM866359_p0813_1.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866360 | GPL570 |
|
LCH_5
|
Langerhans cell histiocytosis lesion-bone
|
tissue origin: bone
cell type: sorted CD207+, CD1a+ LCH cells
|
LCH5
|
| Sample_geo_accession | GSM866360
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866360/suppl/GSM866360_p0813_5nov.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866361 | GPL570 |
|
LCH_6
|
Langerhans cell histiocytosis lesion-skin
|
tissue origin: skin
cell type: sorted CD207+, CD1a+ LCH cells
|
LCH6
|
| Sample_geo_accession | GSM866361
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866361/suppl/GSM866361_1.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866362 | GPL570 |
|
LCH_7
|
Langerhans cell histiocytosis lesion-bone
|
tissue origin: bone
cell type: sorted CD207+, CD1a+ LCH cells
|
LCH7
|
| Sample_geo_accession | GSM866362
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866362/suppl/GSM866362_2.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866363 | GPL570 |
|
LCH_8
|
Langerhans cell histiocytosis lesion-mucosa
|
tissue origin: mucosa
cell type: sorted CD207+, CD1a+ LCH cells
|
LCH8
|
| Sample_geo_accession | GSM866363
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866363/suppl/GSM866363_6.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866364 | GPL570 |
|
mDC_1
|
peripheral blood- sorted myeloid dendritic cell (mDC1)
|
tissue origin: peripheral blood
dendritic cell lineages: myeloid dendritic cells (mDC1)
cell type: sorted HLA-DR+/CD45RA hi/CD11c-/BDCA2+ cells
|
mDC1
|
| Sample_geo_accession | GSM866364
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866364/suppl/GSM866364_8.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866365 | GPL570 |
|
mDC_2
|
peripheral blood- sorted myeloid dendritic cell (mDC1)
|
tissue origin: peripheral blood
dendritic cell lineages: myeloid dendritic cells (mDC1)
cell type: sorted HLA-DR+/CD45RA hi/CD11c-/BDCA2+ cells
|
mDC2
|
| Sample_geo_accession | GSM866365
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866365/suppl/GSM866365_9.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866366 | GPL570 |
|
mDC_3
|
peripheral blood- sorted myeloid dendritic cell (mDC1)
|
tissue origin: peripheral blood
dendritic cell lineages: myeloid dendritic cells (mDC1)
cell type: sorted HLA-DR+/CD45RA hi/CD11c-/BDCA2+ cells
|
mDC3
|
| Sample_geo_accession | GSM866366
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866366/suppl/GSM866366_10.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866367 | GPL570 |
|
pdC_1
|
peripheral blood-sorted plasmacytoid dendritic cell (pdC)
|
tissue origin: peripheral blood
dendritic cell lineages: plasmacytoid dendritic cells (pDC)
cell type: sorted HLA-DR+/CD11c+/BDCA1+/BDCA3- cells
|
pdC1
|
| Sample_geo_accession | GSM866367
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866367/suppl/GSM866367_3.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866368 | GPL570 |
|
pdC_2
|
peripheral blood-sorted plasmacytoid dendritic cell (pdC)
|
tissue origin: peripheral blood
dendritic cell lineages: plasmacytoid dendritic cells (pDC)
cell type: sorted HLA-DR+/CD11c+/BDCA1+/BDCA3- cells
|
pdC2
|
| Sample_geo_accession | GSM866368
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866368/suppl/GSM866368_4.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
|
GSM866369 | GPL570 |
|
pdC_3
|
peripheral blood-sorted plasmacytoid dendritic cell (pdC)
|
tissue origin: peripheral blood
dendritic cell lineages: plasmacytoid dendritic cells (pDC)
cell type: sorted HLA-DR+/CD11c+/BDCA1+/BDCA3- cells
|
pdC3
|
| Sample_geo_accession | GSM866369
| | Sample_status | Public on Oct 24 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Oct 24 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extrated usingTrizol according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | The preparation of labeled cRNA was performed according to the the GeneChip® Expression 3'-Amplification Reagents Two-cycle cDNA synthesis kit instructions (Affymetrix Inc, Santa Clara, CA, USA) to produce double-stranded cDNA.
| | Sample_hyb_protocol | Resulting double-stranded cDNA was used as a template to generate biotin-targeted cRNA following manufacturer’s specifications. Fifteen micrograms of the biotin labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 micrograms of which was hybridised onto the GeneChip® Human Genome U133 Plus 2.0 Array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures. The arrays were washed and then stained in a GeneChip® Fluidics Station 45.
| | Sample_scan_protocol | Scanning was carried out with the GeneChip® Scanner 3000 and image analysis was performed using GeneChip® Operating Software.
| | Sample_data_processing | The data was analyzed in R statistical environment using Bioconductor packages. Normalization was done using the gcRMA algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Maximilian,,Kauer
| | Sample_contact_email | maximilian.kauer@ccri.at
| | Sample_contact_laboratory | Heinrich Kovar
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | CCRI, St.Anna Children Cancer Research Institute
| | Sample_contact_address | Zimmermannplatz 10
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1090
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866369/suppl/GSM866369_5.CEL.gz
| | Sample_series_id | GSE35340
| | Sample_data_row_count | 54675
| | |
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