Search results for the GEO ID: GSE35357 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM866738 | GPL1261 |
|
control tongue at E13.5, biological rep1
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/+
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
|
Sample_geo_accession | GSM866738
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866738/suppl/GSM866738_Control_1.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866739 | GPL1261 |
|
control tongue at E13.5, biological rep2
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/+
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
|
Sample_geo_accession | GSM866739
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866739/suppl/GSM866739_Control_2.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866740 | GPL1261 |
|
control tongue at E13.5, biological rep3
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/+
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866740
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866740/suppl/GSM866740_Control_3.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866741 | GPL1261 |
|
control tongue at E13.5, biological rep4
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/+
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866741
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866741/suppl/GSM866741_Control_4.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866742 | GPL1261 |
|
control tongue at E13.5, biological rep5
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/+
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866742
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866742/suppl/GSM866742_Control_5.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866743 | GPL1261 |
|
control tongue at E13.5, biological rep6
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/+
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866743
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866743/suppl/GSM866743_Control_6.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866744 | GPL1261 |
|
mutant tongue at E13.5, biological rep1
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/flox
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866744
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866744/suppl/GSM866744_Mutant_1.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866745 | GPL1261 |
|
mutant tongue at E13.5, biological rep2
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/flox
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866745
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866745/suppl/GSM866745_Mutant_2.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866746 | GPL1261 |
|
mutant tongue at E13.5, biological rep3
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/flox
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866746
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866746/suppl/GSM866746_Mutant_3.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866747 | GPL1261 |
|
mutant tongue at E13.5, biological rep4
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/flox
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866747
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866747/suppl/GSM866747_Mutant_4.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
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GSM866748 | GPL1261 |
|
mutant tongue at E13.5, biological rep5
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Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/flox
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866748
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866748/suppl/GSM866748_Mutant_5.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
|
GSM866749 | GPL1261 |
|
mutant tongue at E13.5, biological rep6
|
Tongue from mouse embryos at day E13.5 of development
|
tissue: tongue
genotype: Myf5-Cre;Smad4flox/flox
age: E13.5
genetic background: C57BL/6J
|
Gene expression data from embryos in slow phase of cellularisation.
|
Sample_geo_accession | GSM866749
| Sample_status | Public on Feb 03 2012
| Sample_submission_date | Jan 26 2012
| Sample_last_update_date | Sep 12 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | RNA was isolated from tongue primordia at day 13.5 of development using RNeasy Mini Kits (Qiagen) and the manufacturer's recommended protocols.
| Sample_growth_protocol_ch1 | All animals were maintained at the University of Southern California.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kits (Qiagen) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, according to manufacturer's recommended protocols. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturer's recommended protocols.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
| Sample_data_processing | We used WebArray software (www.webarraydb.org/) to generate scaled log2 transformed gene expression values based on the RMA algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866749/suppl/GSM866749_Mutant_6.CEL.gz
| Sample_series_id | GSE35357
| Sample_data_row_count | 45101
| |
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