Search results for the GEO ID: GSE35414  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM867829 | GPL570 |
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MM1S_untreated_rep1
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Human Multiple Myeloma cell line MM1S
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time of treatment with fk866: 0 hours
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| Sample_geo_accession | GSM867829
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Jan 30 2012
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated cells were cultured in presence of FK866 at concentration of 10nM for 6 and 24 hours.
| | Sample_growth_protocol_ch1 | MM1s were cultured in RPMI-1640 medium supplemented with 10% of FBS, 100U/ml penicillin, 100µg/ml streptomycin and 2mMol/L L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAasi plus mini kit from Qiagen according to the manufactor's instruction
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
| | Sample_hyb_protocol | Fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a microarray chip overnight at 45°C. The hybridization solution is then removed.
| | Sample_scan_protocol | cRNA bound at its complementary oligonucleotide is excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions are captured.
| | Sample_data_processing | The data were analyzed with DNA-chip analyzer (dChip program) Arrays were normalized based on relative signal produced for an invariant subset of genes
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michele,,Cea
| | Sample_contact_email | michele_cea@dfci.harvard.edu
| | Sample_contact_laboratory | M551
| | Sample_contact_department | Medical oncology
| | Sample_contact_institute | Dana farber Cancer institute
| | Sample_contact_address | 450 Brookline Av
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM867nnn/GSM867829/suppl/GSM867829.CEL.gz
| | Sample_series_id | GSE35414
| | Sample_data_row_count | 54675
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GSM867830 | GPL570 |
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MM1S_fk866 6h_rep1
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Human Multiple Myeloma cell line MM1S
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time of treatment with fk866: 6 hours
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| Sample_geo_accession | GSM867830
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Jan 30 2012
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated cells were cultured in presence of FK866 at concentration of 10nM for 6 and 24 hours.
| | Sample_growth_protocol_ch1 | MM1s were cultured in RPMI-1640 medium supplemented with 10% of FBS, 100U/ml penicillin, 100µg/ml streptomycin and 2mMol/L L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAasi plus mini kit from Qiagen according to the manufactor's instruction
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
| | Sample_hyb_protocol | Fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a microarray chip overnight at 45°C. The hybridization solution is then removed.
| | Sample_scan_protocol | cRNA bound at its complementary oligonucleotide is excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions are captured.
| | Sample_data_processing | The data were analyzed with DNA-chip analyzer (dChip program) Arrays were normalized based on relative signal produced for an invariant subset of genes
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michele,,Cea
| | Sample_contact_email | michele_cea@dfci.harvard.edu
| | Sample_contact_laboratory | M551
| | Sample_contact_department | Medical oncology
| | Sample_contact_institute | Dana farber Cancer institute
| | Sample_contact_address | 450 Brookline Av
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM867nnn/GSM867830/suppl/GSM867830.CEL.gz
| | Sample_series_id | GSE35414
| | Sample_data_row_count | 54675
| | |
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GSM867831 | GPL570 |
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MM1S_fk866 24h_rep1
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Human Multiple Myeloma cell line MM1S
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time of treatment with fk866: 24 hours
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| Sample_geo_accession | GSM867831
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Jan 30 2012
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated cells were cultured in presence of FK866 at concentration of 10nM for 6 and 24 hours.
| | Sample_growth_protocol_ch1 | MM1s were cultured in RPMI-1640 medium supplemented with 10% of FBS, 100U/ml penicillin, 100µg/ml streptomycin and 2mMol/L L-glutamine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAasi plus mini kit from Qiagen according to the manufactor's instruction
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
| | Sample_hyb_protocol | Fragmented cRNA is added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a microarray chip overnight at 45°C. The hybridization solution is then removed.
| | Sample_scan_protocol | cRNA bound at its complementary oligonucleotide is excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions are captured.
| | Sample_data_processing | The data were analyzed with DNA-chip analyzer (dChip program) Arrays were normalized based on relative signal produced for an invariant subset of genes
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michele,,Cea
| | Sample_contact_email | michele_cea@dfci.harvard.edu
| | Sample_contact_laboratory | M551
| | Sample_contact_department | Medical oncology
| | Sample_contact_institute | Dana farber Cancer institute
| | Sample_contact_address | 450 Brookline Av
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM867nnn/GSM867831/suppl/GSM867831.CEL.gz
| | Sample_series_id | GSE35414
| | Sample_data_row_count | 54675
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