Search results for the GEO ID: GSE35415  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM867832 | GPL1261 |
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UTX conditional knockout (cKO) cell line 1
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embryonic stem cells
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cell: ES cells
genotype/variation: UTX conditional knockout
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Gene expression data from cKO1 ES cells
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| Sample_geo_accession | GSM867832
| | Sample_status | Public on Sep 04 2012
| | Sample_submission_date | Jan 30 2012
| | Sample_last_update_date | Sep 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse ES cells were maintained on irradiated embryonic fibroblast feeder cells and cultured in ES medium (DMEM supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 u/ml Penicillin/Streptomycin, 15% ES cell-qualified FBS, and 1000 u/ml LIF (Millipore)). Cells were passaged twice on 0.1% gelatin-coated dishes to remove feeder cells and then cultured in ES medium on gelatin-coated dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix label protocol
| | Sample_hyb_protocol | standard Affymetrix hyb protocol
| | Sample_scan_protocol | standard Affymetrix scan protocol
| | Sample_data_processing | The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chaochen,,Wang
| | Sample_contact_department | LERB
| | Sample_contact_institute | NIDDK
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM867nnn/GSM867832/suppl/GSM867832.CEL.gz
| | Sample_series_id | GSE35415
| | Sample_data_row_count | 45101
| | |
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GSM867833 | GPL1261 |
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UTX conditional knockout (cKO) cell line 2
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embryonic stem cells
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cell: ES cells
genotype/variation: UTX conditional knockout
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Gene expression data from cKO2 ES cells
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| Sample_geo_accession | GSM867833
| | Sample_status | Public on Sep 04 2012
| | Sample_submission_date | Jan 30 2012
| | Sample_last_update_date | Sep 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse ES cells were maintained on irradiated embryonic fibroblast feeder cells and cultured in ES medium (DMEM supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 u/ml Penicillin/Streptomycin, 15% ES cell-qualified FBS, and 1000 u/ml LIF (Millipore)). Cells were passaged twice on 0.1% gelatin-coated dishes to remove feeder cells and then cultured in ES medium on gelatin-coated dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix label protocol
| | Sample_hyb_protocol | standard Affymetrix hyb protocol
| | Sample_scan_protocol | standard Affymetrix scan protocol
| | Sample_data_processing | The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chaochen,,Wang
| | Sample_contact_department | LERB
| | Sample_contact_institute | NIDDK
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM867nnn/GSM867833/suppl/GSM867833.CEL.gz
| | Sample_series_id | GSE35415
| | Sample_data_row_count | 45101
| | |
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GSM867834 | GPL1261 |
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UTX knockout (KO) cell line 1
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embryonic stem cells
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cell: ES cells
genotype/variation: UTX knockout
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Gene expression data from KO1 ES cells
|
| Sample_geo_accession | GSM867834
| | Sample_status | Public on Sep 04 2012
| | Sample_submission_date | Jan 30 2012
| | Sample_last_update_date | Sep 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse ES cells were maintained on irradiated embryonic fibroblast feeder cells and cultured in ES medium (DMEM supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 u/ml Penicillin/Streptomycin, 15% ES cell-qualified FBS, and 1000 u/ml LIF (Millipore)). Cells were passaged twice on 0.1% gelatin-coated dishes to remove feeder cells and then cultured in ES medium on gelatin-coated dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix label protocol
| | Sample_hyb_protocol | standard Affymetrix hyb protocol
| | Sample_scan_protocol | standard Affymetrix scan protocol
| | Sample_data_processing | The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chaochen,,Wang
| | Sample_contact_department | LERB
| | Sample_contact_institute | NIDDK
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM867nnn/GSM867834/suppl/GSM867834.CEL.gz
| | Sample_series_id | GSE35415
| | Sample_data_row_count | 45101
| | |
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GSM867835 | GPL1261 |
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UTX knockout (KO) cell line 2
|
embryonic stem cells
|
cell: ES cells
genotype/variation: UTX knockout
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Gene expression data from KO2 ES cells
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| Sample_geo_accession | GSM867835
| | Sample_status | Public on Sep 04 2012
| | Sample_submission_date | Jan 30 2012
| | Sample_last_update_date | Sep 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse ES cells were maintained on irradiated embryonic fibroblast feeder cells and cultured in ES medium (DMEM supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 u/ml Penicillin/Streptomycin, 15% ES cell-qualified FBS, and 1000 u/ml LIF (Millipore)). Cells were passaged twice on 0.1% gelatin-coated dishes to remove feeder cells and then cultured in ES medium on gelatin-coated dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard Affymetrix label protocol
| | Sample_hyb_protocol | standard Affymetrix hyb protocol
| | Sample_scan_protocol | standard Affymetrix scan protocol
| | Sample_data_processing | The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chaochen,,Wang
| | Sample_contact_department | LERB
| | Sample_contact_institute | NIDDK
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM867nnn/GSM867835/suppl/GSM867835.CEL.gz
| | Sample_series_id | GSE35415
| | Sample_data_row_count | 45101
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