Search results for the GEO ID: GSE35696  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM873553 | GPL570 |
|
RL_siRNA_NS_rep1
|
MCF7 cells, RL silenced, NS
|
cell line: MCF7 breast cancer
treatment: RL silenced
|
|
| Sample_geo_accession | GSM873553
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM Renilla Luciferase siRNA. 72h later, cells were cutured 4h without FCS
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873553/suppl/GSM873553.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873554 | GPL570 |
|
RL_siRNA_LIF1h_rep1
|
MCF7 cells, RL silenced, LIF 1h
|
cell line: MCF7 breast cancer
treatment: RL silenced and LIF-stimulated
|
|
| Sample_geo_accession | GSM873554
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM Renilla Luciferase siRNA. 72h later, cells were cutured 4h without FCS and stimulated with 10ng/ml LIF 1h
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873554/suppl/GSM873554.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873555 | GPL570 |
|
SIN3A_siRNA_NS_rep1
|
MCF7 cells, SIN3A silenced, NS
|
cell line: MCF7 breast cancer
treatment: SIN3A silenced
|
|
| Sample_geo_accession | GSM873555
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM SIN3A siRNA. 72h later, cells were cutured 4h without FCS
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873555/suppl/GSM873555.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873556 | GPL570 |
|
RL_siRNA_NS_rep2
|
MCF7 cells, RL silenced, NS
|
cell line: MCF7 breast cancer
treatment: RL silenced
|
|
| Sample_geo_accession | GSM873556
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM Renilla Luciferase siRNA. 72h later, cells were cutured 4h without FCS
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873556/suppl/GSM873556.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873557 | GPL570 |
|
RL_siRNA_LIF1h_rep2
|
MCF7 cells, RL silenced, LIF 1h
|
cell line: MCF7 breast cancer
treatment: RL silenced and LIF-stimulated
|
|
| Sample_geo_accession | GSM873557
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM Renilla Luciferase siRNA. 72h later, cells were cutured 4h without FCS and stimulated with 10ng/ml LIF 1h
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873557/suppl/GSM873557.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873558 | GPL570 |
|
SIN3A_siRNA_NS_rep2
|
MCF7 cells, SIN3A silenced, NS
|
cell line: MCF7 breast cancer
treatment: SIN3A silenced
|
|
| Sample_geo_accession | GSM873558
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM SIN3A siRNA. 72h later, cells were cutured 4h without FCS
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873558/suppl/GSM873558.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873559 | GPL570 |
|
SIN3A_siRNA_LIF1h_rep1
|
MCF7 cells, SIN3A silenced, LIF 1h
|
cell line: MCF7 breast cancer
treatment: SIN3A silenced and LIF-stimulated
|
|
| Sample_geo_accession | GSM873559
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM SIN3A siRNA. 72h later, cells were cutured 4h without FCS and stimulated with 10ng/ml LIF 1h
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873559/suppl/GSM873559.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873560 | GPL570 |
|
RL_siRNA_NS_rep3
|
MCF7 cells, RL silenced, NS
|
cell line: MCF7 breast cancer
treatment: RL silenced
|
|
| Sample_geo_accession | GSM873560
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM Renilla Luciferase siRNA. 72h later, cells were cutured 4h without FCS
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873560/suppl/GSM873560.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873561 | GPL570 |
|
RL_siRNA_LIF1h_rep3
|
MCF7 cells, RL silenced, LIF 1h
|
cell line: MCF7 breast cancer
treatment: RL silenced and LIF-stimulated
|
|
| Sample_geo_accession | GSM873561
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM Renilla Luciferase siRNA. 72h later, cells were cutured 4h without FCS and stimulated with 10ng/ml LIF 1h
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873561/suppl/GSM873561.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873562 | GPL570 |
|
SIN3A_siRNA_NS_rep3
|
MCF7 cells, SIN3A silenced, NS
|
cell line: MCF7 breast cancer
treatment: SIN3A silenced
|
|
| Sample_geo_accession | GSM873562
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM SIN3A siRNA. 72h later, cells were cutured 4h without FCS
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873562/suppl/GSM873562.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
| | |
|
GSM873563 | GPL570 |
|
SIN3A_siRNA_LIF1h_rep2
|
MCF7 cells, SIN3A silenced, LIF 1h
|
cell line: MCF7 breast cancer
treatment: SIN3A silenced and LIF-stimulated
|
|
| Sample_geo_accession | GSM873563
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
| | Sample_treatment_protocol_ch1 | Transfected with 50nM SIN3A siRNA. 72h later, cells were cutured 4h without FCS and stimulated with 10ng/ml LIF 1h
| | Sample_growth_protocol_ch1 | DMEM+10%FCS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joke,,Allemeersch
| | Sample_contact_email | joke.allemeersch@vib.be
| | Sample_contact_phone | +32(0)16 37 31 26
| | Sample_contact_department | Nucleomics Core
| | Sample_contact_institute | Flanders Institute for Biotechnology (VIB)
| | Sample_contact_address | Herestraat 49 Box 816
| | Sample_contact_city | Leuven
| | Sample_contact_zip/postal_code | B-3000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873563/suppl/GSM873563.CEL.gz
| | Sample_series_id | GSE35696
| | Sample_data_row_count | 54675
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