Search results for the GEO ID: GSE35712  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM873800 | GPL570 |
|
UPN727 cells, autologous plasma, flu, biological replicate 1
|
UPN727 cells stimulated with autologous plasma
|
responder cell line: UPN727
protocol: stimulated with autologous plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873800
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873800/suppl/GSM873800_1_autol_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873801 | GPL570 |
|
UPN727 cells, autologous plasma, flu, biological replicate 2
|
UPN727 cells stimulated with autologous plasma
|
responder cell line: UPN727
protocol: stimulated with autologous plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873801
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873801/suppl/GSM873801_2_autol_old_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873802 | GPL570 |
|
UPN727 cells, 1090A pre flu plasma
|
UPN727 cells stimulated with 1090A pre flu plasma
|
responder cell line: UPN727
protocol: stimulated with 1090A pre flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873802
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873802/suppl/GSM873802_3_1090A_pre_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873803 | GPL570 |
|
UPN727 cells, 1090B active flu plasma
|
UPN727 cells stimulated with 1090B active flu plasma
|
responder cell line: UPN727
protocol: stimulated with 1090B active flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873803
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873803/suppl/GSM873803_4_1090B_t0_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873804 | GPL570 |
|
UPN727 cells, 033S pre flu plasma
|
UPN727 cells stimulated with 033S pre flu plasma
|
responder cell line: UPN727
protocol: stimulated with 033S pre flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873804
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873804/suppl/GSM873804_10_033S_pre_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873805 | GPL570 |
|
UPN727 cells, 033T active flu plasma
|
UPN727 cells stimulated with 033T active flu plasma
|
responder cell line: UPN727
protocol: stimulated with 033T active flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873805
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873805/suppl/GSM873805_9_033T_t0_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873806 | GPL570 |
|
UPN727 cells, 121F pre flu plasma
|
UPN727 cells stimulated with 121F pre flu plasma
|
responder cell line: UPN727
protocol: stimulated with 121F pre flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873806
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873806/suppl/GSM873806_13_121F_pre_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873807 | GPL570 |
|
UPN727 cells, 121G active flu plasma
|
UPN727 cells stimulated with 121G active flu plasma
|
responder cell line: UPN727
protocol: stimulated with 121G active flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873807
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873807/suppl/GSM873807_12_121G_t0_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873808 | GPL570 |
|
UPN727 cells, 1223A pre flu plasma
|
UPN727 cells stimulated with 1223A pre flu plasma
|
responder cell line: UPN727
protocol: stimulated with 1223A pre flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873808
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873808/suppl/GSM873808_14_1223A_pre_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873809 | GPL570 |
|
UPN727 cells, 1223B active flu plasma
|
UPN727 cells stimulated with 1223B active flu plasma
|
responder cell line: UPN727
protocol: stimulated with 1223B active flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873809
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873809/suppl/GSM873809_15_1223B_t0_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873810 | GPL570 |
|
UPN727 cells, 1052A pre flu plasma
|
UPN727 cells stimulated with 1052A pre flu plasma
|
responder cell line: UPN727
protocol: stimulated with 1052A pre flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873810
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873810/suppl/GSM873810_18_1052A_pre_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
GSM873811 | GPL570 |
|
UPN727 cells, 1052B active flu plasma
|
UPN727 cells stimulated with 1052B active flu plasma
|
responder cell line: UPN727
protocol: stimulated with 1052B active flu plasma
|
Gene expression data from UPN727 cells stimulated with pre vs. active H1N1 (flu) plasma
|
| Sample_geo_accession | GSM873811
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Feb 10 2012
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Gene expression was accomplished by culturing PBMCs at 37°C in 5% CO2 with 40% of autologous or H1N1 sera. Cultures were prepared in a Costar 24-well plate (Corning) using 500,000 cells/well and in RPMI 1640 medium supplemented with 100U/ml penicillin and 100ug/ml streptomycin in a total of 500 µl. After culture (9 hours optimal for cryopreserved PBMCs) total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies).
| | Sample_growth_protocol_ch1 | Commercial cryopreserved PBMCs of healthy Caucasian male donor UPN727 were thawed and washed per the manufacturer’s protocol (Cellular Technology Ltd., Shaker Heights, OH).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
| | Sample_hyb_protocol | The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
| | Sample_scan_protocol | After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
| | Sample_data_processing | Image data were normalized and analyzed Partek Genomic Suite (Partek GS) to determine signal log ratios. Each sample was independently analyzed. The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Paired 2 sample ttest as a statistic analysis method in Partek GS.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Martin,,Hessner
| | Sample_contact_email | mhessner@mcw.edu
| | Sample_contact_phone | 414-456-4496
| | Sample_contact_fax | 414-456-6663
| | Sample_contact_laboratory | Max McGee National Research Center for Juvenile Diabetes
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | Medical College of Wisconsin
| | Sample_contact_address | 8701 Watertown Plank Road
| | Sample_contact_city | Milwaukee
| | Sample_contact_state | WI
| | Sample_contact_zip/postal_code | 53226
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mcw.edu/MaxMcGeeResearchCenter.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM873nnn/GSM873811/suppl/GSM873811_19_1052B_t0_727_9hr_flu_12-9-09.CEL.gz
| | Sample_series_id | GSE35712
| | Sample_series_id | GSE35713
| | Sample_data_row_count | 54675
| | |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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