Result

Search results for the GEO ID: GSE35755

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GSM ID

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Title

Source name

Description

Characteristics

GSM874443

GPL1261

Control (hetzerozygous littermates)

primary sertoli cells from 3 days old mice

strain: C57BL/6 genotype/variation: control age: 3 days old cell type: primary sertoli cells

Gene expression data from primary sertoli cells of 3 days old mice

Sample_geo_accession	GSM874443
Sample_status	Public on Feb 13 2012
Sample_submission_date	Feb 13 2012
Sample_last_update_date	Feb 14 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	3 days old mice were anesthetized with CO2 and then killed by cervical dislocation. Decapsulated testes were incubated with 2 mg/ml collagena (Gibco, Invitrogen, Carlsbad, CA, USA) at room temperature for 10 min with gentle oscillation and then filtered through 80 um copper meshes to remove interstitial cells. The seminiferous tubules were resuspended in the collagenase at room temperature for an additional 15 min to remove myoid cells. The tubules were then incubated with 0.5 mg/ml hyaluronidase (Sigma, St. Louis, MO, USA) at room temperature for 15 min with gentle oscillation and pipetting. After washing twice with PBS, the cells were cultured in DMEM /F12 (Sigma, St. Louis, MO, USA) supplemented with sodium bicarbonate (1.2 mg/ml), penicillin (100 U/ml), streptomycin (100 mg/ml), and 10% fetal bovine serum (Life Technologies, Gaithersburg, MD, USA). The cells were maintained in a humidified atmosphere containing5% CO2 at 37 ℃ for 24 h. After the incubation, cells were treated with a hypotonic solution (20 mM Tris, pH 7.4) for 1 min to remove the spermatogenic cells adhering to the Sertoli cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	After cultured sertoli cell twenty-four hours, cell were washed with PBS and placed on ice in the Trizol solution (invitrogen,Carlsbad, CA, USA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (3_ivt_express_kit_manual Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 7G.
Sample_data_processing	The data were analyzed with PARTEK genomic suit 6.4 using RMA normalization
Sample_platform_id	GPL1261
Sample_contact_name	Xiu-Xing,,Wang
Sample_contact_email	xiuxing81@yahoo.com.cn
Sample_contact_phone	862583596289
Sample_contact_fax	862583596289
Sample_contact_department	MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center and the School of Medicine
Sample_contact_institute	Nanjing University
Sample_contact_address	#22 Hankou Road
Sample_contact_city	Nanjing
Sample_contact_state	Jiangsu
Sample_contact_zip/postal_code	210093
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874443/suppl/GSM874443.CEL.gz
Sample_series_id	GSE35755
Sample_data_row_count	45101

GSM874444

GPL1261

primary sertoli cells from 3 days old mice

strain: C57BL/6 genotype/variation: GGPP knockout age: 3 days old cell type: primary sertoli cells

Gene expression data from primary sertoli cells of 3 days old mice

Sample_geo_accession	GSM874444
Sample_status	Public on Feb 13 2012
Sample_submission_date	Feb 13 2012
Sample_last_update_date	Feb 14 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	3 days old mice were anesthetized with CO2 and then killed by cervical dislocation. Decapsulated testes were incubated with 2 mg/ml collagena (Gibco, Invitrogen, Carlsbad, CA, USA) at room temperature for 10 min with gentle oscillation and then filtered through 80 um copper meshes to remove interstitial cells. The seminiferous tubules were resuspended in the collagenase at room temperature for an additional 15 min to remove myoid cells. The tubules were then incubated with 0.5 mg/ml hyaluronidase (Sigma, St. Louis, MO, USA) at room temperature for 15 min with gentle oscillation and pipetting. After washing twice with PBS, the cells were cultured in DMEM /F12 (Sigma, St. Louis, MO, USA) supplemented with sodium bicarbonate (1.2 mg/ml), penicillin (100 U/ml), streptomycin (100 mg/ml), and 10% fetal bovine serum (Life Technologies, Gaithersburg, MD, USA). The cells were maintained in a humidified atmosphere containing5% CO2 at 37 ℃ for 24 h. After the incubation, cells were treated with a hypotonic solution (20 mM Tris, pH 7.4) for 1 min to remove the spermatogenic cells adhering to the Sertoli cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	After cultured sertoli cell twenty-four hours, cell were washed with PBS and placed on ice in the Trizol solution (invitrogen,Carlsbad, CA, USA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (3_ivt_express_kit_manual Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 7G.
Sample_data_processing	The data were analyzed with PARTEK genomic suit 6.4 using RMA normalization
Sample_platform_id	GPL1261
Sample_contact_name	Xiu-Xing,,Wang
Sample_contact_email	xiuxing81@yahoo.com.cn
Sample_contact_phone	862583596289
Sample_contact_fax	862583596289
Sample_contact_department	MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center and the School of Medicine
Sample_contact_institute	Nanjing University
Sample_contact_address	#22 Hankou Road
Sample_contact_city	Nanjing
Sample_contact_state	Jiangsu
Sample_contact_zip/postal_code	210093
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874444/suppl/GSM874444.CEL.gz
Sample_series_id	GSE35755
Sample_data_row_count	45101

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