Search results for the GEO ID: GSE35785  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM874936 | GPL1261 |
|
AGH-OG-1
|
haploid embryonic stem cells derived from androgenetic blastocyst
|
karotype: haploid
cell type: embryonic stem cells
genotype: C57BL/6J
genotype: Oct4-EGFP transgene
passage: P14
|
Gene expression data from androgenetic haploid embryonic stem cells
|
| Sample_geo_accession | GSM874936
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874936/suppl/GSM874936.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874936/suppl/GSM874936.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
GSM874937 | GPL1261 |
|
AGH-OG-2
|
haploid embryonic stem cells derived from androgenetic blastocyst
|
karotype: haploid
cell type: embryonic stem cells
genotype: C57BL/6J
genotype: Oct4-EGFP transgene
passage: P15
|
Gene expression data from androgenetic haploid embryonic stem cells
|
| Sample_geo_accession | GSM874937
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874937/suppl/GSM874937.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874937/suppl/GSM874937.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
GSM874938 | GPL1261 |
|
AGH-EG-1
|
haploid embryonic stem cells derived from androgenetic blastocyst
|
karotype: haploid
cell type: embryonic stem cells
genotype: C57BL/6J
genotype: Actin-EGFP transgene
passage: P15
|
Gene expression data from androgenetic haploid embryonic stem cells
|
| Sample_geo_accession | GSM874938
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874938/suppl/GSM874938.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874938/suppl/GSM874938.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
GSM874939 | GPL1261 |
|
AGH-OG-3
|
haploid embryonic stem cells derived from androgenetic blastocyst
|
karotype: haploid
cell type: embryonic stem cells
genotype: C57BL/6J
genotype: Oct4-EGFP transgene
passage: P12
|
Gene expression data from androgenetic haploid embryonic stem cells
|
| Sample_geo_accession | GSM874939
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874939/suppl/GSM874939.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874939/suppl/GSM874939.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
GSM874940 | GPL1261 |
|
AGH-OG-4
|
haploid embryonic stem cells derived from androgenetic blastocyst
|
karotype: haploid
cell type: embryonic stem cells
genotype: C57BL/6J
genotype: Oct4-EGFP transgene
passage: P14
|
Gene expression data from androgenetic haploid embryonic stem cells
|
| Sample_geo_accession | GSM874940
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874940/suppl/GSM874940.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874940/suppl/GSM874940.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
GSM874941 | GPL1261 |
|
E14, biological rep1
|
diploid male embryonic stem cells
|
karotype: diploid
cell type: embryonic stem cells
genotype: 129/Sv
genotype: wild type
passage: P17
|
Gene expression data from E14
|
| Sample_geo_accession | GSM874941
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874941/suppl/GSM874941.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874941/suppl/GSM874941.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
GSM874942 | GPL1261 |
|
E14, biological rep2
|
diploid male embryonic stem cells
|
karotype: diploid
cell type: embryonic stem cells
genotype: 129/Sv
genotype: wild type
passage: P17
|
Gene expression data from E14
|
| Sample_geo_accession | GSM874942
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874942/suppl/GSM874942.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874942/suppl/GSM874942.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
GSM874943 | GPL1261 |
|
E14, biological rep3
|
diploid male embryonic stem cells
|
karotype: diploid
cell type: embryonic stem cells
genotype: 129/Sv
genotype: wild type
passage: P17
|
Gene expression data from E14
|
| Sample_geo_accession | GSM874943
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874943/suppl/GSM874943.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874943/suppl/GSM874943.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
GSM874944 | GPL1261 |
|
MEF,biological rep1
|
MEFs isolated from a male d13.5 embryo
|
karotype: diploid
cell type: somatic cells
genotype: B6D2F1
genotype: wild type
passage: P2
|
Gene expression data from MEF derived from male d13.5 embyos
|
| Sample_geo_accession | GSM874944
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874944/suppl/GSM874944.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874944/suppl/GSM874944.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
GSM874945 | GPL1261 |
|
MEF,biological rep2
|
MEFs isolated from a male d13.5 embryo
|
karotype: diploid
cell type: somatic cells
genotype: B6D2F1
genotype: wild type
passage: P2
|
Gene expression data from MEF derived from male d13.5 embyos
|
| Sample_geo_accession | GSM874945
| | Sample_status | Public on Apr 27 2012
| | Sample_submission_date | Feb 13 2012
| | Sample_last_update_date | Apr 27 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To sort haploid cells, ES cells were trypsinized, washed by DPBS (GIBCO), and then incubated with 15 μg/ml Hoechst 33342 in 37°C water bath. Subsequently, the haploid 1n peak was purified using BD FACS AriaII for microarry analysis. For E14 and MEF, the diploid 2n peak was purified. All the cells were preserved in -80°C.
| | Sample_growth_protocol_ch1 | Androgeneic haploid embryonic stem cells (AG-haESCs) were cultured on feeder in ES medium supplemented with CHIR99021 and PD0325901.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using RNeasy mini kit(Cat#74106, QIAGEN, GmBH,Germany) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Linyu,,Shi
| | Sample_contact_laboratory | Mechanism and Application of Somatic Reprogramming
| | Sample_contact_institute | Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
| | Sample_contact_address | 320 Yueyang Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200031
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874945/suppl/GSM874945.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM874nnn/GSM874945/suppl/GSM874945.CHP.gz
| | Sample_series_id | GSE35785
| | Sample_series_id | GSE35787
| | Sample_data_row_count | 45101
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
