Search results for the GEO ID: GSE35932  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM877586 | GPL570 |
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Human endothelial cell Hypoxia 0hr
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HUVEC, Hypoxia 0hr
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cell line: HUVEC
treatment: Hypoxia
time period: 0hr
|
|
| Sample_geo_accession | GSM877586
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Feb 20 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM877nnn/GSM877586/suppl/GSM877586.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921645 | GPL570 |
|
Hypoxia profile
|
HUVEC, hypoxia 1hr
|
cell line: HUVEC
treatment: hypoxia
time period: 1hr
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|
| Sample_geo_accession | GSM921645
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921645/suppl/GSM921645.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921646 | GPL570 |
|
Hypoxia profile 2hrs
|
HUVEC, hypoxia 2hrs
|
cell line: HUVEC
treatment: hypoxia
time period: 2hrs
|
|
| Sample_geo_accession | GSM921646
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921646/suppl/GSM921646.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921647 | GPL570 |
|
Hypoxia profiles 4hrs
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HUVEC, hypoxia 4hrs
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cell line: HUVEC
treatment: hypoxia
time period: 4hrs
|
|
| Sample_geo_accession | GSM921647
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921647/suppl/GSM921647.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921649 | GPL570 |
|
Hypoxia profile 8hrs
|
HUVEC, hypoxia 8hrs
|
cell line: HUVEC
treatment: hypoxia
time period: 8hrs
|
|
| Sample_geo_accession | GSM921649
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921649/suppl/GSM921649.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921651 | GPL570 |
|
Hypoxia profile 12hrs
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HUVEC, hypoxia 12hrs
|
cell line: HUVEC
treatment: hypoxia
time period: 12hrs
|
|
| Sample_geo_accession | GSM921651
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921651/suppl/GSM921651.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921652 | GPL570 |
|
HUVEC profile 24hrs
|
HUVEC, hypoxia 24hrs
|
cell line: HUVEC
treatment: hypoxia
time period: 24hrs
|
|
| Sample_geo_accession | GSM921652
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921652/suppl/GSM921652.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921661 | GPL570 |
|
HIF1array_CtlsiRNA_Normoxia
|
HUVEC, HIF1array_CtlsiRNA_Normoxia
|
cell line: HUVEC
sirna: CtlsiRNA
treatment: absensce of hypoxia
|
|
| Sample_geo_accession | GSM921661
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921661/suppl/GSM921661.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921662 | GPL570 |
|
HIF1array_HIF1siRNA_Normoxia
|
HUVEC, HIF1array_HIF1siRNA_Normoxia
|
cell line: HUVEC
sirna: HIF1siRNA
treatment: absensce of hypoxia
|
|
| Sample_geo_accession | GSM921662
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921662/suppl/GSM921662.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921663 | GPL570 |
|
HUVEC HIF1array_CtlsiRNA_Hypoxia
|
HUVEC HIF1array_CtlsiRNA_Hypoxia
|
cell line: HUVEC
sirna: CtlsiRNA
treatment: presence of hypoxia
|
|
| Sample_geo_accession | GSM921663
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921663/suppl/GSM921663.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921664 | GPL570 |
|
HUVEC HIF1array_HIF1siRNA_Hypoxia
|
HUVEC HIF1array_HIF1siRNA_Hypoxia
|
cell line: HUVEC
sirna: HIF1siRNA
treatment: presence of hypoxia
|
|
| Sample_geo_accession | GSM921664
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921664/suppl/GSM921664.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921665 | GPL570 |
|
HUVEC KDM3Aarray_CtlsiRNA_Normoxia
|
HUVEC KDM3Aarray_CtlsiRNA_Normoxia
|
cell line: HUVEC
sirna: CtlsiRNA
treatment: absensce of hypoxia
|
|
| Sample_geo_accession | GSM921665
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921665/suppl/GSM921665.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921666 | GPL570 |
|
HUVEC KDM3Aarray_KDM3AsiRNA_Normoxia
|
HUVEC KDM3Aarray_KDM3AsiRNA_Normoxia
|
cell line: HUVEC
sirna: KDM3AsiRNA
treatment: absensce of hypoxia
|
|
| Sample_geo_accession | GSM921666
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921666/suppl/GSM921666.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921667 | GPL570 |
|
HUVEC KDM3Aarray_CtlsiRNA_Hypoxia
|
HUVEC KDM3Aarray_CtlsiRNA_Hypoxia
|
cell line: HUVEC
sirna: CtlsiRNA
treatment: presence of hypoxia
|
|
| Sample_geo_accession | GSM921667
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921667/suppl/GSM921667.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
|
GSM921668 | GPL570 |
|
HUVEC KDM3Aarray_KDM3AsiRNA_Hypoxia
|
HUVEC KDM3Aarray_KDM3AsiRNA_Hypoxia
|
cell line: HUVEC
sirna: KDM3AsiRNA
treatment: presence of hypoxia
|
|
| Sample_geo_accession | GSM921668
| | Sample_status | Public on Jun 07 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Jun 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Imari,,Mimura
| | Sample_contact_department | Laboratory for Systems Biology and Medicine
| | Sample_contact_institute | Research Center for Advanced Science and Technology
| | Sample_contact_address | 4-6-1 Komaba Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921668/suppl/GSM921668.CEL.gz
| | Sample_series_id | GSE35932
| | Sample_data_row_count | 54675
| | |
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