Search results for the GEO ID: GSE35959  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM878095 | GPL570 |
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hMSC-C_donor1(#276)_P1_42yrs
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hMSC-C_middle-aged
|
gender: female
age: 42 yrs (middle-aged)
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 1 (MSC population #276)
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hMSC-C_1
|
| Sample_geo_accession | GSM878095
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence, human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit and the GeneChip 3’IVT Express Kit starting from 2 µg total RN , respectively (both from Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878095/suppl/GSM878095_hMSC-C_1.CEL.gz
| | Sample_series_id | GSE35955
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878096 | GPL570 |
|
hMSC-C_donor2(#353)_P2_67yrs
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hMSC-C_middle-aged
|
gender: female
age: 67 yrs (middle-aged)
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 2
sample id: donor 2 (MSC population #353)
|
hMSC-C_2
|
| Sample_geo_accession | GSM878096
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence, human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit and the GeneChip 3’IVT Express Kit starting from 2 µg total RN , respectively (both from Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878096/suppl/GSM878096_hMSC-C_2.CEL.gz
| | Sample_series_id | GSE35955
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878097 | GPL570 |
|
hMSC-C_donor3(#295)_P1_61yrs
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hMSC-C_middle-aged
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gender: male
age: 61 yrs (middle-aged)
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 3 (MSC population #295)
|
hMSC-C_3
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| Sample_geo_accession | GSM878097
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence, human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit and the GeneChip 3’IVT Express Kit starting from 2 µg total RN , respectively (both from Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878097/suppl/GSM878097_hMSC-C_3.CEL.gz
| | Sample_series_id | GSE35955
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878098 | GPL570 |
|
hMSC-C_donor4(#296)_P1_62yrs
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hMSC-C_middle-aged
|
gender: female
age: 62 yrs (middle-aged)
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 4 (MSC population #296)
|
hMSC-C_4
|
| Sample_geo_accession | GSM878098
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence, human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit and the GeneChip 3’IVT Express Kit starting from 2 µg total RN , respectively (both from Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878098/suppl/GSM878098_hMSC-C_4.CEL.gz
| | Sample_series_id | GSE35955
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878099 | GPL570 |
|
hMSC-C_donor5(#247)_P1_56yrs
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hMSC-C_middle-aged
|
gender: female
age: 56 yrs (middle-aged)
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 5 (MSC population #247)
|
hMSC-C_5
|
| Sample_geo_accession | GSM878099
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence, human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit and the GeneChip 3’IVT Express Kit starting from 2 µg total RN , respectively (both from Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878099/suppl/GSM878099_hMSC-C_5.CEL.gz
| | Sample_series_id | GSE35955
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878100 | GPL570 |
|
hMSC-old_donor1(#520)_P1_79yrs
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hMSC-old_elderly
|
gender: female
age: 79 yrs (elderly-aged)
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 1 (MSC population #520)
|
hMSC-old_1
|
| Sample_geo_accession | GSM878100
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence, human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the GeneChip 3' IVT Express Kit (Affymetrix, High Wycombe, United Kingdom) starting from 200ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878100/suppl/GSM878100_hMSC-old_1.CEL.gz
| | Sample_series_id | GSE35955
| | Sample_series_id | GSE35958
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878101 | GPL570 |
|
hMSC-old_donor2(#559)_P1_79yrs
|
hMSC-old_elderly
|
gender: male
age: 79 yrs (elderly-aged)
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 2 (MSC population #559)
|
hMSC-old_2
|
| Sample_geo_accession | GSM878101
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence, human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the GeneChip 3' IVT Express Kit (Affymetrix, High Wycombe, United Kingdom) starting from 200ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878101/suppl/GSM878101_hMSC-old_2.CEL.gz
| | Sample_series_id | GSE35955
| | Sample_series_id | GSE35958
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878102 | GPL570 |
|
hMSC-old_donor3(#606)_P1_80yrs
|
hMSC-old_elderly
|
gender: female
age: 80 yrs (elderly-aged)
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 3 (MSC population #606)
|
hMSC-old_3
|
| Sample_geo_accession | GSM878102
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence, human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the GeneChip 3' IVT Express Kit (Affymetrix, High Wycombe, United Kingdom) starting from 200ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878102/suppl/GSM878102_hMSC-old_3.CEL.gz
| | Sample_series_id | GSE35955
| | Sample_series_id | GSE35958
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878103 | GPL570 |
|
hMSC-old_donor4(#663)_P1_89yrs
|
hMSC-old_elderly
|
gender: female
age: 89 yrs (elderly-aged)
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 4 (MSC population #663)
|
hMSC-old_4
|
| Sample_geo_accession | GSM878103
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence, human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the GeneChip 3' IVT Express Kit (Affymetrix, High Wycombe, United Kingdom) starting from 200ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878103/suppl/GSM878103_hMSC-old_4.CEL.gz
| | Sample_series_id | GSE35955
| | Sample_series_id | GSE35958
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878104 | GPL570 |
|
hMSC-osteopo_donor1(#535)_P1_79yrs
|
hMSC-osteopo_elderly
|
gender: female
age: 79 yrs (elderly)
diagnosis: primary osteoporosis
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 1 with primary osteoporosis (MSC population #535)
|
hMSC-OP_1
|
| Sample_geo_accession | GSM878104
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed in P1 and P2, respectively. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878104/suppl/GSM878104_hMSC-OP_1.CEL.gz
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35958
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878105 | GPL570 |
|
hMSC-osteopo_donor2(#547)_P1_94yrs
|
hMSC-osteopo_elderly
|
gender: female
age: 94 yrs (elderly)
diagnosis: primary osteoporosis
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 2 with primary osteoporosis (MSC population #547)
|
hMSC-OP_2
|
| Sample_geo_accession | GSM878105
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed in P1 and P2, respectively. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878105/suppl/GSM878105_hMSC-OP_2.CEL.gz
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35958
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878106 | GPL570 |
|
hMSC-osteopo_donor3(#558)_P2_87yrs
|
hMSC-osteopo_elderly
|
gender: female
age: 87 yrs (elderly)
diagnosis: primary osteoporosis
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 2
sample id: donor 3 with primary osteoporosis (MSC population #558)
|
hMSC-OP_3
|
| Sample_geo_accession | GSM878106
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed in P1 and P2, respectively. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878106/suppl/GSM878106_hMSC-OP_3.CEL.gz
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35958
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878107 | GPL570 |
|
hMSC-osteopo_donor4(#572)_P1_82yrs
|
hMSC-osteopo_elderly
|
gender: female
age: 82 yrs (elderly)
diagnosis: primary osteoporosis
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 4 with primary osteoporosis (MSC population #572)
|
hMSC-OP_4
|
| Sample_geo_accession | GSM878107
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed in P1 and P2, respectively. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878107/suppl/GSM878107_hMSC-OP_4.CEL.gz
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35958
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878108 | GPL570 |
|
hMSC-osteopo_donor5(#573)_P1_89yrs
|
hMSC-osteopo_elderly
|
gender: female
age: 89 yrs (elderly)
diagnosis: primary osteoporosis
tissue: bone marrow
cell type: mesenchymal stem cells
passage: passage 1
sample id: donor 5 with primary osteoporosis (MSC population #573)
|
hMSC-OP_5
|
| Sample_geo_accession | GSM878108
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3 at least once.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed in P1 and P2, respectively. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878108/suppl/GSM878108_hMSC-OP_5.CEL.gz
| | Sample_series_id | GSE35956
| | Sample_series_id | GSE35958
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878109 | GPL570 |
|
hMSC-senescent_donor1(#276)_Px_42yrs
|
hMSC-senescent
|
gender: female
age: 42 yrs
tissue: bone marrow
cell type: mesenchymal stem cells
passage: long term-cultivation (cellular senescence state)
donor/sample id: donor 1 (MSC population #276)
|
hMSC-senescent_1
|
| Sample_geo_accession | GSM878109
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3. For hMSC-senescent, this procedure was repeated for up to x passages when the hMSC did not become confluent within 3 weeks due to replicative senescence. Control cells were reseeded until passage 1 and 2, respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878109/suppl/GSM878109_hMSC-senescent_donor1.CEL.gz
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878110 | GPL570 |
|
hMSC-senescent_donor2(#278)_Px_59yrs
|
hMSC-senescent
|
gender: female
age: 59 yrs
tissue: bone marrow
cell type: mesenchymal stem cells
passage: long term-cultivation (cellular senescence state)
donor/sample id: donor 2 (MSC population #278)
|
hMSC-senescent_2
|
| Sample_geo_accession | GSM878110
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3. For hMSC-senescent, this procedure was repeated for up to x passages when the hMSC did not become confluent within 3 weeks due to replicative senescence. Control cells were reseeded until passage 1 and 2, respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878110/suppl/GSM878110_hMSC-senescent_donor2.CEL.gz
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878111 | GPL570 |
|
hMSC-senescent_donor3(#271)_Px_64yrs
|
hMSC-senescent
|
gender: female
age: 64 yrs
tissue: bone marrow
cell type: mesenchymal stem cells
passage: long term-cultivation (cellular senescence state)
donor/sample id: donor 3 (MSC population #271)
|
hMSC-senescent_3
|
| Sample_geo_accession | GSM878111
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3. For hMSC-senescent, this procedure was repeated for up to x passages when the hMSC did not become confluent within 3 weeks due to replicative senescence. Control cells were reseeded until passage 1 and 2, respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878111/suppl/GSM878111_hMSC-senescent_donor3.CEL.gz
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
| | |
|
GSM878112 | GPL570 |
|
hMSC-senescent_donor4(#277)_Px_54yrs
|
hMSC-senescent
|
gender: male
age: 54 yrs
tissue: bone marrow
cell type: mesenchymal stem cells
passage: long term-cultivation (cellular senescence state)
donor/sample id: donor 4 (MSC population #277)
|
hMSC-senescent_4
|
| Sample_geo_accession | GSM878112
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3. For hMSC-senescent, this procedure was repeated for up to x passages when the hMSC did not become confluent within 3 weeks due to replicative senescence. Control cells were reseeded until passage 1 and 2, respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878112/suppl/GSM878112_hMSC-senescent_donor4.CEL.gz
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
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GSM878113 | GPL570 |
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hMSC-senescent_donor5(#274)_Px_63yrs
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hMSC-senescent
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gender: male
age: 63 yrs
tissue: bone marrow
cell type: mesenchymal stem cells
passage: long term-cultivation (cellular senescence state)
donor/sample id: donor 5 (MSC population #274)
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hMSC-senescent_5
|
| Sample_geo_accession | GSM878113
| | Sample_status | Public on Feb 22 2012
| | Sample_submission_date | Feb 21 2012
| | Sample_last_update_date | Feb 22 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cell culture medium, fetal calf serum (FCS), trypsin-EDTA and antibiotics were obtained from PAA Laboratories GmbH, Linz, Austria. Human MSC of bone marrow were selected by surface adherence and expanded in DMEM/Ham’s F-12 (1:1) medium supplemented with 10% heat-inactivated FCS, 1 U/ml penicillin, 100 µg/ml streptomycin and 50 µg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich GmbH, Schnelldorf, Germany). Upon 70-90% confluence cells were reseeded in a ratio of 1:3. For hMSC-senescent, this procedure was repeated for up to x passages when the hMSC did not become confluent within 3 weeks due to replicative senescence. Control cells were reseeded until passage 1 and 2, respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | At 80-90% confluence human MSC monolayers were lysed. Total RNA was isolated using the NucleoSpin RNA II Purification Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions including DNase digestion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified and labeled according to the GeneChip One-Cycle cDNA Synthesis Kit starting from 2 µg RNA (Affymetrix, High Wycombe, United Kingdom).
| | Sample_hyb_protocol | Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133_Plus_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit.
| | Sample_scan_protocol | Hybridization signals were detected with Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Global scaling was performed by Affymetrix GeneChipOperatingSoftware 1.4 using the MAS5 algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peggy,,Benisch
| | Sample_contact_email | p-benisch.klh@uni-wuerzburg.de
| | Sample_contact_laboratory | Osteology
| | Sample_contact_institute | Orthopedic Center for Musculoskeletal Research
| | Sample_contact_address | Brettreichstr.11
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM878nnn/GSM878113/suppl/GSM878113_hMSC-senescent_donor5.CEL.gz
| | Sample_series_id | GSE35957
| | Sample_series_id | GSE35959
| | Sample_data_row_count | 54675
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