Search results for the GEO ID: GSE36149 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM881823 | GPL570 |
|
RS4:11 Vehicle rep 1
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour vehicle exposure
|
cell line: Acute Lymphoblastic Leukemia cell line RS4:11
agent: vehicle
dose: control
|
|
Sample_geo_accession | GSM881823
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881823/suppl/GSM881823_LLS_FELIX1_01.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881824 | GPL570 |
|
RS4:11 obatoclax EC50 rep 1
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour obatoclax exposure at 72h EC50
|
cell line: Acute Lymphoblastic Leukemia cell line RS4:11
agent: obatoclax
dose: 72h EC50
|
|
Sample_geo_accession | GSM881824
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881824/suppl/GSM881824_LLS_FELIX1_02.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881825 | GPL570 |
|
RS4:11 obatoclax 3xEC50 rep 1
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour obatoclax exposure at 3x 72h EC50
|
cell line: Acute Lymphoblastic Leukemia cell line RS4:11
agent: obatoclax
dose: 3x 72h EC50
|
|
Sample_geo_accession | GSM881825
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881825/suppl/GSM881825_LLS_FELIX1_03.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881826 | GPL570 |
|
SEM-K2 Vehcle rep 1
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour vehicle exposure
|
cell line: Acute Lymphoblastic Leukemia cell line SEM-K2
agent: vehicle
dose: control
|
|
Sample_geo_accession | GSM881826
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881826/suppl/GSM881826_LLS_FELIX1_04.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881827 | GPL570 |
|
SEM-K2 obatoclax EC50 rep 1
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour obatoclax exposure at 72h EC50
|
cell line: Acute Lymphoblastic Leukemia cell line SEM-K2
agent: obatoclax
dose: 72h EC50
|
|
Sample_geo_accession | GSM881827
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881827/suppl/GSM881827_LLS_FELIX1_05.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881828 | GPL570 |
|
SEM-K2 obatoclax 3xEC50 rep 1
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour obatoclax exposure at 3x 72h EC50
|
cell line: Acute Lymphoblastic Leukemia cell line SEM-K2
agent: obatoclax
dose: 3x 72h EC50
|
|
Sample_geo_accession | GSM881828
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881828/suppl/GSM881828_LLS_FELIX1_06.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881829 | GPL570 |
|
RS4:11 Vehicle rep 2
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour vehicle exposure
|
cell line: Acute Lymphoblastic Leukemia cell line RS4:11
agent: vehicle
dose: control
|
|
Sample_geo_accession | GSM881829
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881829/suppl/GSM881829_LLS_FELIX1_07.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881830 | GPL570 |
|
RS4:11 obatoclax EC50 rep 2
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour obatoclax exposure at 72h EC50
|
cell line: Acute Lymphoblastic Leukemia cell line RS4:11
agent: obatoclax
dose: 72h EC50
|
|
Sample_geo_accession | GSM881830
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881830/suppl/GSM881830_LLS_FELIX1_08.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881831 | GPL570 |
|
RS4:11 obatoclax 3xEC50 rep 2
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour obatoclax exposure at 3x 72h EC50
|
cell line: Acute Lymphoblastic Leukemia cell line RS4:11
agent: obatoclax
dose: 3x 72h EC50
|
|
Sample_geo_accession | GSM881831
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881831/suppl/GSM881831_LLS_FELIX1_09.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881832 | GPL570 |
|
SEM-K2 Vehcle rep 2
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour vehicle exposure
|
cell line: Acute Lymphoblastic Leukemia cell line SEM-K2
agent: vehicle
dose: control
|
|
Sample_geo_accession | GSM881832
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881832/suppl/GSM881832_LLS_FELIX1_10.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881833 | GPL570 |
|
SEM-K2 obatoclax EC50 rep 2
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour obatoclax exposure at 72h EC50
|
cell line: Acute Lymphoblastic Leukemia cell line SEM-K2
agent: obatoclax
dose: 72h EC50
|
|
Sample_geo_accession | GSM881833
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881833/suppl/GSM881833_LLS_FELIX1_11.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
| |
|
GSM881834 | GPL570 |
|
SEM-K2 obatoclax 3xEC50 rep 2
|
Human MLL-AF4 acute lymphoblastic leukemia cell line, 6 hour obatoclax exposure at 3x 72h EC50
|
cell line: Acute Lymphoblastic Leukemia cell line SEM-K2
agent: obatoclax
dose: 3x 72h EC50
|
|
Sample_geo_accession | GSM881834
| Sample_status | Public on Apr 24 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Apr 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 24 hours after plating, 10x106 cells were treated with vehicle or obatoclax at the 72 hour EC50 or 3xEC50 for 6 hours (2 replicates per condition).
| Sample_growth_protocol_ch1 | SEM-K2 and RS4:11 cells were plated at 106cell/ml
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed with TRIzol. Total RNA was extracted with chloroform and precipitated with isopropanol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled cRNA was prepared according to Affimetryx protocol (GeneChips Expression Analysis Technical Manual) from 2-2.5 ug of total RNA
| Sample_hyb_protocol | 15 ug of cRNA was hybridized to Affymetrix U133 plus 2.0 Arrays for 16 hours at 45 degrees Celcius
| Sample_scan_protocol | Microarray chips were scanned using a Affymetrix GeneChip Scanner
| Sample_data_processing | The data were analyzed using the R/Bioconductor package GC-RMA with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Li-San,,Wang
| Sample_contact_email | lswang@mail.med.upenn.edu
| Sample_contact_department | Pathology and Laboratory Medicine
| Sample_contact_institute | University of Pennsylvania
| Sample_contact_address | 1424 Blockley Hall, 423 Guardian Drive
| Sample_contact_city | Philadelphia
| Sample_contact_state | PA
| Sample_contact_zip/postal_code | 19104
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM881nnn/GSM881834/suppl/GSM881834_LLS_FELIX1_12.CEL.gz
| Sample_series_id | GSE36149
| Sample_data_row_count | 54675
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