Search results for the GEO ID: GSE36174 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM882304 | GPL85 |
|
Rat Lung at Day 0, biological rep1
|
Rat 8-10 wks exposed to LPS intranasally at Day 0
|
age: 8-10 wks age
Sex: Male
strain: F344/ NCrR
genotype: wild type
|
Gene expression data from lung epithelial cells at Day 0 post LPS exposure
|
Sample_geo_accession | GSM882304
| Sample_status | Public on Mar 01 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Mar 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | For intratracheal instillations, rats were briefly anesthetized with 5% isoflurane in oxygen and instilled intranasally with 1000 μg of LPS (Pseudomonas aeruginosa serotype 10, lot 31K4122, 3,000,000 LPS units (EU)/mg, Sigma, St. Louis, MO) in 0.5 ml of 0.9% pyrogen-free saline. Control rats were not instilled.
| Sample_growth_protocol_ch1 | Specific pathogen-free F344/NCrR male rats of 6–8 wk of age were obtained from NCI (Frederick, MD) and were housed until 8–10 weeks of age. The rats were housed in pairs and were provided food and water ad libitum, a 12-h light/dark cycle at 22.2°C, and 30-40% humidity. Rats were weighed and randomly assigned to each experimental group.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The right lungs of F344/NCrR male rats at 0 and 2 d post LPS instillation were snap-frozen, and stored at -80 C after inflating with diluted (1:4 in PBS) Tissue-Tek O.C.T. (EMS Biosciences, Hatfeild, PA). Frozen tissue sections (8 μm thick) were fixed, dehydrated, air-dried, and epithelial cells from five large airways of each rat were captured using the laser onto Arcturus® CapSure® HS LCM Caps (Applied Biosystems, Foster City, CA). Total RNA was extracted from the cellular lysate in the cap using the PicoPure® RNA Isolation Kit (Applied Biosystems).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | mRNA were amplified using the RiboAmp RNA amplification kit (Applied Biosystems) as per manufacturer’s instructions. Epithelia microdissected from eight tissue sections resulted in an average 1000 ng of amplified RNA. A second round of amplification was carried out and the biotinylated cRNA probes were then prepared.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA (in 200 ul) were hybridized for 16 hr at 45 C on rat microarray chip RG-U34A (Affymetrix Inc., Sunnyvale, CA). Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400. Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
| Sample_scan_protocol | Microarray Chips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 4.0 (MAS 4.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| Sample_platform_id | GPL85
| Sample_contact_name | Hitendra,,Chand
| Sample_contact_email | hchand@lrri.org
| Sample_contact_phone | 505-348-8774
| Sample_contact_institute | Lovelace Respiraotry Research Institute
| Sample_contact_address | 2425 Ridgecrest Dr SE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882304/suppl/GSM882304_Control_1_Metrics.TXT.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882304/suppl/GSM882304_FG_RatAsthma_Control_1_RGU34A.CEL.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882304/suppl/GSM882304_FG_RatAsthma_Control_1_RGU34A.CHP.gz
| Sample_series_id | GSE36174
| Sample_data_row_count | 8799
| |
|
GSM882306 | GPL85 |
|
Rat Lung at Day 0, biological rep2
|
Rat 8-10 wks exposed to LPS intranasally at Day 0
|
age: 8-10 wks age
Sex: Male
strain: F344/ NCrR
genotype: wild type
|
Gene expression data from lung epithelial cells at Day 0 post LPS exposure
|
Sample_geo_accession | GSM882306
| Sample_status | Public on Mar 01 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Mar 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | For intratracheal instillations, rats were briefly anesthetized with 5% isoflurane in oxygen and instilled intranasally with 1000 μg of LPS (Pseudomonas aeruginosa serotype 10, lot 31K4122, 3,000,000 LPS units (EU)/mg, Sigma, St. Louis, MO) in 0.5 ml of 0.9% pyrogen-free saline. Control rats were not instilled.
| Sample_growth_protocol_ch1 | Specific pathogen-free F344/NCrR male rats of 6–8 wk of age were obtained from NCI (Frederick, MD) and were housed until 8–10 weeks of age. The rats were housed in pairs and were provided food and water ad libitum, a 12-h light/dark cycle at 22.2°C, and 30-40% humidity. Rats were weighed and randomly assigned to each experimental group.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The right lungs of F344/NCrR male rats at 0 and 2 d post LPS instillation were snap-frozen, and stored at -80 C after inflating with diluted (1:4 in PBS) Tissue-Tek O.C.T. (EMS Biosciences, Hatfeild, PA). Frozen tissue sections (8 μm thick) were fixed, dehydrated, air-dried, and epithelial cells from five large airways of each rat were captured using the laser onto Arcturus® CapSure® HS LCM Caps (Applied Biosystems, Foster City, CA). Total RNA was extracted from the cellular lysate in the cap using the PicoPure® RNA Isolation Kit (Applied Biosystems).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | mRNA were amplified using the RiboAmp RNA amplification kit (Applied Biosystems) as per manufacturer’s instructions. Epithelia microdissected from eight tissue sections resulted in an average 1000 ng of amplified RNA. A second round of amplification was carried out and the biotinylated cRNA probes were then prepared.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA (in 200 ul) were hybridized for 16 hr at 45 C on rat microarray chip RG-U34A (Affymetrix Inc., Sunnyvale, CA). Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400. Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
| Sample_scan_protocol | Microarray Chips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 4.0 (MAS 4.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| Sample_platform_id | GPL85
| Sample_contact_name | Hitendra,,Chand
| Sample_contact_email | hchand@lrri.org
| Sample_contact_phone | 505-348-8774
| Sample_contact_institute | Lovelace Respiraotry Research Institute
| Sample_contact_address | 2425 Ridgecrest Dr SE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882306/suppl/GSM882306_Control_2_Metrics.TXT.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882306/suppl/GSM882306_FG_RatAsthma_Control_2_RGU34A.CEL.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882306/suppl/GSM882306_FG_RatAsthma_Control_2_RGU34A.CHP.gz
| Sample_series_id | GSE36174
| Sample_data_row_count | 8799
| |
|
GSM882307 | GPL85 |
|
Rat Lung at Day 0, biological rep3
|
Rat 8-10 wks exposed to LPS intranasally at Day 0
|
age: 8-10 wks age
Sex: Male
strain: F344/ NCrR
genotype: wild type
|
Gene expression data from lung epithelial cells at Day 0 post LPS exposure
|
Sample_geo_accession | GSM882307
| Sample_status | Public on Mar 01 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Mar 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | For intratracheal instillations, rats were briefly anesthetized with 5% isoflurane in oxygen and instilled intranasally with 1000 μg of LPS (Pseudomonas aeruginosa serotype 10, lot 31K4122, 3,000,000 LPS units (EU)/mg, Sigma, St. Louis, MO) in 0.5 ml of 0.9% pyrogen-free saline. Control rats were not instilled.
| Sample_growth_protocol_ch1 | Specific pathogen-free F344/NCrR male rats of 6–8 wk of age were obtained from NCI (Frederick, MD) and were housed until 8–10 weeks of age. The rats were housed in pairs and were provided food and water ad libitum, a 12-h light/dark cycle at 22.2°C, and 30-40% humidity. Rats were weighed and randomly assigned to each experimental group.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The right lungs of F344/NCrR male rats at 0 and 2 d post LPS instillation were snap-frozen, and stored at -80 C after inflating with diluted (1:4 in PBS) Tissue-Tek O.C.T. (EMS Biosciences, Hatfeild, PA). Frozen tissue sections (8 μm thick) were fixed, dehydrated, air-dried, and epithelial cells from five large airways of each rat were captured using the laser onto Arcturus® CapSure® HS LCM Caps (Applied Biosystems, Foster City, CA). Total RNA was extracted from the cellular lysate in the cap using the PicoPure® RNA Isolation Kit (Applied Biosystems).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | mRNA were amplified using the RiboAmp RNA amplification kit (Applied Biosystems) as per manufacturer’s instructions. Epithelia microdissected from eight tissue sections resulted in an average 1000 ng of amplified RNA. A second round of amplification was carried out and the biotinylated cRNA probes were then prepared.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA (in 200 ul) were hybridized for 16 hr at 45 C on rat microarray chip RG-U34A (Affymetrix Inc., Sunnyvale, CA). Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400. Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
| Sample_scan_protocol | Microarray Chips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 4.0 (MAS 4.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| Sample_platform_id | GPL85
| Sample_contact_name | Hitendra,,Chand
| Sample_contact_email | hchand@lrri.org
| Sample_contact_phone | 505-348-8774
| Sample_contact_institute | Lovelace Respiraotry Research Institute
| Sample_contact_address | 2425 Ridgecrest Dr SE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882307/suppl/GSM882307_Control_3_Metrics.TXT.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882307/suppl/GSM882307_FG_RatAsthma_Control_3_RGU34A.CEL.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882307/suppl/GSM882307_FG_RatAsthma_Control_3_RGU34A.CHP.gz
| Sample_series_id | GSE36174
| Sample_data_row_count | 8799
| |
|
GSM882309 | GPL85 |
|
Rat Lung at Day 2, biological rep1
|
Rat 8-10 wks exposed to LPS intranasally at Day 2
|
age: 8-10 wks age
Sex: Male
strain: F344/ NCrR
genotype: wild type
|
Gene expression data from lung epithelial cells at Day 2 post LPS exposure
|
Sample_geo_accession | GSM882309
| Sample_status | Public on Mar 01 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Mar 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | For intratracheal instillations, rats were briefly anesthetized with 5% isoflurane in oxygen and instilled intranasally with 1000 μg of LPS (Pseudomonas aeruginosa serotype 10, lot 31K4122, 3,000,000 LPS units (EU)/mg, Sigma, St. Louis, MO) in 0.5 ml of 0.9% pyrogen-free saline. Control rats were not instilled.
| Sample_growth_protocol_ch1 | Specific pathogen-free F344/NCrR male rats of 6–8 wk of age were obtained from NCI (Frederick, MD) and were housed until 8–10 weeks of age. The rats were housed in pairs and were provided food and water ad libitum, a 12-h light/dark cycle at 22.2°C, and 30-40% humidity. Rats were weighed and randomly assigned to each experimental group.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The right lungs of F344/NCrR male rats at 0 and 2 d post LPS instillation were snap-frozen, and stored at -80 C after inflating with diluted (1:4 in PBS) Tissue-Tek O.C.T. (EMS Biosciences, Hatfeild, PA). Frozen tissue sections (8 μm thick) were fixed, dehydrated, air-dried, and epithelial cells from five large airways of each rat were captured using the laser onto Arcturus® CapSure® HS LCM Caps (Applied Biosystems, Foster City, CA). Total RNA was extracted from the cellular lysate in the cap using the PicoPure® RNA Isolation Kit (Applied Biosystems).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | mRNA were amplified using the RiboAmp RNA amplification kit (Applied Biosystems) as per manufacturer’s instructions. Epithelia microdissected from eight tissue sections resulted in an average 1000 ng of amplified RNA. A second round of amplification was carried out and the biotinylated cRNA probes were then prepared.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA (in 200 ul) were hybridized for 16 hr at 45 C on rat microarray chip RG-U34A (Affymetrix Inc., Sunnyvale, CA). Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400. Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
| Sample_scan_protocol | Microarray Chips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 4.0 (MAS 4.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| Sample_platform_id | GPL85
| Sample_contact_name | Hitendra,,Chand
| Sample_contact_email | hchand@lrri.org
| Sample_contact_phone | 505-348-8774
| Sample_contact_institute | Lovelace Respiraotry Research Institute
| Sample_contact_address | 2425 Ridgecrest Dr SE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882309/suppl/GSM882309_2DayAfterLPS_1_metrics.TXT.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882309/suppl/GSM882309_FG_RatAsthma_2DayAfterLPS_1_RGU34A.CEL.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882309/suppl/GSM882309_FG_RatAsthma_2DayAfterLPS_1_RGU34A.CHP.gz
| Sample_series_id | GSE36174
| Sample_data_row_count | 8799
| |
|
GSM882310 | GPL85 |
|
Rat Lung at Day 2, biological rep2
|
Rat 8-10 wks exposed to LPS intranasally at Day 2
|
age: 8-10 wks age
Sex: Male
strain: F344/ NCrR
genotype: wild type
|
Gene expression data from lung epithelial cells at Day 2 post LPS exposure
|
Sample_geo_accession | GSM882310
| Sample_status | Public on Mar 01 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Mar 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | For intratracheal instillations, rats were briefly anesthetized with 5% isoflurane in oxygen and instilled intranasally with 1000 μg of LPS (Pseudomonas aeruginosa serotype 10, lot 31K4122, 3,000,000 LPS units (EU)/mg, Sigma, St. Louis, MO) in 0.5 ml of 0.9% pyrogen-free saline. Control rats were not instilled.
| Sample_growth_protocol_ch1 | Specific pathogen-free F344/NCrR male rats of 6–8 wk of age were obtained from NCI (Frederick, MD) and were housed until 8–10 weeks of age. The rats were housed in pairs and were provided food and water ad libitum, a 12-h light/dark cycle at 22.2°C, and 30-40% humidity. Rats were weighed and randomly assigned to each experimental group.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The right lungs of F344/NCrR male rats at 0 and 2 d post LPS instillation were snap-frozen, and stored at -80 C after inflating with diluted (1:4 in PBS) Tissue-Tek O.C.T. (EMS Biosciences, Hatfeild, PA). Frozen tissue sections (8 μm thick) were fixed, dehydrated, air-dried, and epithelial cells from five large airways of each rat were captured using the laser onto Arcturus® CapSure® HS LCM Caps (Applied Biosystems, Foster City, CA). Total RNA was extracted from the cellular lysate in the cap using the PicoPure® RNA Isolation Kit (Applied Biosystems).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | mRNA were amplified using the RiboAmp RNA amplification kit (Applied Biosystems) as per manufacturer’s instructions. Epithelia microdissected from eight tissue sections resulted in an average 1000 ng of amplified RNA. A second round of amplification was carried out and the biotinylated cRNA probes were then prepared.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA (in 200 ul) were hybridized for 16 hr at 45 C on rat microarray chip RG-U34A (Affymetrix Inc., Sunnyvale, CA). Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400. Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
| Sample_scan_protocol | Microarray Chips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 4.0 (MAS 4.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| Sample_platform_id | GPL85
| Sample_contact_name | Hitendra,,Chand
| Sample_contact_email | hchand@lrri.org
| Sample_contact_phone | 505-348-8774
| Sample_contact_institute | Lovelace Respiraotry Research Institute
| Sample_contact_address | 2425 Ridgecrest Dr SE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882310/suppl/GSM882310_2DayAfterLPS_2_metrics.TXT.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882310/suppl/GSM882310_FG_RatAsthma_2DayAfterLPS_2_RGU34A.CEL.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882310/suppl/GSM882310_FG_RatAsthma_2DayAfterLPS_2_RGU34A.CHP.gz
| Sample_series_id | GSE36174
| Sample_data_row_count | 8799
| |
|
GSM882312 | GPL85 |
|
Rat Lung at Day 2, biological rep3
|
Rat 8-10 wks exposed to LPS intranasally at Day 2
|
age: 8-10 wks age
Sex: Male
strain: F344/ NCrR
genotype: wild type
|
Gene expression data from lung epithelial cells at Day 2 post LPS exposure
|
Sample_geo_accession | GSM882312
| Sample_status | Public on Mar 01 2012
| Sample_submission_date | Feb 29 2012
| Sample_last_update_date | Mar 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | For intratracheal instillations, rats were briefly anesthetized with 5% isoflurane in oxygen and instilled intranasally with 1000 μg of LPS (Pseudomonas aeruginosa serotype 10, lot 31K4122, 3,000,000 LPS units (EU)/mg, Sigma, St. Louis, MO) in 0.5 ml of 0.9% pyrogen-free saline. Control rats were not instilled.
| Sample_growth_protocol_ch1 | Specific pathogen-free F344/NCrR male rats of 6–8 wk of age were obtained from NCI (Frederick, MD) and were housed until 8–10 weeks of age. The rats were housed in pairs and were provided food and water ad libitum, a 12-h light/dark cycle at 22.2°C, and 30-40% humidity. Rats were weighed and randomly assigned to each experimental group.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The right lungs of F344/NCrR male rats at 0 and 2 d post LPS instillation were snap-frozen, and stored at -80 C after inflating with diluted (1:4 in PBS) Tissue-Tek O.C.T. (EMS Biosciences, Hatfeild, PA). Frozen tissue sections (8 μm thick) were fixed, dehydrated, air-dried, and epithelial cells from five large airways of each rat were captured using the laser onto Arcturus® CapSure® HS LCM Caps (Applied Biosystems, Foster City, CA). Total RNA was extracted from the cellular lysate in the cap using the PicoPure® RNA Isolation Kit (Applied Biosystems).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | mRNA were amplified using the RiboAmp RNA amplification kit (Applied Biosystems) as per manufacturer’s instructions. Epithelia microdissected from eight tissue sections resulted in an average 1000 ng of amplified RNA. A second round of amplification was carried out and the biotinylated cRNA probes were then prepared.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA (in 200 ul) were hybridized for 16 hr at 45 C on rat microarray chip RG-U34A (Affymetrix Inc., Sunnyvale, CA). Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400. Microarray Chips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
| Sample_scan_protocol | Microarray Chips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 4.0 (MAS 4.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
| Sample_platform_id | GPL85
| Sample_contact_name | Hitendra,,Chand
| Sample_contact_email | hchand@lrri.org
| Sample_contact_phone | 505-348-8774
| Sample_contact_institute | Lovelace Respiraotry Research Institute
| Sample_contact_address | 2425 Ridgecrest Dr SE
| Sample_contact_city | Albuquerque
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882312/suppl/GSM882312_2DayAfterLPS_3_metrics.TXT.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882312/suppl/GSM882312_FG_RatAsthma_2DayAfterLPS_3_RGU34A.CEL.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM882nnn/GSM882312/suppl/GSM882312_FG_RatAsthma_2DayAfterLPS_3_RGU34A.CHP.gz
| Sample_series_id | GSE36174
| Sample_data_row_count | 8799
| |
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