Search results for the GEO ID: GSE36209 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM883715 | GPL1261 |
|
podocyte from OVE26 diabetic mouse 1
|
podocyte from mouse kidney
|
genotype/variation: OVE-GFP
background strain: FVB
age: 3-4 months
disease state: diabetic
|
gene expression data for OVE26 podocyte
|
Sample_geo_accession | GSM883715
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | Mar 01 2012
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Glomeruli were digested by 0.25% trypsin solution in 37oC, 5%CO2 incubator. Dispersed cell cluster by 1ml pipette until all fluorescence podocyte into singal cell. Washed cell and filtered by 35um cell strain then applied to FACS cell sorting machine.
| Sample_growth_protocol_ch1 | Mouse kidney was perfused by magnetic bead and kidney was digested by 1mg/ml collagenase solution. Glomeruli were isolated and purified by various magnetic platforms.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was extracted by PicoPure RNA isolation kti from Arcturus according to manufacture's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | NuGen FL-Ovation and Biotin Labeling Kit (Nugen Inc., San Carlos, CA)
| Sample_hyb_protocol | Hybridization: 45 °C for 18 hours, Wash Protocol FS450_0004 on FS450.
| Sample_scan_protocol | Affymetrix, GeneChip Scanner 30007G, GCOS 1.4
| Sample_data_processing | Partek Genomics Suite, RMA Summarization and Normalization, 1-Way ANOVA: Diabetic vs Normal
| Sample_platform_id | GPL1261
| Sample_contact_name | Jianxiang ,,Xu
| Sample_contact_email | j0xu0006@louisville.edu
| Sample_contact_phone | 502 852 2650
| Sample_contact_fax | 502 852 5634
| Sample_contact_laboratory | KCHRI diabetes
| Sample_contact_department | Pediatrics
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston ST Suite 304
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM883nnn/GSM883715/suppl/GSM883715.CEL.gz
| Sample_series_id | GSE36209
| Sample_data_row_count | 45101
| |
|
GSM883716 | GPL1261 |
|
podocyte from OVE26 diabetic mouse 2
|
podocyte from mouse kidney
|
genotype/variation: OVE-GFP
background strain: FVB
age: 3-4 months
disease state: diabetic
|
gene expression data for OVE26 podocyte
|
Sample_geo_accession | GSM883716
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | Mar 01 2012
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Glomeruli were digested by 0.25% trypsin solution in 37oC, 5%CO2 incubator. Dispersed cell cluster by 1ml pipette until all fluorescence podocyte into singal cell. Washed cell and filtered by 35um cell strain then applied to FACS cell sorting machine.
| Sample_growth_protocol_ch1 | Mouse kidney was perfused by magnetic bead and kidney was digested by 1mg/ml collagenase solution. Glomeruli were isolated and purified by various magnetic platforms.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was extracted by PicoPure RNA isolation kti from Arcturus according to manufacture's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | NuGen FL-Ovation and Biotin Labeling Kit (Nugen Inc., San Carlos, CA)
| Sample_hyb_protocol | Hybridization: 45 °C for 18 hours, Wash Protocol FS450_0004 on FS450.
| Sample_scan_protocol | Affymetrix, GeneChip Scanner 30007G, GCOS 1.4
| Sample_data_processing | Partek Genomics Suite, RMA Summarization and Normalization, 1-Way ANOVA: Diabetic vs Normal
| Sample_platform_id | GPL1261
| Sample_contact_name | Jianxiang ,,Xu
| Sample_contact_email | j0xu0006@louisville.edu
| Sample_contact_phone | 502 852 2650
| Sample_contact_fax | 502 852 5634
| Sample_contact_laboratory | KCHRI diabetes
| Sample_contact_department | Pediatrics
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston ST Suite 304
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM883nnn/GSM883716/suppl/GSM883716.CEL.gz
| Sample_series_id | GSE36209
| Sample_data_row_count | 45101
| |
|
GSM883717 | GPL1261 |
|
podocyte from OVE26 diabetic mouse 3
|
podocyte from mouse kidney
|
genotype/variation: OVE-GFP
background strain: FVB
age: 3-4 months
disease state: diabetic
|
gene expression data for OVE26 podocyte
|
Sample_geo_accession | GSM883717
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | Mar 01 2012
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Glomeruli were digested by 0.25% trypsin solution in 37oC, 5%CO2 incubator. Dispersed cell cluster by 1ml pipette until all fluorescence podocyte into singal cell. Washed cell and filtered by 35um cell strain then applied to FACS cell sorting machine.
| Sample_growth_protocol_ch1 | Mouse kidney was perfused by magnetic bead and kidney was digested by 1mg/ml collagenase solution. Glomeruli were isolated and purified by various magnetic platforms.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was extracted by PicoPure RNA isolation kti from Arcturus according to manufacture's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | NuGen FL-Ovation and Biotin Labeling Kit (Nugen Inc., San Carlos, CA)
| Sample_hyb_protocol | Hybridization: 45 °C for 18 hours, Wash Protocol FS450_0004 on FS450.
| Sample_scan_protocol | Affymetrix, GeneChip Scanner 30007G, GCOS 1.4
| Sample_data_processing | Partek Genomics Suite, RMA Summarization and Normalization, 1-Way ANOVA: Diabetic vs Normal
| Sample_platform_id | GPL1261
| Sample_contact_name | Jianxiang ,,Xu
| Sample_contact_email | j0xu0006@louisville.edu
| Sample_contact_phone | 502 852 2650
| Sample_contact_fax | 502 852 5634
| Sample_contact_laboratory | KCHRI diabetes
| Sample_contact_department | Pediatrics
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston ST Suite 304
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM883nnn/GSM883717/suppl/GSM883717.CEL.gz
| Sample_series_id | GSE36209
| Sample_data_row_count | 45101
| |
|
GSM883718 | GPL1261 |
|
podocyte from OVE26 diabetic mouse 4
|
podocyte from mouse kidney
|
genotype/variation: OVE-GFP
background strain: FVB
age: 3-4 months
disease state: diabetic
|
gene expression data for OVE26 podocyte
|
Sample_geo_accession | GSM883718
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | Mar 01 2012
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Glomeruli were digested by 0.25% trypsin solution in 37oC, 5%CO2 incubator. Dispersed cell cluster by 1ml pipette until all fluorescence podocyte into singal cell. Washed cell and filtered by 35um cell strain then applied to FACS cell sorting machine.
| Sample_growth_protocol_ch1 | Mouse kidney was perfused by magnetic bead and kidney was digested by 1mg/ml collagenase solution. Glomeruli were isolated and purified by various magnetic platforms.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was extracted by PicoPure RNA isolation kti from Arcturus according to manufacture's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | NuGen FL-Ovation and Biotin Labeling Kit (Nugen Inc., San Carlos, CA)
| Sample_hyb_protocol | Hybridization: 45 °C for 18 hours, Wash Protocol FS450_0004 on FS450.
| Sample_scan_protocol | Affymetrix, GeneChip Scanner 30007G, GCOS 1.4
| Sample_data_processing | Partek Genomics Suite, RMA Summarization and Normalization, 1-Way ANOVA: Diabetic vs Normal
| Sample_platform_id | GPL1261
| Sample_contact_name | Jianxiang ,,Xu
| Sample_contact_email | j0xu0006@louisville.edu
| Sample_contact_phone | 502 852 2650
| Sample_contact_fax | 502 852 5634
| Sample_contact_laboratory | KCHRI diabetes
| Sample_contact_department | Pediatrics
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston ST Suite 304
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM883nnn/GSM883718/suppl/GSM883718.CEL.gz
| Sample_series_id | GSE36209
| Sample_data_row_count | 45101
| |
|
GSM883719 | GPL1261 |
|
podocyte from OVE26 diabetic mouse 5
|
podocyte from mouse kidney
|
genotype/variation: OVE-GFP
background strain: FVB
age: 3-4 months
disease state: diabetic
|
gene expression data for OVE26 podocyte
|
Sample_geo_accession | GSM883719
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | Mar 01 2012
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Glomeruli were digested by 0.25% trypsin solution in 37oC, 5%CO2 incubator. Dispersed cell cluster by 1ml pipette until all fluorescence podocyte into singal cell. Washed cell and filtered by 35um cell strain then applied to FACS cell sorting machine.
| Sample_growth_protocol_ch1 | Mouse kidney was perfused by magnetic bead and kidney was digested by 1mg/ml collagenase solution. Glomeruli were isolated and purified by various magnetic platforms.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was extracted by PicoPure RNA isolation kti from Arcturus according to manufacture's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | NuGen FL-Ovation and Biotin Labeling Kit (Nugen Inc., San Carlos, CA)
| Sample_hyb_protocol | Hybridization: 45 °C for 18 hours, Wash Protocol FS450_0004 on FS450.
| Sample_scan_protocol | Affymetrix, GeneChip Scanner 30007G, GCOS 1.4
| Sample_data_processing | Partek Genomics Suite, RMA Summarization and Normalization, 1-Way ANOVA: Diabetic vs Normal
| Sample_platform_id | GPL1261
| Sample_contact_name | Jianxiang ,,Xu
| Sample_contact_email | j0xu0006@louisville.edu
| Sample_contact_phone | 502 852 2650
| Sample_contact_fax | 502 852 5634
| Sample_contact_laboratory | KCHRI diabetes
| Sample_contact_department | Pediatrics
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston ST Suite 304
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM883nnn/GSM883719/suppl/GSM883719.CEL.gz
| Sample_series_id | GSE36209
| Sample_data_row_count | 45101
| |
|
GSM883720 | GPL1261 |
|
podocyte from normal FVB mouse 1
|
podocyte from mouse kidney
|
genotype/variation: wild type
background strain: FVB
age: 3-4 months
disease state: normal
|
gene expression data for FVB podocyte
|
Sample_geo_accession | GSM883720
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | Mar 01 2012
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Glomeruli were digested by 0.25% trypsin solution in 37oC, 5%CO2 incubator. Dispersed cell cluster by 1ml pipette until all fluorescence podocyte into singal cell. Washed cell and filtered by 35um cell strain then applied to FACS cell sorting machine.
| Sample_growth_protocol_ch1 | Mouse kidney was perfused by magnetic bead and kidney was digested by 1mg/ml collagenase solution. Glomeruli were isolated and purified by various magnetic platforms.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was extracted by PicoPure RNA isolation kti from Arcturus according to manufacture's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | NuGen FL-Ovation and Biotin Labeling Kit (Nugen Inc., San Carlos, CA)
| Sample_hyb_protocol | Hybridization: 45 °C for 18 hours, Wash Protocol FS450_0004 on FS450.
| Sample_scan_protocol | Affymetrix, GeneChip Scanner 30007G, GCOS 1.4
| Sample_data_processing | Partek Genomics Suite, RMA Summarization and Normalization, 1-Way ANOVA: Diabetic vs Normal
| Sample_platform_id | GPL1261
| Sample_contact_name | Jianxiang ,,Xu
| Sample_contact_email | j0xu0006@louisville.edu
| Sample_contact_phone | 502 852 2650
| Sample_contact_fax | 502 852 5634
| Sample_contact_laboratory | KCHRI diabetes
| Sample_contact_department | Pediatrics
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston ST Suite 304
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM883nnn/GSM883720/suppl/GSM883720.CEL.gz
| Sample_series_id | GSE36209
| Sample_data_row_count | 45101
| |
|
GSM883721 | GPL1261 |
|
podocyte from normal FVB mouse 2
|
podocyte from mouse kidney
|
genotype/variation: wild type
background strain: FVB
age: 3-4 months
disease state: normal
|
gene expression data for FVB podocyte
|
Sample_geo_accession | GSM883721
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | Mar 01 2012
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Glomeruli were digested by 0.25% trypsin solution in 37oC, 5%CO2 incubator. Dispersed cell cluster by 1ml pipette until all fluorescence podocyte into singal cell. Washed cell and filtered by 35um cell strain then applied to FACS cell sorting machine.
| Sample_growth_protocol_ch1 | Mouse kidney was perfused by magnetic bead and kidney was digested by 1mg/ml collagenase solution. Glomeruli were isolated and purified by various magnetic platforms.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was extracted by PicoPure RNA isolation kti from Arcturus according to manufacture's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | NuGen FL-Ovation and Biotin Labeling Kit (Nugen Inc., San Carlos, CA)
| Sample_hyb_protocol | Hybridization: 45 °C for 18 hours, Wash Protocol FS450_0004 on FS450.
| Sample_scan_protocol | Affymetrix, GeneChip Scanner 30007G, GCOS 1.4
| Sample_data_processing | Partek Genomics Suite, RMA Summarization and Normalization, 1-Way ANOVA: Diabetic vs Normal
| Sample_platform_id | GPL1261
| Sample_contact_name | Jianxiang ,,Xu
| Sample_contact_email | j0xu0006@louisville.edu
| Sample_contact_phone | 502 852 2650
| Sample_contact_fax | 502 852 5634
| Sample_contact_laboratory | KCHRI diabetes
| Sample_contact_department | Pediatrics
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston ST Suite 304
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM883nnn/GSM883721/suppl/GSM883721.CEL.gz
| Sample_series_id | GSE36209
| Sample_data_row_count | 45101
| |
|
GSM883722 | GPL1261 |
|
podocyte from normal FVB mouse 3
|
podocyte from mouse kidney
|
genotype/variation: wild type
background strain: FVB
age: 3-4 months
disease state: normal
|
gene expression data for FVB podocyte
|
Sample_geo_accession | GSM883722
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | Mar 01 2012
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Glomeruli were digested by 0.25% trypsin solution in 37oC, 5%CO2 incubator. Dispersed cell cluster by 1ml pipette until all fluorescence podocyte into singal cell. Washed cell and filtered by 35um cell strain then applied to FACS cell sorting machine.
| Sample_growth_protocol_ch1 | Mouse kidney was perfused by magnetic bead and kidney was digested by 1mg/ml collagenase solution. Glomeruli were isolated and purified by various magnetic platforms.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was extracted by PicoPure RNA isolation kti from Arcturus according to manufacture's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | NuGen FL-Ovation and Biotin Labeling Kit (Nugen Inc., San Carlos, CA)
| Sample_hyb_protocol | Hybridization: 45 °C for 18 hours, Wash Protocol FS450_0004 on FS450.
| Sample_scan_protocol | Affymetrix, GeneChip Scanner 30007G, GCOS 1.4
| Sample_data_processing | Partek Genomics Suite, RMA Summarization and Normalization, 1-Way ANOVA: Diabetic vs Normal
| Sample_platform_id | GPL1261
| Sample_contact_name | Jianxiang ,,Xu
| Sample_contact_email | j0xu0006@louisville.edu
| Sample_contact_phone | 502 852 2650
| Sample_contact_fax | 502 852 5634
| Sample_contact_laboratory | KCHRI diabetes
| Sample_contact_department | Pediatrics
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston ST Suite 304
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM883nnn/GSM883722/suppl/GSM883722.CEL.gz
| Sample_series_id | GSE36209
| Sample_data_row_count | 45101
| |
|
GSM883723 | GPL1261 |
|
podocyte from normal FVB mouse 4
|
podocyte from mouse kidney
|
genotype/variation: wild type
background strain: FVB
age: 3-4 months
disease state: normal
|
gene expression data for FVB podocyte
|
Sample_geo_accession | GSM883723
| Sample_status | Public on Mar 02 2012
| Sample_submission_date | Mar 01 2012
| Sample_last_update_date | Mar 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Glomeruli were digested by 0.25% trypsin solution in 37oC, 5%CO2 incubator. Dispersed cell cluster by 1ml pipette until all fluorescence podocyte into singal cell. Washed cell and filtered by 35um cell strain then applied to FACS cell sorting machine.
| Sample_growth_protocol_ch1 | Mouse kidney was perfused by magnetic bead and kidney was digested by 1mg/ml collagenase solution. Glomeruli were isolated and purified by various magnetic platforms.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was extracted by PicoPure RNA isolation kti from Arcturus according to manufacture's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | NuGen FL-Ovation and Biotin Labeling Kit (Nugen Inc., San Carlos, CA)
| Sample_hyb_protocol | Hybridization: 45 °C for 18 hours, Wash Protocol FS450_0004 on FS450.
| Sample_scan_protocol | Affymetrix, GeneChip Scanner 30007G, GCOS 1.4
| Sample_data_processing | Partek Genomics Suite, RMA Summarization and Normalization, 1-Way ANOVA: Diabetic vs Normal
| Sample_platform_id | GPL1261
| Sample_contact_name | Jianxiang ,,Xu
| Sample_contact_email | j0xu0006@louisville.edu
| Sample_contact_phone | 502 852 2650
| Sample_contact_fax | 502 852 5634
| Sample_contact_laboratory | KCHRI diabetes
| Sample_contact_department | Pediatrics
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston ST Suite 304
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM883nnn/GSM883723/suppl/GSM883723.CEL.gz
| Sample_series_id | GSE36209
| Sample_data_row_count | 45101
| |
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