Search results for the GEO ID: GSE36287  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM886421 | GPL570 |
|
Keratinocytes_untreated_subject1
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: untreated control
|
primary human keratinocytes
|
| Sample_geo_accession | GSM886421
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886421/suppl/GSM886421.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886422 | GPL570 |
|
Keratinocytes_untreated_subject2
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: untreated control
|
primary human keratinocytes
|
| Sample_geo_accession | GSM886422
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886422/suppl/GSM886422.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886423 | GPL570 |
|
Keratinocytes_untreated_subject3
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: untreated control
|
primary human keratinocytes
|
| Sample_geo_accession | GSM886423
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886423/suppl/GSM886423.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886424 | GPL570 |
|
Keratinocytes_IFNa_subject1
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IFNa-treated
|
primary human keratinocytes treated with IFNa
|
| Sample_geo_accession | GSM886424
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886424/suppl/GSM886424.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886425 | GPL570 |
|
Keratinocytes_IFNa_subject2
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IFNa-treated
|
primary human keratinocytes treated with IFNa
|
| Sample_geo_accession | GSM886425
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886425/suppl/GSM886425.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886426 | GPL570 |
|
Keratinocytes_IFNa_subject3
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IFNa-treated
|
primary human keratinocytes treated with IFNa
|
| Sample_geo_accession | GSM886426
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886426/suppl/GSM886426.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886427 | GPL570 |
|
Keratinocytes_IFNg_subject1
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IFNg-treated
|
primary human keratinocytes treated with IFNg
|
| Sample_geo_accession | GSM886427
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886427/suppl/GSM886427.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886428 | GPL570 |
|
Keratinocytes_IFNg_subject2
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IFNg-treated
|
primary human keratinocytes treated with IFNg
|
| Sample_geo_accession | GSM886428
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886428/suppl/GSM886428.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886429 | GPL570 |
|
Keratinocytes_IFNg_subject3
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IFNg-treated
|
primary human keratinocytes treated with IFNg
|
| Sample_geo_accession | GSM886429
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886429/suppl/GSM886429.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886430 | GPL570 |
|
Keratinocytes_IL13_subject1
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IL13-treated
|
primary human keratinocytes treated with IL13
|
| Sample_geo_accession | GSM886430
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886430/suppl/GSM886430.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886431 | GPL570 |
|
Keratinocytes_IL13_subject2
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IL13-treated
|
primary human keratinocytes treated with IL13
|
| Sample_geo_accession | GSM886431
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886431/suppl/GSM886431.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886432 | GPL570 |
|
Keratinocytes_IL13_subject3
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IL13-treated
|
primary human keratinocytes treated with IL13
|
| Sample_geo_accession | GSM886432
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886432/suppl/GSM886432.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886433 | GPL570 |
|
Keratinocytes_untreated_replicate1
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: untreated control
|
primary human keratinocytes
|
| Sample_geo_accession | GSM886433
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886433/suppl/GSM886433.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886434 | GPL570 |
|
Keratinocytes_untreated_replicate2
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: untreated control
|
primary human keratinocytes
|
| Sample_geo_accession | GSM886434
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886434/suppl/GSM886434.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886435 | GPL570 |
|
Keratinocytes_untreated_replicate3
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: untreated control
|
primary human keratinocytes
|
| Sample_geo_accession | GSM886435
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886435/suppl/GSM886435.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886436 | GPL570 |
|
Keratinocytes_IL17A_replicate1
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IL17A-treated
|
primary human keratinocytes treated with IL17A
|
| Sample_geo_accession | GSM886436
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886436/suppl/GSM886436.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886437 | GPL570 |
|
Keratinocytes_IL17A_replicate2
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IL17A-treated
|
primary human keratinocytes treated with IL17A
|
| Sample_geo_accession | GSM886437
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886437/suppl/GSM886437.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886438 | GPL570 |
|
Keratinocytes_IL17A_replicate3
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IL17A-treated
|
primary human keratinocytes treated with IL17A
|
| Sample_geo_accession | GSM886438
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886438/suppl/GSM886438.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886439 | GPL570 |
|
Keratinocytes_IL4_subject1
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IL4-treated
|
primary human keratinocytes treated with IL4
|
| Sample_geo_accession | GSM886439
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886439/suppl/GSM886439.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886440 | GPL570 |
|
Keratinocytes_IL4_subject2
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IL4-treated
|
primary human keratinocytes treated with IL4
|
| Sample_geo_accession | GSM886440
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886440/suppl/GSM886440.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886441 | GPL570 |
|
Keratinocytes_IL4_subject3
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: IL4-treated
|
primary human keratinocytes treated with IL4
|
| Sample_geo_accession | GSM886441
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886441/suppl/GSM886441.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886442 | GPL570 |
|
Keratinocytes_TNF_subject1
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: TNF-treated
|
primary human keratinocytes treated with TNF
|
| Sample_geo_accession | GSM886442
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886442/suppl/GSM886442.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886443 | GPL570 |
|
Keratinocytes_TNF_subject2
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: TNF-treated
|
primary human keratinocytes treated with TNF
|
| Sample_geo_accession | GSM886443
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886443/suppl/GSM886443.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
GSM886444 | GPL570 |
|
Keratinocytes_TNF_subject3
|
primary keratinocytes
|
tissue: primary keratinocytes
treatment: TNF-treated
|
primary human keratinocytes treated with TNF
|
| Sample_geo_accession | GSM886444
| | Sample_status | Public on Mar 07 2012
| | Sample_submission_date | Mar 05 2012
| | Sample_last_update_date | Mar 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
| | Sample_growth_protocol_ch1 | Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNEasy mini kits
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
| | Sample_data_processing | Robust Multichip Average (RMA)
| | Sample_platform_id | GPL570
| | Sample_contact_name | William,R,Swindell
| | Sample_contact_email | wswindell@genetics.med.harvard.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Harvard University
| | Sample_contact_address | 77 Avenue Louis Pasteur
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM886nnn/GSM886444/suppl/GSM886444.CEL.gz
| | Sample_series_id | GSE36287
| | Sample_data_row_count | 54675
| | |
|
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